公开(公告)号：EP1336657A4

公开(公告)日：2005-07-27

申请号：EP01982863

申请日：2001-11-20

申请人： KANEKA CORP

发明人： MATSUDA HIDEYUKI ,  KAWAMUKAI MAKOTO ,  YAJIMA KAZUYOSHI ,  IKENAKA YASUHIRO

IPC分类号： C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12P7/66 ,  C12N9/12

CPC分类号： C12N9/1085 ,  C12P7/66

摘要： A process for efficiently producing coenzyme Q10 by using a microorganism with the use of a coenzyme Q10 side chain synthase gene originating in a fungus belonging to the genus Rodotorula. A DNA having a DNA sequence represented by SEQ ID NO:1, 3 or 5 or a DNA sequence derived therefrom by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having a decaprenyl diphosphate synthase activity.

公开(公告)号：EP1354957A4

公开(公告)日：2005-01-26

申请号：EP01271890

申请日：2001-12-27

申请人： KANEKA CORP

发明人： MATSUDA HIDEYUKI ,  KAWAMUKAI MAKOTO ,  YAJIMA KAZUYOSHI ,  IKENAKA YASUHIRO

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/10 ,  C12N9/12 ,  C12N15/54 ,  C12P7/66 ,  C12R1/19

CPC分类号： C12N9/1085 ,  C12P7/66 ,  C12Y205/01031

摘要： A process for efficiently producing coenzyme Q10 by using microorganisms wherein coenzyme Q10 side-chain synthesis genes originating in fungi belonging to the genera Aspergillus and Leucosporidium are used. DNA having a DNA sequence represented by SEQ ID NO:1 or 2 or a DNA sequence derived therefrom by deletion, addition, insertion or substitution of one or more bases and encoding a protein having a decaprenyl diphosphate synthase activity.

公开(公告)号：EP1070759A4

公开(公告)日：2003-01-22

申请号：EP00902071

申请日：2000-02-03

申请人： KANEKA CORP

发明人： MATSUDA HIDEYUKI ,  KAWAMUKAI MAKOTO ,  KAZUYOSHI YAJIMA ,  IKENAKA YASUHIRO ,  NISHI KENICHI ,  HASEGAWA JUNZO ,  TAKAHASHI SATOMI

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N9/12 ,  C12P7/66 ,  C12N15/52 ,  C12N9/52 ,  C12P23/00

CPC分类号： C12N9/1085 ,  C12P7/66

摘要： A gene of decaprenyl diphosphate synthase, which is the key gene participating in the biosynthesis of coenzyme Q10 was isolated from a bacterium belonging to the family Rhizobiaceae. By transferring this gene into a microorganism such as Escherichia coli and expressing therein, coenzyme Q10 can be effectively produced.

公开(公告)号：EP1391515A4

公开(公告)日：2005-05-04

申请号：EP02722768

申请日：2002-04-25

申请人： KANEKA CORP

发明人： MATSUDA HIDEYUKI ,  KAWAMUKAI MAKOTO ,  YAJIMA KAZUYOSHI

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12P7/66

CPC分类号： C12N9/1085 ,  C12P7/66 ,  C12Y205/01031

摘要： It is intended to provide a process for efficiently producing coenzyme Q10 with the use of a microorganism wherein a coenzyme Q10 side chain synthesis gene originating in a fungus belonging to the genus Bulleromyces is used. A DNA comprising the base sequence represented by SEQ ID NO:1; a DNA having a base sequence derived from the base sequence represented by SEQ ID NO:1 by deletion, addition, insertion and/or substitution of one or more bases and encoding a protein having a decaprenyl diphosphate synthase activity; or a DNA being hybridizable with the DNA comprising the base sequence represented by SEQ ID NO:1 under stringent conditions and having a decaprenyl diphosphate synthase activity.

摘要翻译： 旨在提供一种使用微生物有效生产辅酶Q10的方法，其中使用来源于属于Bulleromyces属的真菌的辅酶Q10侧链合成基因。 包含由SEQ ID NO：1表示的碱基序列的DNA; 通过缺失，添加，插入和/或取代一个或多个碱基并编码具有十丙烯二磷酸酯合酶活性的蛋白质而具有由SEQ ID NO：1所示的碱基序列衍生的碱基序列的DNA; 或可与包含SEQ ID NO：1所示碱基序列的DNA在严格条件下杂交且具有十丙烯二磷酸合酶活性的DNA。

公开(公告)号：EP1386964A4

公开(公告)日：2005-05-04

申请号：EP02769565

申请日：2002-05-10

申请人： KANEKA CORP

发明人： MATSUDA HIDEYUKI ,  KAWAMUKAI MAKOTO ,  YAJIMA KAZUYOSHI

IPC分类号： C12N1/21 ,  C12N9/10 ,  C12P7/66 ,  C12N15/09 ,  C07K14/39 ,  C07K14/47 ,  C12P21/02

CPC分类号： C12N9/1085 ,  C12P7/66

摘要： A process for producing a long-chain prenyl diphosphate synthase (in particular, decaprenyl diphosphate synthase or solanesyl diphosphate synthase) by using a gene and a protein which are required for expressing or enhancing the enzymatic activity of the long-chain prenyl diphosphate synthase originating in an eucaryote; and a process for efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular, coenzyme Q9 or coenzyme Q10) with the use of a microorganism. DNA having a base sequence represented by SEQ ID NO:1, 3 or 5, or a DNA sequence derived therefrom by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein which is capable of expressing (functionizing)or enhancing the activity of an eucaryote-origin long-chain prenyl diphosphate synthase in a host microorganism.

摘要翻译： 本发明提供使用基因和蛋白质生产长链异戊烯基二磷酸合成酶（特别是十聚癸酸二磷酸合成酶和solanesyl二磷酸合酶）的方法，所述基因和蛋白质是实现或增强真核生物长链的活性表达所必需的 异戊二烯基二磷酸合酶，以及使用微生物有效生产其侧链具有长链类异戊二烯（特别是辅酶Q9或辅酶Q10）的辅酶Q的方法。 本发明涉及具有SEQ ID NO：1,3或5所示碱基序列的DNA，以及通过缺失，添加，插入和/或取代一至多个上述碱基序列的DNA序列 碱基，编码能够（功能）或增强宿主微生物中真核生物衍生的长链异戊二烯基二磷酸合成酶的活性表达的蛋白质。

公开(公告)号：EP1123979A4

公开(公告)日：2002-03-27

申请号：EP00954944

申请日：2000-08-24

申请人： KANEKA CORP

发明人： MATSUDA HIDEYUKI ,  KAWAMUKAI MAKOTO ,  YAJIMA KAZUYOSHI ,  IKENAKA YASUHIRO ,  HASEGAWA JUNZO ,  TAKAHASHI SATOMI

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N9/12 ,  C12N15/54 ,  C12P7/66 ,  C12R1/19

CPC分类号： C12N9/1085 ,  C12P7/66

摘要： A process for microbiologically producing coenzyme Q10 at a high efficiency by using a gene of the synthesis of coenzyme Q10 side chain originating in a fungus belonging to the genus Saitoella. A DNA having the base sequence represented by SEQ ID NO:1; a DNA having a base sequence derived from the base sequence represented by SEQ ID NO:1 by deletion, addition, insertion and/or substitution of one or more bases and encoding a protein having a decaprenyl diphosphate synthase activity; and a DNA being hybridizable under stringent conditions with the DNA comprising the base sequence represented by SEQ ID NO:1 and encoding a protein having a decaprenyl diphosphate synthase activity.