公开(公告)号：EP1905827A1

公开(公告)日：2008-04-02

申请号：EP06019705.0

申请日：2006-09-20

申请人： Institut Pasteur Korea

发明人： Emans, Neil ,  Nehrbass, Ulf

IPC分类号： C12N15/10

CPC分类号： C12N15/1086 ,  C12Q1/6897

摘要： The present invention relates to a method of detecting and/or quantifying expression of a target protein candidate in a cell, and to a method of identifying a target protein of a small molecule modulator.

公开(公告)号：EP1865320A3

公开(公告)日：2008-02-20

申请号：EP07252322.8

申请日：2007-06-08

申请人： Institut Pasteur Korea

发明人： Emans, Neil ,  Altschuler, Yoram ,  Kwon, Yongjun

IPC分类号： G01N33/50 ,  G01N33/74 ,  C07K14/705

CPC分类号： G01N33/5035 ,  G01N33/5008 ,  G01N33/566 ,  G01N2333/5754 ,  G01N2333/70571 ,  G01N2333/726 ,  G01N2800/12 ,  G01N2800/32

摘要： A method for identifying a compound that modifies the cellular distribution of a GPCR in a population of cells comprising:
a) taking a population of cells expressing a GPCR
b) incubating said cells with a candidate compound and with a ligand of the GPCR
c) determining distribution of said GPCR in cells treated according to step b) and comparing with distribution of GPCR in cells incubated with a ligand of the GPCR in the absence of the candidate compound;

wherein altered distribution in cells incubated with the candidate compound is indicative that the candidate compound modifies GPCR cellular distribution.

公开(公告)号：EP1865320A2

公开(公告)日：2007-12-12

申请号：EP07252322.8

申请日：2007-06-08

申请人： Institut Pasteur Korea

发明人： Emans, Neil ,  Altschuler, Yoram c/o Institut Pasteur Korea ,  Kwon, Yongjun

IPC分类号： G01N33/50 ,  G01N33/74 ,  C07K14/705

CPC分类号： G01N33/5035 ,  G01N33/5008 ,  G01N33/566 ,  G01N2333/5754 ,  G01N2333/70571 ,  G01N2333/726 ,  G01N2800/12 ,  G01N2800/32

摘要： A method for identifying a compound that modifies the cellular distribution of a GPCR in a population of cells comprising:
a) taking a population of cells expressing a GPCR
b) incubating said cells with a candidate compound and with a ligand of the GPCR
c) determining distribution of said GPCR in cells treated according to step b) and comparing with distribution of GPCR in cells incubated with a ligand of the GPCR in the absence of the candidate compound;

wherein altered distribution in cells incubated with the candidate compound is indicative that the candidate compound modifies GPCR cellular distribution.

摘要翻译： 一种用于鉴定修饰细胞群中GPCR的细胞分布的化合物的方法，包括：a）取表达GPCR的细胞群b）用候选化合物和GPCR的配体将所述细胞孵育c）确定 所述GPCR在根据步骤b）处理的细胞中的分布并与不存在候选化合物时与GPCR配体孵育的细胞中GPCR的分布进行比较; 其中与候选化合物孵育的细胞中分布改变指示候选化合物修饰GPCR细胞分布。

公开(公告)号：EP2280069A1

公开(公告)日：2011-02-02

申请号：EP10012449.4

申请日：2006-09-20

申请人： Institut Pasteur Korea ,  Institut Pasteur

发明人： Emans, Neil ,  Nehrbass, Ulf

IPC分类号： C12N15/10

CPC分类号： C12N15/1086 ,  C12Q1/6897

摘要： The present invention relates to a method of detecting and/or quantifying expression of a target protein candidate in a cell, and to a method of identifying a target protein of a small molecule modulator.

摘要翻译： 本发明涉及检测和/或定量细胞中靶蛋白候选的表达的方法，以及鉴定小分子调节剂的靶蛋白的方法。