公开(公告)号：EP4306532A3

公开(公告)日：2024-04-10

申请号：EP23203880.2

申请日：2019-05-14

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： WU, Xiaolin ,  SMITH, Randall ,  SHIEH, Peyton ,  BEIERLE, John M. ,  GEORGE, Wayne N. ,  LAWRENCE, Elliot John ,  MAO, Jie ,  LIU, Xiaohai

IPC分类号： C12Q1/6806 ,  C12Q1/6874 ,  C07H1/00 ,  C07H19/10 ,  C07H19/20 ,  C07H21/04

摘要： Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na 2 S 2 O 4 . Nucleotides and oligonucleotides comprising a 3' phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：EP3794012A1

公开(公告)日：2021-03-24

申请号：EP19727252.9

申请日：2019-05-14

申请人： Illumina Inc. ,  Illumina Cambridge Limited

发明人： WU, Xiaolin ,  SMITH, Randall ,  SHIEH, Peyton ,  BEIERLE, John M. ,  GEORGE, Wayne N. ,  LAWRENCE, Elliot John ,  MAO, Jie ,  LIU, Xiaohai

IPC分类号： C07H21/04 ,  C12Q1/6806 ,  C12Q1/6874

公开(公告)号：EP4306532A2

公开(公告)日：2024-01-17

申请号：EP23203880.2

申请日：2019-05-14

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： WU, Xiaolin ,  SMITH, Randall ,  SHIEH, Peyton ,  BEIERLE, John M. ,  GEORGE, Wayne N. ,  LAWRENCE, Elliot John ,  MAO, Jie ,  LIU, Xiaohai

IPC分类号： C07H19/20

摘要： Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na 2 S 2 O 4 . Nucleotides and oligonucleotides comprising a 3' phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：EP4274857A1

公开(公告)日：2023-11-15

申请号：EP21852112.8

申请日：2021-12-30

申请人： Illumina Inc ,  Illumina Cambridge Limited

发明人： SMITH, Randall ,  GEORGE, Wayne ,  BROWN, Andrew

IPC分类号： C08F8/00 ,  C08F8/30

公开(公告)号：EP3981886A1

公开(公告)日：2022-04-13

申请号：EP21189843.2

申请日：2012-09-21

申请人： Illumina, Inc.

发明人： BOUTELL, Jonathan Mark ,  ELTOUKHY, Helmy A. ,  FENG, Wenyi ,  HIRSCHBEIN, Bernard ,  JOSEPH, Thomas ,  KAIN, Robert C. ,  KLAUSING, Kay ,  LIU, Xiaohai ,  SHEN, Min-Jui Richard ,  SMITH, Geoffrey Paul ,  TREGIDGO, Carolyn ,  WU, Xiaolin ,  SMITH, Randall

IPC分类号： C12Q1/6869 ,  C12Q1/6874

摘要： The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected. Disclosed is also a reversibly blocked nucleotide conjugate, which is indirectly labelled with a hapten attached via a linker to the nucleobase, wherein the hapten is detectable by a binding partner-fluorescent moiety conjugate.

公开(公告)号：EP3978908A1

公开(公告)日：2022-04-06

申请号：EP21208877.7

申请日：2018-11-30

申请人： Illumina, Inc.

发明人： FUNG, Tracy, H ,  SABOUNCHI, Poorya ,  HIRSCHBEIN, Bernard ,  PINTO, Joseph ,  KHURANA, Tarun ,  SMITH, Randall ,  FENG, Wenyi

IPC分类号： G01N21/64 ,  G02B5/22 ,  H01L27/146 ,  G02B19/00

摘要： A device comprising: a structure (260) defining a detector surface (206) for supporting biological or chemical samples; a sensor array (201) comprising light sensors (202), and circuitry to transmit data signals based on photons detected by the light sensors (202); and a guide array comprising light guides (214); wherein light guides (214) of the guide array receive excitation light (101) and
emissions signal light (501) from the detector surface (206), wherein the light guides (214) extend toward respective light sensors (202) of the sensor array (201) and comprise filter material that blocks the excitation light and permits the emissions signal light to propagate toward the respective light sensors (202), wherein the detector surface (206) includes a reaction recess (210), the reaction recess comprising an index of refraction and a dimension sufficient to cancel background light energy incident on the detector surface in a detection band of the sensor array. A method comprising steps for fabricating the device.

公开(公告)号：EP3290528A1

公开(公告)日：2018-03-07

申请号：EP17180583.1

申请日：2012-09-21

申请人： Illumina, Inc.

发明人： BOUTELL, Jonathan Mark ,  ELTOUKHY, Helmy A. ,  FENG, Wenyl ,  HIRSCHBEIN, Bernard ,  JOSEPH, Thomas ,  KAIN, Robert C. ,  KLAUSING, Kay ,  LIU, Xiaohai ,  SHEN, Min-Jui Richard ,  SMITH, Geoffrey Paul ,  SMITH, Randall ,  TREGIDGO, Carolyn ,  WU, Xiaolin

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  C12Q2525/113 ,  C12Q2535/122 ,  C12Q2563/107

摘要： The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

公开(公告)号：EP3732468A1

公开(公告)日：2020-11-04

申请号：EP18896064.5

申请日：2018-11-30

申请人： Illumina Inc.

发明人： FUNG, Tracy H. ,  SABOUNCHI, Poorya ,  HIRSCHBEIN, Bernard ,  PINTO, Joseph ,  KHURANA, Tarun ,  SMITH, Randall ,  FENG, Wenyi

IPC分类号： G01N21/64

公开(公告)号：EP3559262A1

公开(公告)日：2019-10-30

申请号：EP17883315.8

申请日：2017-12-20

申请人： Illumina, Inc.

发明人： SHIEH, Peyton ,  BEIERLE, John M. ,  GRAIGE, Michael S. ,  FUHRMANN, Alexander ,  SMITH, Randall ,  WEI, Wei ,  ZHAN, Naiqian

IPC分类号： C12Q1/68 ,  B01J19/00

公开(公告)号：EP2768972B1

公开(公告)日：2017-07-12

申请号：EP12766838.2

申请日：2012-09-21

申请人： Illumina, Inc.

发明人： KAIN, Robert C. ,  LIU, Xiaohai ,  FENG, Wenyi ,  HIRSCHBEIN, Bernard ,  ELTOUKHY, Helmy A. ,  WU, Xiaolin ,  SMITH, Geoffrey Paul ,  BOUTELL, Jonathan Mark ,  JOSEPH, Thomas ,  SMITH, Randall ,  SHEN, Min-Jui Richard ,  TREGIDGO, Carolyn ,  KLAUSING, Kay

IPC分类号： C12Q1/68

摘要： The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

摘要翻译： 本公开提供了用于检测样品中的多个不同核苷酸的方法和系统。 特别地，本公开内容提供了利用比被检测的核苷酸的数量少的检测部分和/或比被检测的核苷酸的数量少的成像事件来检测样品中的多个不同核苷酸。