公开(公告)号：EP0768893B1

公开(公告)日：2003-02-26

申请号：EP95922160.7

申请日：1995-06-01

申请人： Merck & Co., Inc.

发明人： DONNELLY, John, J. ,  LIU, Margaret, A. ,  MARTINEZ, Douglas ,  MONTGOMERY, Donna, L.

IPC分类号： A61K39/12 ,  C12N15/37

CPC分类号： C12N7/00 ,  A61K48/00 ,  C07K14/005 ,  C12N2710/20022

摘要： DNA constructs encoding papilloma virus gene products, capable of being expressed upon direct introduction into animal tissues are novel prophylactic pharmaceuticals which can provide immune protection against infection by papilloma virus.

公开(公告)号：EP1791978A2

公开(公告)日：2007-06-06

申请号：EP05798108.6

申请日：2005-09-13

申请人： Merck & Co., Inc.

发明人： ANDERSON, Annaliesa, S. ,  MONTGOMERY, Donna, L.

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07H21/02 ,  C12N15/00

CPC分类号： C07K14/31 ,  A61K39/00

摘要： The present invention features polypeptides comprising an amino acid sequence structurally related to SEQ ID NO: 1 and uses of such polypeptides. SEQ ID NO: 1 is a truncated derivative of a full-length S. aureus polypeptide. The full-length polypeptide is referred to herein as full-length penicillin-binding protein 4 (“PBP4”). A His-tagged derivative of SEQ ID NO: 1 was found to produce a protective immune response against S. aureus.

摘要翻译： 本发明的特征在于包含与SEQ ID NO：1结构相关的氨基酸序列的多肽以及这些多肽的用途。 SEQ ID NO：1是全长金黄色葡萄球菌多肽的截短衍生物。 全长多肽在本文中称为全长青霉素结合蛋白4（“PBP4”）。 发现SEQ ID NO：1的His-标记的衍生物产生针对金黄色葡萄球菌的保护性免疫应答。

公开(公告)号：EP0768893A1

公开(公告)日：1997-04-23

申请号：EP95922160.0

申请日：1995-06-01

申请人： Merck & Co., Inc.

发明人： DONNELLY, John, J. ,  LIU, Margaret, A. ,  MARTINEZ, Douglas ,  MONTGOMERY, Donna, L.

IPC分类号： C12N15 ,  A61K39 ,  A61K48 ,  A61P31 ,  A61P37 ,  C07H21 ,  C07K14 ,  C12R1

CPC分类号： C12N7/00 ,  A61K48/00 ,  C07K14/005 ,  C12N2710/20022

摘要： DNA constructs encoding papilloma virus gene products, capable of being expressed upon direct introduction into animal tissues are novel prophylactic pharmaceuticals which can provide immune protection against infection by papilloma virus.

公开(公告)号：EP1651166B1

公开(公告)日：2010-02-17

申请号：EP04778847.6

申请日：2004-07-22

申请人： Merck & Co., Inc.

发明人： ANDERSON, Annaliesa, S. ,  JANSEN, Kathrin Ute ,  KELLY, Rosemarie ,  SCHULTZ, Loren, D. ,  MONTGOMERY, Donna, L. ,  MCCLEMENTS, William, L.

IPC分类号： A61K39/085 ,  C07K14/31 ,  C12N15/31 ,  C12N15/81

CPC分类号： A61K39/085 ,  C07K14/31 ,  C07K2319/35

公开(公告)号：EP0746611B1

公开(公告)日：2003-04-16

申请号：EP93915201.3

申请日：1993-06-02

申请人： Merck & Co., Inc. ,  University of Kent at Canterbury

发明人： TUITE, Michael F. ,  FREEDMAN, Robert B. ,  SCHULTZ, Loren, D. ,  ELLIS, Ronald, W. ,  MARKUS, Henry, Z. ,  MONTGOMERY, Donna, L.

IPC分类号： C12N15/12 ,  C12N15/61 ,  C12N15/81 ,  C12N9/90 ,  C12N1/19 ,  C07K14/815

CPC分类号： C12N9/90 ,  C07K14/811 ,  C07K14/815 ,  C12N15/81

摘要： Disclosed is a process for increasing the yield of disulfide bonded recombinant proteins produced by yeast, especially recombinant secreted proteins. The enzyme protein disulfide isomerase (PDI) catalyzes the formation of disulfide bonds in secretory and cell-surface proteins. We disclose the construction of recombinant strains of the yeast Saccharomyces cerevisiae which overproduce either human PDI or yeast PDI in a regulated fashion. These strains show greatly increased secretion of disulfide bonded proteins of potential therapeutic significance. These strains have the potential to increase the production of various disulfide bonded proteins.

公开(公告)号：EP2126070A2

公开(公告)日：2009-12-02

申请号：EP08726829.8

申请日：2008-03-14

申请人： Merck & Co., Inc.

发明人： ANDERSON, Annaliesa, S. ,  MCNEELY, Tessie ,  COOK, James, C., III ,  MCCLEMENTS, William, L. ,  MONTGOMERY, Donna, L.

IPC分类号： C12N15/00 ,  C12N1/20 ,  C12Q1/68 ,  C07K1/00 ,  C07H21/04

CPC分类号： A61K39/085

摘要： The present invention features polypeptides comprising an amino acid sequence structurally related to SEQ ID NO: 1 and uses of such polypeptides. SEQ ID NO: 1 is a truncated derivative of a full-length S. epidermidis polypeptide. The full-length naturally occurring polypeptide is referred to herein as full-length ORF2695e. A His-tagged derivative of SEQ ID NO: 1 was found to produce a protective immune response against S. epidermidis.

摘要翻译： 本发明的特征在于包含与SEQ ID NO：1结构相关的氨基酸序列的多肽以及这些多肽的用途。 SEQ ID NO：1是全长表皮葡萄球菌多肽的截短衍生物。 全长天然存在的多肽在本文中被称为全长ORF2695e。 发现SEQ ID NO：1的His-标记的衍生物产生针对表皮葡萄球菌的保护性免疫应答。

公开(公告)号：EP2117574A2

公开(公告)日：2009-11-18

申请号：EP08794276.9

申请日：2008-01-18

申请人： Merck & Co., Inc.

发明人： ANDERSON, Annaliesa, S. ,  MCNEELY, Tessie ,  COOK, James, C., III ,  MCCLEMENTS, William, L. ,  MONTGOMERY, Donna, L.

IPC分类号： A61K38/00

CPC分类号： A61K39/085 ,  A61K2039/55505

摘要： The present invention features polypeptides comprising an amino acid sequence structurally related to SEQ ID NO: 1 and uses of such polypeptides. SEQ ID NO: 1 is a truncated derivative of a full-length S. epidermidis polypeptide. The full-length naturally occurring polypeptide is referred to herein as full-length ORF1319e. A His-tagged derivative of SEQ ID NO: 1 was found to produce a protective immune response against S. epidermidis.

公开(公告)号：EP1651166A2

公开(公告)日：2006-05-03

申请号：EP04778847.6

申请日：2004-07-22

申请人： Merck & Co., Inc.

发明人： ANDERSON, Annaliesa, S. ,  JANSEN, Kathrin Ute ,  KELLY, Rosemarie ,  SCHULTZ, Loren, D. ,  MONTGOMERY, Donna, L. ,  MCCLEMENTS, William, L.

IPC分类号： A61K6/00

CPC分类号： A61K39/085 ,  C07K14/31 ,  C07K2319/35

摘要： The present invention features polypeptides comprising an amino acid sequence structurally related to SEQ ID NO: 1, uses of such polypeptides, and expression systems for producing such polypeptides. SEQ ID NO: 1 is a truncated derivative of a full length S. aureus polypeptide. The full-length polypeptide is referred to herein as full-length “ORF0657n”. Polypeptides containing the amino acid sequence of SEQ ID NO: 1 were found to produce a protective immune response against S. aureus.

摘要翻译： 本发明的特征在于包含在结构上与SEQ ID NO：1有关的氨基酸序列的多肽，这种多肽的用途以及用于产生这种多肽的表达系统。 SEQ ID NO：1是全长金黄色葡萄球菌多肽的截短衍生物。 全长多肽在本文中称为全长“ORF0657n”。 发现含有SEQ ID NO：1的氨基酸序列的多肽产生针对金黄色葡萄球菌的保护性免疫应答。

公开(公告)号：EP0746611A1

公开(公告)日：1996-12-11

申请号：EP93915201.0

申请日：1993-06-02

申请人： Merck & Co., Inc. ,  THE UNIVERSITY OF KENT AT CANTERBURY

发明人： TUITE, Michael F. ,  FREEDMAN, Robert B. ,  SCHULTZ, Loren, D. ,  ELLIS, Ronald, W. ,  MARKUS, Henry, Z. ,  MONTGOMERY, Donna, L.

IPC分类号： C12N15 ,  C07K14 ,  C12N1 ,  C12N9 ,  C12P21 ,  C12R1

CPC分类号： C12N9/90 ,  C07K14/811 ,  C07K14/815 ,  C12N15/81

摘要： Disclosed is a process for increasing the yield of disulfide bonded recombinant proteins produced by yeast, especially recombinant secreted proteins. The enzyme protein disulfide isomerase (PDI) catalyzes the formation of disulfide bonds in secretory and cell-surface proteins. We disclose the construction of recombinant strains of the yeast Saccharomyces cerevisiae which overproduce either human PDI or yeast PDI in a regulated fashion. These strains show greatly increased secretion of disulfide bonded proteins of potential therapeutic significance. These strains have the potential to increase the production of various disulfide bonded proteins.