公开(公告)号：EP1578356A4

公开(公告)日：2007-08-15

申请号：EP03743199

申请日：2003-02-23

申请人： MERCK & CO INC

发明人： ESPESETH AMY S ,  FERRER MARC ,  FLORES OSVALDO A ,  HAZUDA DARIA J ,  INGLESE JAMES ,  MILLER MICHAEL D ,  REGISTER BRUCE ,  SHI XIAO-PING ,  SIMON ADAM J ,  ZUCK PAUL D

IPC分类号： C07K14/47 ,  C12N15/12 ,  C07H21/02 ,  C12N1/20 ,  C12N15/00 ,  C12P21/06

CPC分类号： C07K14/47 ,  C07K14/4702 ,  C07K14/4711 ,  C07K2319/00 ,  C07K2319/50 ,  G01N2500/10

摘要： The present invention provides DNA constructs, genetically engineered host cells, and methods for identifying inhibitors of amyloid precursor protein (APP) processing. The methods provide for the convenient identification, in a single assay, of inhibitors of β-secretase and Ϝ-secretase as well as other forms of APP processing. The methods rely on fusion proteins of APP and transcription factors in which APP processing releases the transcription factors, allowing the transcription factors to activate transcription of a reporter gene. Inhibitors are identified as substances that block or diminish transcription factor release from the fusion protein, thereby causing a diminution of reporter gene readout.

公开(公告)号：EP1499748A4

公开(公告)日：2005-10-12

申请号：EP03721825

申请日：2003-04-11

申请人： MERCK & CO INC

发明人： FLORES OSVALDO A ,  GROBLER JAY ,  MURRAY EDWARD M ,  ZUCK PAUL D

IPC分类号： C07K14/02 ,  C12N15/86 ,  C12Q1/34 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/50 ,  G01N33/576 ,  C07H21/02 ,  C07H21/04 ,  C12N9/00 ,  G01N33/53

CPC分类号： G01N33/502 ,  C07K14/005 ,  C12N15/86 ,  C12N2730/10122 ,  C12N2840/203 ,  C12Q1/34 ,  C12Q1/6897 ,  C12Q1/707 ,  G01N33/5008 ,  G01N33/5067 ,  G01N33/5767 ,  G01N2333/18

摘要： The present invention features assays employing a beta-lactamase reporter system, an HCV replicon enhanced cell, and/or a chimeric HCV replicon containing a 3' UTR based on the HCV-1a 3' UTR. These features can be employed alone or together, and are preferably combined together to measure HCV replicon activity and the affect of compounds on such activity