公开(公告)号：EP1783208A4

公开(公告)日：2007-11-14

申请号：EP05757788

申请日：2005-07-06

申请人： KYOWA HAKKO KOGYO KK ,  IWATA HIROO ,  SASAI YOSHIKI

发明人： IWATA HIROO ,  SASAI YOSHIKI ,  YAMAZOE HIRONORI ,  KOBORI MASATO ,  SATOH MITSUO ,  YANO KEIICHI

IPC分类号： C12N5/0793

CPC分类号： C12N5/0619 ,  C12N2500/38 ,  C12N2506/02

摘要： It is intended to provide: a method of producing nerve cells by inducing the differentiation of embryonic stem cells; a method of inducing the differentiation of embryonic stem cells into nerve cells; a medium to be used in the production method or the differentiation induction method as described above; or a method of elevating the purity of nerve cells obtained by inducing the differentiation of embryonic stem cells. Namely, a method of conveniently, selectively or economically producing nerve cells, which are applicable to the treatment of neurodegenerative diseases and the like, by inducing the differentiation of embryonic stem cells with the use of vitamin B12 or a salt thereof, and heparin, a substance having a heparin-like function or a salt thereof.

公开(公告)号：EP1452594A4

公开(公告)日：2007-01-10

申请号：EP02781768

申请日：2002-11-14

申请人： KYOWA HAKKO KOGYO KK ,  SASAI YOSHIKI ,  IWATA HIROO

发明人： SASAI YOSHIKI ,  IWATA HIROO ,  MURAKAMI YOSHINOBU ,  SATOH MITSUO ,  KOBORI MASATO ,  YANO KEIICHI

IPC分类号： A61K35/12 ,  A61K35/48 ,  A61K38/17 ,  A61P17/00 ,  A61P25/00 ,  A61P25/16 ,  A61P25/28 ,  A61P43/00 ,  C12N5/071 ,  C12N5/073 ,  C12N15/12 ,  G01N33/50 ,  G01N33/68 ,  C12N15/09 ,  A61K38/00 ,  A61K45/00 ,  C07K14/47 ,  C12N5/06 ,  C12Q1/02 ,  G01N33/15

CPC分类号： G01N33/5073 ,  A61K35/12 ,  A61K35/48 ,  A61K38/1709 ,  C12N5/0603 ,  C12N5/0619 ,  C12N2501/415 ,  C12N2501/90 ,  C12N2501/91 ,  C12N2502/13 ,  C12N2503/02 ,  C12N2506/02 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/5058 ,  G01N33/6896

摘要： A method of obtaining a solution having an activity of inducing the differentiation of embryo stem cells into ectodermal cells or ectoderm-origin cells which involves the step of culturing stroma cells with the use of a liquid medium containing a polyanion compound and then recovering the liquid medium; a solution having an activity of inducing the differentiation of embryo stem cells into ectodermal cells or ectoderm-origin cells which is obtained by using the above method; and an inducer for the differentiation of embryo stem cells into ectodermal cells or ectoderm-origin cells.

公开(公告)号：EP1788079A4

公开(公告)日：2008-08-06

申请号：EP05765782

申请日：2005-07-08

申请人： JAPAN SCIENCE & TECH AGENCY ,  KYOWA HAKKO KOGYO KK ,  KITAGAWA YASUO ,  TORII SHUHEI

发明人： SAKURADA KAZUHIRO ,  KOBORI MASATO ,  KAMEI YUZURU ,  YAMAGUCHI HIROTAKE ,  TORIYAMA KAZUHIRO ,  TAKADA TORU

IPC分类号： C12N5/0775

CPC分类号： C12N5/0667

摘要： It is intended to provide a pluripotent cell having the following properties: (1) being contained in a mixed-cell type mass obtained by enzymatically treating a tissue collected from an animal; (2) being contained a sedimented cell mass obtained by centrifuging the mixed cell-type mass as described in the above (1); (3) selectively proliferating when cultured in a liquid culture medium containing not more than 2% of serum and 1 to 100 ng/ml of fibroblast growth factor 2; and (4) by controlling culture conditions, being capable of differentiating into cells having characteristics of adipocytes, osteoblasts, chondrocytes, tenocytes, cardiomyocytes, myoblasts, neurocytes or vascular endotheliums; cells having differentiated from the above cell; and a method of conveniently acquiring the pluripotent cell in a large amount.