公开(公告)号：EP2824186A4

公开(公告)日：2015-11-25

申请号：EP14748825

申请日：2014-01-07

申请人： NINGXIA EPPEN BIOTECH CO LTD

发明人： MA JIYIN ,  WEN TINGYI ,  CHEN JINLONG ,  LIANG YONG ,  LIU SHUWEN ,  WEI AIYING ,  YANG LIPENG ,  REN RUI ,  MENG GANG ,  ZHAO CHUNGUANG ,  ZHANG YUN ,  SHANG XIULING ,  GUO XIAOWEI

IPC分类号： C12P13/08 ,  C12N1/21 ,  C12N15/61 ,  C12N15/70

CPC分类号： C12P13/08 ,  C12N9/88 ,  C12N15/70 ,  C12Y402/01003

摘要： Provided is a method of producing L-lysine by fermentation, which comprises the steps of modifying an aconitase gene and/or regulatory element thereof in a chromosome of a bacterium so that the activity and/or the expression amount of the aconitase of the bacterium are reduced but not eliminated; and producing L-lysine by the fermentation of the modified bacterium. In addition, also provided are methods and uses derived from the method as well as bacteria used in these methods and uses.

摘要翻译： 提供通过发酵生产L-赖氨酸的方法，其包括在细菌染色体中修饰乌头酸酶基因和/或调节元件的步骤，使得细菌的乌头酸酶的活性和/或表达量为 减少但不消除; 并通过改良细菌的发酵生产L-赖氨酸。 此外，还提供了从该方法得到的方法和用途以及这些方法和用途中使用的细菌。