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    Process for producing trans-4-hydroxy-l-proline
    7.
    发明公开
    Process for producing trans-4-hydroxy-l-proline 失效
    Verfahren zur Herstellung von Trans-4-羟基-1-脯氨酸。

    公开(公告)号：EP0641862A2

    公开(公告)日：1995-03-08

    申请号：EP94113955.2

    申请日：1994-09-06

    申请人： KYOWA HAKKO KOGYO CO., LTD.

    发明人： Ozaki, Akio ,  Mori, Hideo ,  Shibasaki, Takeshi ,  Ando, Katsuhiko ,  Chiba, Shigeru

    IPC分类号： C12P13/24 ,  C12N9/02

    CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002

    摘要： Provided is a process for industrially producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium or Amycolatopsis and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. Also provided is a novel enzyme L-proline-4-hydroxylase, which is useful for the process.

    摘要翻译： 提供了工业生产反式-4-羟基-L-脯氨酸的方法，其可用作药物的原料或作为食品添加剂。 在此过程中，L-脯氨酸在衍生自属于Dactylosporangium或Amycolatopsis属的微生物的酶源存在下转化成反式-4-羟基-L-脯氨酸，并且催化L-脯氨酸羟基化为反式 -4-羟基-L-脯氨酸，二价铁离子和2-酮戊二酸，并从水性介质中收集生成的反式-4-羟基-L-脯氨酸。 还提供了一种新型的L-脯氨酸-4-羟化酶，其可用于该方法。