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公开(公告)号：EP1436397A2
公开(公告)日：2004-07-14
申请号：EP02773740.2
申请日：2002-10-10
发明人： EMINI, Emilio, A. , KASLOW, David, C. , BETT, Andrew, J. , SHIVER, John, W. , NICOSIA, Alfredo , LAHM, Armin , LUZZAGO, Alessandra , CORTESE, Riccardo , COLLOCA, Stefano
CPC分类号： C07K14/005 , A61K2039/5256 , A61K2039/53 , A61K2039/545 , A61K2039/57 , C12N15/86 , C12N2710/10343 , C12N2770/24222 , C12N2770/24234 , C12N2800/108 , C12N2810/6018 , C12N2830/003 , C12N2840/20 , C12N2840/203
摘要： The present invention features Ad6 vectors and a nucleic acid encoding a Met-NS3-NS4A-NS4B-NS5A-NS5B polypeptide containing an inactive NS5B RNA-dependent RNA polymerase region. The nucleic acid is particularly useful as a component of an adenovector or DNA plasmid vaccine providing a broad range of antigens for generating an HCV specific cell mediated immune (CMI) response against HCV.
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公开(公告)号：EP1711518B1
公开(公告)日：2009-11-18
申请号：EP05701091.0
申请日：2005-01-18
发明人： CIRILLO, Agostino , COLLOCA, Stefano , ERCOLE, Bruno, Bruni , MEOLA, Annalisa , NICOSIA, Alfredo , SPORENO, Elisabetta
IPC分类号： C07K14/075 , C12N15/861 , A61K48/00
CPC分类号： C12N15/86 , A61K38/00 , A61K45/06 , A61K2039/5256 , C07K14/005 , C07K14/70503 , C12N7/00 , C12N2710/10321 , C12N2710/10343 , C12N2710/10351 , C12N2710/10362 , C12N2740/16134 , C12N2770/24222 , C12N2770/24234 , C12N2810/60 , Y02A50/386 , Y02A50/394 , Y02A50/41 , Y02A50/412
摘要： The present invention provides recombinant replication-defective adenoviral vectors derived from chimpanzee adenoviruses and methods for generating recombinant adenoviruses in human E1-expressing cell lines. The invention also provides compositions and methods suitable for use for the delivery and expression of transgenes encoding immunogens against which a boosted immune response is desired. The invention further provides methods of generating clinical grade vector stocks suitable for use in humans. In a particular embodiment the invention contemplates the use of vectors comprising transgenes which encode tumor associated antigens in vaccines and pharmaceutical compositions for the prevention and treatment of cancer.
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3.发明公开ADENOVIRAL AMPLICON AND PRODUCER CELLS FOR THE PRODUCTION OF REPLICATION-DEFECTIVE ADENOVIRAL VECTORS, METHODS OF PREPARATION AND USE THEREOF 审中-公开腺病毒的扩增子与生产细胞用于生产复制缺陷型腺病毒载体，方法为生产和使用该
公开(公告)号：EP1809753A1
公开(公告)日：2007-07-25
申请号：EP05801950.6
申请日：2005-10-27
IPC分类号： C12N15/861
CPC分类号： C12N15/86 , C12N7/00 , C12N2710/10343 , C12N2710/10352 , C12N2800/108 , C12N2820/60 , C12N2830/006
摘要： The present invention relates to a plasmid that can be used for the development of efficient producer cell lines for the production of helper independent adenovirus vectors carrying multiple deletions of non-structural as well as structural genes. More specifically, the present invention provides producer cells which comprise a novel adenoviral amplicon that can be used to complement a multi-deleted adenoviral vectors and obtain high titer preparations. The amplicon is an episomal plasmid that expresses Ad5 E2 viral genes (i.e.,polymerase, pre-terminal protein and DNA binding protein) and E4 orf6 , the EBV the latent origin of replication (OriP) as well as adenoviral origins of replications in form of a covalent junction of left and right ITRs. This plasmid is capable of self-replication upon induction of Ad5 E2 gene expression. The invention further includes methods for the preparation of the disclosed producer cells and uses of the cells to produce viral vectors on a scale that is sufficient for therapeutic uses.
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公开(公告)号：EP1711518A2
公开(公告)日：2006-10-18
申请号：EP05701091.0
申请日：2005-01-18
发明人： CIRILLO, Agostino , COLLOCA, Stefano , ERCOLE, Bruno, Bruni , MEOLA, Annalisa , NICOSIA, Alfredo , SPORENO, Elisabetta
IPC分类号： C07K14/075 , C12N15/861 , A61K48/00
CPC分类号： C12N15/86 , A61K38/00 , A61K45/06 , A61K2039/5256 , C07K14/005 , C07K14/70503 , C12N7/00 , C12N2710/10321 , C12N2710/10343 , C12N2710/10351 , C12N2710/10362 , C12N2740/16134 , C12N2770/24222 , C12N2770/24234 , C12N2810/60 , Y02A50/386 , Y02A50/394 , Y02A50/41 , Y02A50/412
摘要： The present invention provides recombinant replication-defective adenoviral vectors derived from chimpanzee adenoviruses and methods for generating recombinant adenoviruses in human E1-expressing cell lines. The invention also provides compositions and methods suitable for use for the delivery and expression of transgenes encoding immunogens against which a boosted immune response is desired. The invention further provides methods of generating clinical grade vector stocks suitable for use in humans. In a particular embodiment the invention contemplates the use of vectors comprising transgenes which encode tumor associated antigens in vaccines and pharmaceutical compositions for the prevention and treatment of cancer.
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