公开(公告)号：EP1606397A1

公开(公告)日：2005-12-21

申请号：EP03816398.6

申请日：2003-08-21

申请人： Merck & Co., Inc.

发明人： EMINI, Emilio, A. ,  SHIVER, John, W. ,  BETT, Andrew, J. ,  CASIMIRO, Danilo, R. ,  CHASTAIN, Michael ,  KASLOW, David, C. ,  MORSY, Manal, A.

IPC分类号： C12N7/00

CPC分类号： C12N15/86 ,  A61K2039/5256 ,  C07K14/005 ,  C12N2710/10343 ,  C12N2710/10371 ,  C12N2740/16122 ,  C12N2740/16134

摘要： Adenoviral serotypes differ in their natural tropism. The various serotypes of adenovirus have been found to differ in at least their capsid proteins (e.g., penton-base and hexon proteins), proteins responsible for cell binding (e.g, fiber proteins), and proteins involved in adenovirus replication. This difference in tropism and capsid proteins among serotypes has led to the many research efforts aimed at redirecting the adenovirus tropism by modification of the capsid proteins. The present invention bypasses such requirement for capsid protein modification as it presents a recombinant, replication-defective adenovirus of serotype 24, a rare adenoviral serotype, and methods for generating the alternative, recombinant adenovirus. Additionally, means of employing the recombinant adenovirus for the delivery and expression of exogenous genes are provided.

公开(公告)号：EP0954579A1

公开(公告)日：1999-11-10

申请号：EP97931187.0

申请日：1997-06-20

申请人： MERCK & CO., INC. ,  BAYLOR COLLEGE OF MEDICINE

发明人： MORSY, Manal, A. ,  GU, Ming, Cheng ,  ZHAO, Jing ,  CASKEY, C., Thomas ,  KOCHANET, Stefan

IPC分类号： C12N15 ,  A61K48 ,  C07K14 ,  C12N5

CPC分类号： C12N15/8509 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0362 ,  A61K48/00 ,  C07K14/5759 ,  C07K14/72 ,  C12N2799/022 ,  C12N2800/30

摘要： Gene therapy can treat obesity in mammals. An obesity regulating gene is delivered to a mammal. Preferably, the gene encodes letpin or a leptin receptor. The protein which is delivered and expressed in vivo is more effective than protein which is injected into the animal.

公开(公告)号：EP0921820A1

公开(公告)日：1999-06-16

申请号：EP97932580.0

申请日：1997-06-20

申请人： MERCK & CO., INC.

发明人： MORSY, Manal, A. ,  GU, Ming, Cheng ,  ZHAO, Jing ,  CASKEY, C., Thomas

IPC分类号： A61K48 ,  A61K38 ,  A61P3 ,  C07K14 ,  C12N15

CPC分类号： C12N15/86 ,  A61K38/2264 ,  A61K48/00 ,  C07K14/5759 ,  C07K14/715 ,  C12N2710/10343

摘要： Gene therapy can treat obesity in mammals. An obesity regulating gene is delivered to a mammal. Preferably, the gene encodes leptin or a leptin receptor. The protein which is delivered and expressed in vivo is more effective than protein which is injected into the animal.

摘要翻译： 基因疗法可以治疗哺乳动物的肥胖症。 肥胖调节基因被递送给哺乳动物。 优选地，该基因编码瘦蛋白或瘦蛋白受体。 在体内递送并表达的蛋白质比注射到动物体内的蛋白质更有效。

公开(公告)号：EP1097243A1

公开(公告)日：2001-05-09

申请号：EP99933840.3

申请日：1999-07-09

申请人： MERCK & CO., INC.

发明人： RICHARDS, Karen ,  RUSHMORE, Thomas, H. ,  MORSY, Manal, A.

IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C12N5/10 ,  C12N15/86 ,  C12N15/63

CPC分类号： C12N15/86 ,  C12N2710/10343 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/85 ,  C12Q1/6897

摘要： Adenoviral vectors are used to transfer a promoter/reporter gene construct to mammalian cell cultures. The promoter/reporter gene construct is used to determine if a candidate inducing agent has promoter-inducing activity; or can be used to determine if a candidate promoter has activity in the presence of a known inducer.

公开(公告)号：EP1084236A1

公开(公告)日：2001-03-21

申请号：EP99927250.3

申请日：1999-06-04

申请人： MERCK & CO., INC.

发明人： MORSY, Manal, A. ,  SANDIG, Volker

IPC分类号： C12N15/00 ,  C12N5/00 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K38/2264 ,  A61K48/00 ,  C07K14/5759 ,  C12N2710/10343 ,  C12N2800/30 ,  C12N2830/38

摘要： Helper dependent adenoviral vectors are suitable for use in gene therapy, as they are stable, contain only a very small amount less than about 0.5 % of helper virus contamination, and are less immunogenic than previous adenoviral vectors. The only viral DNA in the vectors are ITRs and packaging signals, and overall size is about 26-38 kb.