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1.发明公开DOUBLE STRAND COMPOSITIONS COMPRISING DIFFERENTIALLY MODIFIED STRANDS FOR USE IN GENE MODULATION 审中-公开包含差异修饰的STRANDS的双链组合物用于基因调节
公开(公告)号：EP1765416A2
公开(公告)日：2007-03-28
申请号：EP05757632.4
申请日：2005-06-02
发明人： BHAT, Balkrishen , PRAKASH, Thazha, P. , DANDE, Prasad , ALLERSON, Charles , GRIFFEY, Richard, H. , SWAYZE, Eric, E.
CPC分类号： C12N15/113 , C07H21/02 , C12N15/111 , C12N2310/14 , C12N2310/315 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/3231 , C12N2310/341 , C12N2310/346 , C12N2320/30 , C12N2320/51 , C12N2310/3521
摘要： The present invention provides double stranded compositions wherein each strand is modified to have a motif defined by positioning of β-D-ribonucleosides and sugar modified nucleosides. More particularly, the present compositions comprise one strand having an alternating motif and another strand having a hemimer motif, a blockmer motif, a fully modified motif or a positionally modified motif. At least one of the strands has complementarity to a nucleic acid target. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes. In preferred embodiments the compositions of the present invention hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. The present invention also provides methods for modulating gene expression.
摘要翻译： 本发明提供了双链组合物，其中每条链被修饰成具有通过定位β-D-核糖核苷和糖修饰的核苷确定的基序。 更具体地说，本发明的组合物包含具有交替基序的一条链和具有半色体基序，封闭剂基序，完全修饰的基序或位置修饰的基序的另一条链。 至少一条链与核酸靶具有互补性。 该组合物可用于靶向选定的核酸分子并调节一种或多种基因的表达。 在优选的实施方案中，本发明的组合物与靶RNA的一部分杂交，导致靶RNA的正常功能丧失。 本发明还提供了调节基因表达的方法。
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公开(公告)号：EP1765074A2
公开(公告)日：2007-03-28
申请号：EP04754153.7
申请日：2004-06-03
发明人： BHAT, Balkrishen , SWAYZE, Eric , PRAKASH, Thazha, P. , ALLERSON, Charles , DANDE, Prasad , GRIFFEY, Richard, H.
IPC分类号： A01N43/04
CPC分类号： C12N15/113 , C12N15/111 , C12N15/1137 , C12N2310/14 , C12N2310/315 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/3231 , C12N2320/50 , C12Y301/03048 , C12N2310/3525 , C12N2310/3521
摘要： The present invention provides oligomeric compounds having sufficient complementarity to hybridize to a nucleic acid target and methods for their use in modulating gene expression. In one embodiment the oligomeric compounds comprise double stranded constructs wherein one of the strands capable of hybridizing to a nucleic acid target, and has a plurality of modified ribofuranosyl nucleosides at defined locations. The presense of modifications at such defined positions greatly enhances the properties of the corresponding compositions.
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3.发明公开METHODS FOR RAPID FORENSIC ANALYSIS OF MITOCHONDRIAL DNA AND CHARACTERIZATIONG OF MITOCHONDRIAL DNA HETEROPLASMY 有权方法DNA HETEROPLASMIEMITOCHONDRIALER快速法医分析线粒体DNA与表征
公开(公告)号：EP1573065A2
公开(公告)日：2005-09-14
申请号：EP03810055.8
申请日：2003-12-05
发明人： ECKER, David, J. , CROOKE, Stanley, T. , BLYN, Lawrence, B. , HALL, Thomas, A. , JIANG, Yun , GRIFFEY, Richard, H. , SAMPATH, Rangarajan , HOFSTADLER, Steven, A. , MCNEIL, John , HANNIS, James, C. , WHITE, Neil , SAMANT, Vivek , ESHOO, Mark, W. , DRADER, Jared, J.
CPC分类号： C12Q1/686 , C12Q1/6806 , C12Q1/6844 , C12Q1/6858 , C12Q1/6872 , C12Q1/6888 , C12Q1/689 , C12Q2600/156 , G01N2333/28 , G01N2333/32 , G01N2333/35 , G01N2333/36 , C12Q2563/167 , C12Q2545/101 , C12Q2531/113 , C12Q2565/627 , C12Q2545/113
摘要： The present invention provides methods for rapid forensic analysis of mitochondrial DNA and methods for characterizing heteroplasmy of mitochondrial DNA, which can be used to assess the progression of mitochondrial diseases.
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公开(公告)号：EP1766052A1
公开(公告)日：2007-03-28
申请号：EP04754188.3
申请日：2004-06-03
发明人： BHAT, Balkrishen , SWAYZE, Eric , ALLERSON, Charles , DANDE, Prasad , PRAKASH, Thazha, P. , GRIFFEY, Richard, H.
CPC分类号： C12N15/113 , A61K31/07 , C07H21/04 , C12N15/111 , C12N2310/14 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/341 , C12N2320/51 , C12N2310/3521
摘要： The present invention provides double stranded compositions wherein the first strand comprises three different regions and the second strand is native RNA. Each region has native or modified ribofuranosyl sugar moieties that are different than those of the other two regions. At least a portion of the first oligomeric compound is complementary to and hybridizes to a nucleic acid target. The present invention also provides methods for modulating gene expression using the modified oligomeric compounds and compositions of oligomeric compounds.
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5.发明公开DOUBLE STRANDED COMPOSITIONS COMPRISING A 3’-ENDO MODIFIED STRAND FOR USE IN GENE MODULATION 审中-公开具有使用在该基因调制3'-ENDO-MODIFIED STRAND双链COMPOSITIONS
公开(公告)号：EP1644475A2
公开(公告)日：2006-04-12
申请号：EP04754155.2
申请日：2004-06-03
发明人： KRAYNACK, Brian, A. , BAKER, Brenda, F. , BHAT, Balkrishen , SWAYZE, Eric , GRIFFEY, Richard, H.
IPC分类号： C12N1/00
CPC分类号： C12N15/111 , C12N15/113 , C12N2310/315 , C12N2310/317 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2320/51
摘要： The present invention provides double stranded compositions that have a region that is complementary to a target nucleic acid. One of the targeting strand or the second strand comprises linked ribofuranosyl nucleosides and the other strand comprises linked modified nucleosides that have 3'-endo conformational geometry. The strands can be linked together or separate and may contain additional groups. The present invention also provides methods of using the compositions for modulating gene expression.
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6.发明公开COMPOSITIONS COMPRISING ALTERNATING 2'-MODIFIED NUCLEOSIDES FOR USE IN GENE MODULATION 有权COMPOSITIONS交变2'-修饰核苷包含用于USE IN基因调制
公开(公告)号：EP1560840A2
公开(公告)日：2005-08-10
申请号：EP03781743.4
申请日：2003-11-04
发明人： ALLERSON, Charles , BHAT, Balkrishen , ELDRUP, Anne, B. , MANOHARAN, Muthiah , GRIFFEY, Richard, H. , BAKER, Brenda, F. , SWAYZE, Eric, E.
CPC分类号： C07H21/00 , C07H21/04 , C12N15/111 , C12N15/113 , C12N2310/14 , C12N2310/321 , C12N2310/322 , C12N2310/323 , C12N2310/343 , C12N2310/3521 , C12N2310/3533 , C12N2320/51
摘要： The present invention provides compositions comprising at least one oligomeric compound comprising an alternating motif and further include a region that is complementary to a nucleic acid target. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes. In preferred embodiments the compositions of the present invention hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. The present invention also provides methods for modulating gene expression.
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公开(公告)号：EP1765074B1
公开(公告)日：2012-10-03
申请号：EP04754153.7
申请日：2004-06-03
发明人： BHAT, Balkrishen , SWAYZE, Eric , PRAKASH, Thazha, P. , ALLERSON, Charles , DANDE, Prasad , GRIFFEY, Richard, H.
CPC分类号： C12N15/113 , C12N15/111 , C12N15/1137 , C12N2310/14 , C12N2310/315 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/3231 , C12N2320/50 , C12Y301/03048 , C12N2310/3525 , C12N2310/3521
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公开(公告)号：EP1765415A2
公开(公告)日：2007-03-28
申请号：EP05756325.6
申请日：2005-06-02
发明人： SWAYZE, Eric, E. , BHAT, Balkrishen , GRIFFEY, Richard, H. , PRAKASH, Thazha, P. , ALLERSON, Charles
CPC分类号： C12N15/113 , C07H21/02 , C12N15/111 , C12N2310/14 , C12N2310/315 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/3231 , C12N2310/341 , C12N2310/346 , C12N2320/30 , C12N2320/51 , C12N2310/3521
摘要： The present invention provides double stranded compositions wherein one of the strands is useful in, for example, influencing the preferential loading the opposite strand into the RISC (or cleavage) complex. In particular, the present invention provides oligomeric compounds that comprise chemical modifications in at least one of the strands to drive loading of the opposite strand into the RISC (or cleavage) complex. Such modifications can be used to increase potency of duplex constructs that have been modified to enhance stability. Examples of chemical modifications that drive loading of the second strand include, but are not limited to, MOE (2'-O(CH2)2OCH3), 2'-O-methyl, -ethyl, -propyl, and -N-methylacetamide. Such modifications can be distributed throughout the strand, or placed at the 5' and/or 3' ends to make a gapmer motif on the sense strand. The activity of the 4'-thio gapmer RNA antisense strand can be improved by incorporating alternating MOE or MOE gapmer motif into the sense strand.
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9.发明公开OLIGOMERIC COMPOUNDS AND COMPOSITIONS FOR USE IN MODULATION OF SMALL NON-CODING RNAS 审中-公开低聚物化合物和组合物应用于小型非KODIENDEREN RNA的调制
公开(公告)号：EP1648914A2
公开(公告)日：2006-04-26
申请号：EP04780181.6
申请日：2004-07-30
发明人： ESAU, Christine , LOLLO, Bridget , BENNETT, C., Frank , FREIER, Susan, M. , GRIFFEY, Richard, H. , BAKER, Brenda, F. , VICKERS, Timothy , MARCUSSON, Eric, G. , KOLLER, Erich , SWAYZE, Eric, E. , JAIN, Ravi , BHAT, Balkrishen , PERALTA, Eigen
CPC分类号： C12N15/113 , C12N15/111 , C12N2310/11 , C12N2310/113 , C12N2310/14 , C12N2310/141 , C12N2310/31 , C12N2310/315 , C12N2310/317 , C12N2310/3181 , C12N2310/32 , C12N2310/321 , C12N2310/3231 , C12N2310/3341 , C12N2310/341 , C12N2310/346 , C12N2310/351 , C12N2310/3515 , C12N2320/11 , C12N2320/30 , C12N2320/50 , C12N2310/3525
摘要： Compounds, compositions and methods are provided for modulating the expression and function of small non-coding RNAs. The compositions comprise oligomeric compounds, targeted to small non-coding RNAs. Methods of using these compounds for modulation of small non-coding RNAs as well as downstream targets of these RNAs and for diagnosis and treatment of disease associated with small non-coding RNAs are also provided.
摘要翻译： 化合物，组合物和方法提供了用于调节的小非编码RNA的表达和功能。 所述组合物包含低聚化合物，有针对性的小非编码RNA。 因此，提供了使用这些化合物用于小非编码RNA的调制以及合成的RNA和用于诊断和治疗与小的非编码RNA相关的疾病的下游靶的方法。
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公开(公告)号：EP1364064A2
公开(公告)日：2003-11-26
申请号：EP02709785.6
申请日：2002-03-04
发明人： ECKER, David, J. , GRIFFEY, Richard, H. , SAMPATH, Rangarajan , HOFSTADLER, Steven , MCNEIL, John
CPC分类号： C12Q1/689 , C12Q1/686 , C12Q2600/156 , C12Q2565/627
摘要： Method for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a 'base composition signature' (BCS) which is then matched against a database of base composition signatures, by which the bioagent is identified.
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