公开(公告)号：EP2497493A1

公开(公告)日：2012-09-12

申请号：EP10826901.0

申请日：2010-11-01

申请人： Mie University ,  Biocomo Co., Ltd. ,  National Institute of Biomedical Innovation

发明人： YASUTOMI, Yasuhiro ,  KAWANO, Mitsuo ,  NOSAKA, Tetsuya ,  FUKUMURA, Masayuki

IPC分类号： A61K39/04 ,  A61K35/76 ,  A61K39/00 ,  A61K48/00 ,  A61P31/06 ,  C12N15/09

CPC分类号： A61K35/76 ,  A61K39/04 ,  A61K2039/543 ,  C12N2760/18743

摘要： Disclosed is a intranasal spray-type tuberculosis vaccine, which has a high prophylactic effect on human tuberculosis, particularly adult tuberculosis. The nebulizable tuberculosis vaccine for intranasal administration comprises a paramyxovirus gene (particularly rhPIV2) having, integrated therein, a gene encoding an α antigen derived from an acid-fast bacterium (e.g., an α antigen derived from Mycobacterium kansasii or Mycobacterium bovis BCG), an analogue of the gene, or a variant of the gene which has an equivalent function to that of the gene.

摘要翻译： 公开了鼻内喷雾型结核疫苗，其对人类结核病特别是成人结核病具有高预防效果。 用于鼻内给药的可喷雾结核疫苗包括其中整合有编码来自抗酸细菌的±抗原的基因（例如，源自堪萨斯分枝杆菌或牛分枝杆菌BCG的±抗原）的副粘病毒基因（特别是rhPIV2），一种 基因的类似物，或与该基因具有等同功能的基因的变体。

公开(公告)号：EP2674491B1

公开(公告)日：2017-06-14

申请号：EP12744339.8

申请日：2012-02-08

申请人： Mie University ,  BioComo Inc.

发明人： FUKUMURA, Masayuki ,  KAWANO, Mitsuo ,  NOSAKA, Tetsuya ,  OHTSUKA, Junpei

IPC分类号： C12N15/09 ,  A61K35/76 ,  A61P37/04 ,  C12N5/10

CPC分类号： C12N15/64 ,  A61K48/00 ,  C12N7/00 ,  C12N2760/18622 ,  C12N2760/18643 ,  C12N2760/18651 ,  C12N2760/18652

公开(公告)号：EP2674491A1

公开(公告)日：2013-12-18

申请号：EP12744339.8

申请日：2012-02-08

申请人： Mie University ,  BioComo Inc.

发明人： FUKUMURA, Masayuki ,  KAWANO, Mitsuo ,  NOSAKA, Tetsuya ,  OHTSUKA, Junpei

IPC分类号： C12N15/09 ,  A61K35/76 ,  A61P37/04 ,  C12N5/10

CPC分类号： C12N15/64 ,  A61K48/00 ,  C12N7/00 ,  C12N2760/18622 ,  C12N2760/18643 ,  C12N2760/18651 ,  C12N2760/18652

摘要： The present invention discloses a cell system, as a host cell to be infected with an F gene-deficient virus, which can constitutively and stably express the F protein, and a method for producing an F gene-deficient virus by utilizing the cell. A non-proliferative human parainfluenza type 2 virus vector is produced by co-culturing an F gene-deficient human parainfluenza type 2 virus with a Vero cell having the F gene of human parainfluenza type 2 virus in such a manner that the F gene is non-inducibly expressed, and isolating viral particles from a culture supernatant.

公开(公告)号：EP3636755A1

公开(公告)日：2020-04-15

申请号：EP19196282.8

申请日：2013-12-25

申请人： BioComo Inc. ,  Mie University

发明人： FUKUMURA, Masayuki ,  OHTSUKA, Junpei ,  NOSAKA, Tetsuya ,  TSURUDOME, Masato ,  KAWANO, Mitsuo ,  HARA, Ken-ichiro

IPC分类号： C12N15/09 ,  A61K39/145 ,  A61P31/16 ,  C12N7/02 ,  C12N7/04 ,  A61K48/00 ,  A61P35/00 ,  A61K39/155 ,  A61P31/14

摘要： Disclosed are: a virus vector in which a gene encoding an antigenic polypeptide is integrated in human parainfluenza virus type 2 gene, wherein the antigenic polypeptide is expressed in the form of a fusion protein with a viral structural protein; and a method for producing the same. The virus vector of the present invention contains a quantitatively large amount of the antigenic peptide on the virus particle and can efficiently deliver the antigenic polypeptide to a target cell.

公开(公告)号：EP3459424A1

公开(公告)日：2019-03-27

申请号：EP17799490.2

申请日：2017-05-18

申请人： Mie University

发明人： MIZOGUCHI, Akira ,  TANAKA, Koji ,  KATAYAMA, Naoyuki ,  NOSAKA, Tetsuya ,  TANAKA, Kyosuke ,  WANG, Shujie ,  KAITO, Aika ,  SAI, Kousyoku ,  KIMURA, Kazushi

IPC分类号： A61B1/00 ,  G01N1/30 ,  G01N21/64 ,  G01N21/78 ,  G01N33/48

摘要： A cancer test device (1) is provided with: an application unit (40) for applying a staining agent (45), which can selectively stain a product of a cancer-relating gene in a living cell a chromatic color, onto a group of living cells; an imaging unit (10) for imaging the group of living cells having the staining agent (45) applied thereto; and a determination unit (52) for determining the level of malignancy of cancerization of the group of living cells on the basis of the state of the stained expression pattern of the cancer-relating gene in the group of living cells in an image obtained by the aforementioned imaging.

公开(公告)号：EP3309251A1

公开(公告)日：2018-04-18

申请号：EP16807639.6

申请日：2016-06-13

申请人： Mie University ,  BioComo Inc.

发明人： NOSAKA, Tetsuya ,  TSURUDOME, Masato ,  FUKUMURA, Masayuki ,  OHTSUKA, Junpei ,  YUDA, Masao ,  IWANAGA, Shiroh

IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K39/015 ,  A61K39/02 ,  A61K39/04 ,  A61K39/12 ,  A61P31/04 ,  A61P31/12 ,  A61P35/00 ,  C07K14/115

CPC分类号： A61K39/155 ,  A61K39/00 ,  A61K39/015 ,  A61K39/02 ,  A61K39/04 ,  A61K39/12 ,  C07K14/115 ,  C12N7/00 ,  C12N15/09 ,  C12N15/86 ,  Y02A50/386 ,  Y02A50/39 ,  Y02A50/392 ,  Y02A50/394 ,  Y02A50/412

摘要： The present invention relates to: a virus vector, which can effectively transfer a macromolecular antigenic peptide into target cells while maintaining a three-dimensional structure that is required for functioning as an antigen; and a vaccine utilizing the vector. Specifically, disclosed are: a virus vector, in which a nucleic acid encoding an antigenic polypeptide is integrated immediately 5' upstream of HN gene of an F gene-defective Paramyxoviridae virus gene, wherein the antigenic polypeptide is expressed as a fusion protein of 130 or more amino acid residues, fused with a TM sequence and/or a CT sequence derived from the virus.

摘要翻译： 本发明涉及一种病毒载体，其能够维持作为抗原发挥功能所需要的三维结构，并且能够有效地将大分子抗原肽转移到靶细胞中， 和利用该载体的疫苗。 具体而言，公开了一种病毒载体，其中编码抗原性多肽的核酸立即整合到F基因缺陷型副粘病毒科病毒基因的HN基因的5'上游，其中该抗原性多肽表达为融合蛋白130或 与TM序列融合的更多氨基酸残基和/或来自病毒的CT序列。

公开(公告)号：EP2940134A1

公开(公告)日：2015-11-04

申请号：EP13868750.4

申请日：2013-12-25

申请人： BioComo Inc. ,  Mie University

发明人： FUKUMURA, Masayuki ,  OHTSUKA, Junpei ,  NOSAKA, Tetsuya ,  TSURUDOME, Masato ,  KAWANO, Mitsuo ,  HARA, Ken-ichiro

IPC分类号： C12N15/09 ,  A61K39/145 ,  A61P31/16 ,  C12N7/02 ,  C12N7/04 ,  A61K48/00 ,  A61P35/00

CPC分类号： C12N15/86 ,  A61K39/0011 ,  A61K39/12 ,  A61K39/145 ,  A61K39/155 ,  A61K2039/5252 ,  A61K2039/5256 ,  C07K2319/00 ,  C12N7/00 ,  C12N15/09 ,  C12N2760/16034 ,  C12N2760/18734 ,  C12N2760/18743 ,  C12N2760/18762 ,  C12N2760/18763 ,  Y02A50/386 ,  Y02A50/394 ,  Y02A50/397

摘要： Disclosed are: a virus vector in which a gene encoding an antigenic polypeptide is integrated in human parainfluenza virus type 2 gene, wherein the antigenic polypeptide is expressed in the form of a fusion protein with a viral structural protein; and a method for producing the same. The virus vector of the present invention contains a quantitatively large amount of the antigenic peptide on the virus particle and can efficiently deliver the antigenic polypeptide to a target cell.

摘要翻译： 公开的是：将编码抗原多肽的基因整合到人类副流感病毒2型基因中的病毒载体，其中抗原多肽以具有病毒结构蛋白的融合蛋白的形式表达; 及其制造方法。 本发明的病毒载体含有定量大量的病毒颗粒上的抗原肽，并可有效地将抗原多肽递送至靶细胞。