公开(公告)号：EP0671471B1

公开(公告)日：1999-04-07

申请号：EP94901001.1

申请日：1993-11-25

申请人： Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute

发明人： NISHIYAMA, Kiyoto ,  ISHIKAWA, Yuji ,  KIMACHI, Kazuhiko ,  MAEDA, Hiroaki ,  TOKIYOSHI, Sachio

IPC分类号： C12P21/00 ,  C12P21/08 ,  C12N15/85 ,  C12N15/06 ,  C12N5/20

CPC分类号： C12N15/85 ,  C07K16/00 ,  C12N2840/20 ,  C12N2840/44

摘要： A process for efficiently producing a foreign protein, in particular, a novel foreign protein suitable for the expression of a recombinant antibody with eucaryotic cells by the genetic recombination techniques, wherein a cell containing a foreign gene introduced thereinto can be cultured in a serum-free medium by using as a host cell for foreign gene expression a fused cell obtained by the fusion of mouse myeloma with a lymphoid cell and culturable in a serum-free medium.

公开(公告)号：EP0671471A1

公开(公告)日：1995-09-13

申请号：EP94901001.1

申请日：1993-11-25

申请人： JURIDICAL FOUNDATION THE CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE

发明人： NISHIYAMA, Kiyoto ,  ISHIKAWA, Yuji ,  KIMACHI, Kazuhiko ,  MAEDA, Hiroaki ,  TOKIYOSHI, Sachio

IPC分类号： C12P21/00 ,  C12P21/08 ,  C12N15/85 ,  C12N15/06 ,  C12N5/20

CPC分类号： C12N15/85 ,  C07K16/00 ,  C12N2840/20 ,  C12N2840/44

摘要： A process for efficiently producing a foreign protein, in particular, a novel foreign protein suitable for the expression of a recombinant antibody with eucaryotic cells by the genetic recombination techniques, wherein a cell containing a foreign gene introduced thereinto can be cultured in a serum-free medium by using as a host cell for foreign gene expression a fused cell obtained by the fusion of mouse myeloma with a lymphoid cell and culturable in a serum-free medium.

摘要翻译： 一种有效生产外源蛋白质的方法，特别是适用于通过遗传重组技术用真核细胞表达重组抗体的新颖外源蛋白质，其中含有外源基因的细胞可以在无血清培养 通过使用通过小鼠骨髓瘤与淋巴样细胞融合而获得的融合细胞作为外源基因表达的宿主细胞，并在无血清培养基中培养的培养基。