公开(公告)号：EP1306438A4

公开(公告)日：2005-03-02

申请号：EP01956776

申请日：2001-08-01

申请人： KANEKA CORP

发明人： KIZAKI NORIYUKI ,  YASOHARA YOSHIHIKO ,  HASEGAWA JUNZO

IPC分类号： C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N15/53 ,  C12P17/10 ,  C12P41/00

CPC分类号： C12N9/0008 ,  C12N9/0006 ,  C12P17/10 ,  C12P41/002

摘要： Novel polypeptides capable of forming (S)-N-benzyl-3-pyrrolidinol, DNA encoding the same and a method of using the same. A polypeptide having the following physicochemical properties (1) to (5): (1) function: asymmetrically reducing N-benzyl-3-pyrrolidinone by using NADPH as a coenzyme to form (S)-N-benzyl-3-pyrodinol; (2) optimum functional pH: 4.5 to 5.5; (3) optimum functional temperature: 40 to 45°C; (4) molecular weight: about 29,000 in gel filtration analysis, about 35,000 in SDS polyacrylamide electrophoresis; and (5) inhibitor: being inhibited by divalent copper ion. A polypeptide having the amino acid sequence represented by SEQ ID NO:1, or a polypeptide having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO:1 by the substitution, insertion, deletion and/or addition of one or more amino acids and having an enzymatic activity of asymmetrically reducing N-benzyl-3-pyrrolidinone to form (S)-N-benzyl-3-pyrrolidinol.

公开(公告)号：EP0967271A4

公开(公告)日：2002-09-25

申请号：EP97937861

申请日：1997-09-01

申请人： KANEKA CORP

发明人： YASOHARA YOSHIHIKO ,  KIZAKI NORIYUKI ,  HASEGAWA JUNZO ,  WADA MASARU ,  SHIMIZU SAKAYU ,  KATAOKA MICHIHIKO ,  YAMAMOTO K ,  KAWABATA HIROSHI ,  KITA KEIKO

IPC分类号： C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N15/53 ,  C12N15/63 ,  C12P7/62 ,  C12P41/00 ,  C12R1/91

CPC分类号： C12P7/62 ,  C12N9/0006 ,  C12P41/002

摘要： An enzyme having the activity of asymmetrically reducing a carbonyl compound to an optically active alcohol, a DNA which encodes the enzyme, a plasmid having the DNA, cells transformed by the plasmid, and a process for producing optically active alcohols with the enzyme and/or the transformed cells.

公开(公告)号：EP1123979A4

公开(公告)日：2002-03-27

申请号：EP00954944

申请日：2000-08-24

申请人： KANEKA CORP

发明人： MATSUDA HIDEYUKI ,  KAWAMUKAI MAKOTO ,  YAJIMA KAZUYOSHI ,  IKENAKA YASUHIRO ,  HASEGAWA JUNZO ,  TAKAHASHI SATOMI

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N9/12 ,  C12N15/54 ,  C12P7/66 ,  C12R1/19

CPC分类号： C12N9/1085 ,  C12P7/66

摘要： A process for microbiologically producing coenzyme Q10 at a high efficiency by using a gene of the synthesis of coenzyme Q10 side chain originating in a fungus belonging to the genus Saitoella. A DNA having the base sequence represented by SEQ ID NO:1; a DNA having a base sequence derived from the base sequence represented by SEQ ID NO:1 by deletion, addition, insertion and/or substitution of one or more bases and encoding a protein having a decaprenyl diphosphate synthase activity; and a DNA being hybridizable under stringent conditions with the DNA comprising the base sequence represented by SEQ ID NO:1 and encoding a protein having a decaprenyl diphosphate synthase activity.

公开(公告)号：EP0902011A4

公开(公告)日：2002-03-20

申请号：EP98902203

申请日：1998-02-13

申请人： KANEKA CORP

发明人： YAMADA MASAHIKO ,  NAGASHIMA NOBUO ,  HASEGAWA JUNZO

IPC分类号： B01J23/44 ,  C07B53/00 ,  C07B61/00 ,  C07C227/08 ,  C07C227/18 ,  C07C229/36 ,  C07C253/30 ,  C07C255/58 ,  C07C319/20 ,  C07C323/63

CPC分类号： C07C227/18 ,  C07C227/08 ,  C07C229/36

摘要： A highly economical and efficient process for producing optically active homophenylalanine derivatives represented by general formula (IV); and intermediates therefor and a process for producing the same. The process comprises reacting a β-benzoylacrylic acid derivative represented by general formula (II) with a 1-arylethylamine derivative represented by general formula (III) and reducing the resultant η-oxo-homophenylalanine derivative represented by general formula (I).

公开(公告)号：EP1116795A4

公开(公告)日：2005-01-26

申请号：EP00942359

申请日：2000-06-28

申请人： KANEKA CORP

发明人： KAWANO SHIGERU ,  HORIKAWA MIHO ,  YASOHARA YOSHIHIKO ,  HASEGAWA JUNZO

IPC分类号： C12N9/02 ,  C12N15/53 ,  C12P17/12 ,  C12P17/18 ,  C12P41/00 ,  C12N1/21 ,  C12N15/63 ,  C12R1/11 ,  C12R1/19 ,  C12R1/72

CPC分类号： C12N9/0004 ,  C12P17/12 ,  C12P17/18 ,  C12P41/002

摘要： A process for producing optically active pyridineethanol derivatives at a high yield which comprises stereoselectively reducing acetylpyridine derivatives by treating with an enzyme or an enzyme source having an asymmetric reduction activity; the enzyme having the asymmetric reduction activity; a DNA encoding the enzyme; a recombinant vector containing the DNA; a transformant containing the recombinant vector; and a process for producing optically active pyridineethanol derivatives from optically inactive pyridineethanol derivatives with the use of the above-described enzyme or transformant.

公开(公告)号：EP0916654A4

公开(公告)日：2003-03-26

申请号：EP97933892

申请日：1997-08-01

申请人： KANEKA CORP

发明人： YASOHARA YOSHIHIKO ,  MIYAMOTO KENJI ,  KAWANO SHIGERU ,  HASEGAWA JUNZO

IPC分类号： C07C303/30 ,  C07C309/64 ,  C07C309/66 ,  C07C309/73 ,  C07C327/32 ,  C07D317/24 ,  C07B53/00 ,  C07C309/72

CPC分类号： C07C303/30 ,  C07B2200/07 ,  C07C309/64 ,  C07C309/66 ,  C07C309/73 ,  C07C327/32 ,  C07D317/24

摘要： Novel, useful and optically active 2-aralkyl-3-sulfonyloxy-1-propanols and 2-aralkyl-3-sulfonyloxypropionic acids prepared from optically active 2-aralkyl-3-acyloxy-1-propanols as the starting compound; and optically active 2-aralkyl-3-thiopropionic acids as an important intermediate for enkephalinase inhibitors. They are commercially advantageous, optically active sulfonic esters.

公开(公告)号：EP0942068A4

公开(公告)日：2000-10-11

申请号：EP97946058

申请日：1997-11-26

申请人： KANEKA CORP

发明人： YASOHARA YOSHIHIKO ,  HASEGAWA JUNZO

IPC分类号： C07D207/12 ,  C12P17/10 ,  C12P41/00 ,  C12R1/265 ,  C12R1/645 ,  C12R1/84

CPC分类号： C12P17/10

摘要： A process for preparing optically active N-benzyl-3-pyrrolidinol efficiently by reducing N-benzyl-3-pyrrolidinone stereoselectively through an enzymatic reaction. The process is one which comprises the step of treating N-benzyl-3-pyrrolidinone with cells of a microorganism, a culture thereof or a product of treatment thereof to form a reaction fluid and the step of recovering optically active N-benzyl-3-pyrrolidinol from the fluid, and wherein the microorganism is one belonging to the genus Depodascus, Debaryomyces, Cryptococcus, Pichia, Rhodosporidium, Trichosporon, Micrococcus, Komagataella, Ogataea or Zygosaccharomyces.

公开(公告)号：EP0657415A4

公开(公告)日：1995-08-23

申请号：EP94919815

申请日：1994-06-29

申请人： KANEKA CORP

发明人： MITSUDA MASARU ,  HAYASHI SHIGEO ,  HASEGAWA JUNZO ,  UEYAMA NOBORU ,  OHASHI TAKEHISA ,  SHIBASAKI MASAKATSU

IPC分类号： C07C213/02 ,  C07C215/28 ,  C07C227/02 ,  C07C229/34 ,  C07C269/02 ,  C07C271/16 ,  C07C213/00

CPC分类号： C07C269/02 ,  C07C213/02 ,  C07C215/28 ,  C07C227/02 ,  C07C229/34 ,  C07C271/16 ,  Y02P20/55

摘要： A process for producing a 3-amino-1-nitro-4-phenyl-2-butanol derivative represented by general formula (1) and a 3-amino-2-hydroxy-4-phenyl-butyric acid derivative derived therefrom. In said formula, R and R represent each independently hydrogen or an amino-protecting group. The process can industrially provide an optically active amino alcohol derivative as an intermediate for synthesizing medicines such as an immunopotentiating carcinostatic bestatin in a high yield safely, readily and highly selectively.

公开(公告)号：EP1055732A4

公开(公告)日：2003-03-12

申请号：EP99959901

申请日：1999-12-20

申请人： KANEKA CORP

发明人： TAOKA NAOAKI ,  KIZAKI NORIYUKI ,  YASOHARA YOSHIHIKO ,  INOUE KENJI ,  HASEGAWA JUNZO

IPC分类号： C12P7/22 ,  C12P41/00 ,  C12R1/72

CPC分类号： C12P7/22 ,  C12P41/002

摘要： To efficiently produce an (R)-2-hydroxy-1-phenoxypropane derivative which is useful as a drug intermediate at a high optical purity, a phenoxyacetone derivative is R-selectively reduced by using a carbonyl reductase originating in candida, or an optionally processed culture medium of a microorganism capable of producing the above enzyme.

摘要翻译： 为了高效率地制造作为光学纯度高的药物中间体有用的（R）-2-羟基-1-苯氧基丙烷衍生物，使用来源于念珠菌的羰基还原酶或任选加工的苯氧基丙酮衍生物进行R选择性还原 能够产生上述酶的微生物的培养基。

公开(公告)号：EP1070759A4

公开(公告)日：2003-01-22

申请号：EP00902071

申请日：2000-02-03

申请人： KANEKA CORP

发明人： MATSUDA HIDEYUKI ,  KAWAMUKAI MAKOTO ,  KAZUYOSHI YAJIMA ,  IKENAKA YASUHIRO ,  NISHI KENICHI ,  HASEGAWA JUNZO ,  TAKAHASHI SATOMI

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N9/12 ,  C12P7/66 ,  C12N15/52 ,  C12N9/52 ,  C12P23/00

CPC分类号： C12N9/1085 ,  C12P7/66

摘要： A gene of decaprenyl diphosphate synthase, which is the key gene participating in the biosynthesis of coenzyme Q10 was isolated from a bacterium belonging to the family Rhizobiaceae. By transferring this gene into a microorganism such as Escherichia coli and expressing therein, coenzyme Q10 can be effectively produced.