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1.发明授权
公开(公告)号：EP1635949B1
公开(公告)日：2010-12-29
申请号：EP04776709.0
申请日：2004-06-16
发明人： MARTINELL, Brian , CALABOTTA, Beth, J. , HEINZEN, Richard, J. , KLEMM, Richard, F. , MCCABE, Dennis, E. , ROBERTS, Gail, A. , SMITH, Lori, A.
摘要： A process of mechanical separation of embryos from seeds for genetic transplantation employs counter-rotating cylinders together with one or more culling, hydration, separation, and viability testing steps to provide high throughput of viable, transplantable tissue.
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2.发明公开
公开(公告)号：EP1404850A2
公开(公告)日：2004-04-07
申请号：EP02742392.0
申请日：2002-07-05
发明人： BARTON, Kenneth , CROW, Laura , GILBERTSON, Larry , HUANG, Yong , MARTINELL, Brian , PETERSEN, Michael, W. , PILLER, Kenneth, J. , WANG, Qi
CPC分类号： C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
摘要： The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprise transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
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3.发明公开
公开(公告)号：EP1635949A1
公开(公告)日：2006-03-22
申请号：EP04776709.0
申请日：2004-06-16
发明人： MARTINELL, Brian , CALABOTTA, Beth, J. , HEINZEN, Richard, J. , KLEMM, Richard, F. , MCCABE, Dennis, E. , ROBERTS, Gail, A. , SMITH, Lori, A.
摘要： A process of mechanical separation of embryos from seeds for genetic transplantation employs counter-rotating cylinders together with one or more culling, hydration, separation, and viability testing steps to provide high throughput of viable, transplantable tissue.
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公开(公告)号：EP4059342A1
公开(公告)日：2022-09-21
申请号：EP22168297.4
申请日：2011-12-16
摘要： The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.
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5.发明授权
公开(公告)号：EP1404850B1
公开(公告)日：2011-10-26
申请号：EP02742392.0
申请日：2002-07-05
发明人： BARTON, Kenneth , CROW, Laura , GILBERTSON, Larry , HUANG, Yong , MARTINELL, Brian , PETERSEN, Michael, W. , PILLER, Kenneth, J. , WANG, Qi
CPC分类号： C12N15/8205 , C12N15/8209 , C12N15/8263 , C12N15/8265 , C12N15/8289
摘要： The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprise transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
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