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    Plasmid vector, method for detecting gene promoter activity, and assay kit
    3.
    发明公开
    Plasmid vector, method for detecting gene promoter activity, and assay kit 有权
    Plasmidvektor，Verfahren zum Erkennen derGenpromoteraktivitätund Assaykit

    公开(公告)号：EP2562258A1

    公开(公告)日：2013-02-27

    申请号：EP12181691.2

    申请日：2012-08-24

    申请人： Kabushiki Kaisha Toshiba ,  Toshiba Medical Systems Corporation

    发明人： AKAHOSHI, Eiichi ,  ISHIHARA, Mitsuko

    IPC分类号： C12N15/69 ,  C12N15/85

    CPC分类号： C12N15/63 ,  C12N15/69 ,  C12N15/85 ,  C12Q1/66 ,  C12Q1/6897

    摘要： According to one embodiment, a plasmid vector (1) includes a gene promoter (10), a first gene (21), a second gene (25), an internal ribosome entry site (23), a transcription termination signal sequence (27) and a replication origin sequence (30). The first gene (21) encodes a reporter protein for visualizing the activity of the gene promoter (10) which is disposed at the downstream of the gene promoter (10). The second gene (25) is disposed at the downstream of the gene promoter (10) and encodes a replication origin-binding protein. The internal ribosome entry site (25) is disposed between the first gene (21) and the second gene (25). The transcription termination signal sequence (27) encodes a signal for terminating the transcription of the first gene (21) and the second gene (25). The replication origin sequence (27) is recognized by the replication origin-binding protein.

    摘要翻译： 根据一个实施方案，质粒载体（1）包括基因启动子（10），第一基因（21），第二基因（25），内部核糖体进入位点（23），转录终止信号序列（27） 和复制起始序列（30）。 第一个基因（21）编码报道蛋白，用于显现位于基因启动子（10）下游的基因启动子（10）的活性。 将第二基因（25）置于基因启动子（10）的下游，并编码复制起始结合蛋白。 内部核糖体进入位点（25）位于第一基因（21）和第二基因（25）之间。 转录终止信号序列（27）编码用于终止第一基因（21）和第二基因（25）的转录的信号。 复制起始序列（27）由复制起始结合蛋白识别。

    METHOD FOR DELIVERING ACTIVATORS TO CELLS
    10.
    发明公开
    METHOD FOR DELIVERING ACTIVATORS TO CELLS 审中-公开

    公开(公告)号：EP4331681A2

    公开(公告)日：2024-03-06

    申请号：EP24150035.4

    申请日：2019-03-14

    申请人： Kabushiki Kaisha Toshiba

    发明人： ISHIHARA, Mitsuko ,  AKAHOSHI, Eiichi ,  NAITO, Katsuyuki ,  NOZAKI, Emi ,  SARUWATARI, Saeko

    IPC分类号： A61P43/00

    摘要： The present embodiment provides a method for delivering activators to cells, wherein said activator containing lipid particles are brought into contact with the cells,
    where a compound represented by the formula (1):

             Q-CHR 2      (1)

    wherein
    Q is represented by the following formula (1-Q):

             R Q1 2 N-(CR Q2 2 ) q1 -NR Q1 -(CR Q2 2 ) q2 -*     (1-Q)

    wherein
    each R Q1 is independently an alkyl,
    each R Q2 is independently hydrogen or an alkyl,
    any two of R Q1 s and R Q2 s may link together to form a nitrogen-containing alicyclic ring,
    q1 is an integer of 1 to 4,
    q2 is an integer of 0 to 4, and
    the mark * indicates the position bonding to -CHR 2 ; and
    each R is independently an aliphatic group of C 12 to C 24;
    wherein said R is represented by the following formula (1-R):

             -L R1 -C(=O)-O-L R2      (1-R)

    where
    L R1 is alkylene and
    L R2 is represented by the following formula (1-R2):

             -CH 2 -CH=CH-(CH 2 ) r2 -H     (1-R2)

    where r2 is an integer of 1 to 10.