公开(公告)号：EP1709163A4

公开(公告)日：2007-10-24

申请号：EP05722431

申请日：2005-01-14

申请人： NOVOZYMES INC

发明人： VLASENKO ELENA ,  CHERRY JOEL ,  XU FENG

IPC分类号： C12N9/00 ,  C07H21/04 ,  C11D3/00 ,  C11D7/42 ,  C12P7/06 ,  C12P7/18 ,  C12P7/36 ,  C12P7/40 ,  C12P13/04 ,  C12P19/04 ,  C12P19/14

CPC分类号： C12P19/14 ,  C12P7/06 ,  C12P13/04 ,  Y02E50/17 ,  Y02E50/343

摘要： The present invention relates to methods for degrading a lignocellulosic material, comprising: treating the lignocellulosic material with an effective amount of one or more. cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant. The present invention also relates to methods for producing an organic, substance, comprising: (a) saccharifying a lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one such surfactant; (b) fermenting the saccharified lignocellulosic material of step (a) with one or more fermentating microoganisms; and (c) recovering the organic substance from the fermentation.

公开(公告)号：EP1499733A4

公开(公告)日：2005-09-14

申请号：EP03724363

申请日：2003-04-22

申请人： NOVOZYMES INC

发明人： BRODY HOWARD ,  OTANI SUZANNE ,  CHERRY JOEL

IPC分类号： C12N15/10 ,  C12N15/80

CPC分类号： C12N15/1082 ,  C12N15/102 ,  C12N15/1034

摘要： The present invention relates to methods for preparing nucleotide variants, comprising: introducing into a population of filamentous fungal host cells: (i) circular plasmids comprising a DNA sequence and a plasmid replicator mediating autonomous replication, wherein the circularized plasmids are linearized by digestion of the DNA sequence and removal of a portion of the DNA sequence; and (ii) a library of DNA fragments comprising a mutation(s) of the DNA sequence, wherein the fragments comprise at least two regions, one region(s) which is homologous to the 5' region or the 3' region of the gap in the linearized DNA and/or plasmid sequences and a second region(s) which are homologous to the 5' region or the 3' region of the DNA fragments; wherein the linearized plasmids and the DNA fragments recombine by in vivo recombination to produce autonomously replicating plasmids comprising variants of the DNA sequence; isolating the autonomously replicating plasmids.

摘要翻译： 本发明涉及制备核苷酸变体的方法，包括：向丝状真菌宿主细胞群中引入：（i）包含DNA序列和介导自主复制的质粒复制子的环状质粒，其中通过消化所述环化质粒 DNA序列和去除部分DNA序列; 和（ii）包含DNA序列突变的DNA片段文库，其中所述片段包含至少两个区域，与gap的5'区域或3'区域同源的一个或多个区域 在线性化的DNA和/或质粒序列中以及与DNA片段的5'区域或3'区域同源的第二区域; 其中所述线性化质粒和所述DNA片段通过体内重组重组以产生包含所述DNA序列的变体的自主复制质粒; 分离自主复制质粒。

公开(公告)号：EP1660637A4

公开(公告)日：2009-10-21

申请号：EP04809617

申请日：2004-08-25

申请人： NOVOZYMES INC

发明人： TETER SARAH ,  CHERRY JOEL ,  WARD CONNIE ,  HARRIS PAUL ,  JONES AUBREY ,  YI JUNG

IPC分类号： C12N9/42 ,  C12N15/56 ,  C12P19/14 ,  C12S11/00

CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  C12Y302/01091 ,  C12Y302/01176

摘要： The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.

公开(公告)号：EP1735454A4

公开(公告)日：2008-05-07

申请号：EP05736399

申请日：2005-03-10

申请人： NOVOZYMES INC

发明人： BERKA RANDY ,  CHERRY JOEL

IPC分类号： C12P19/04 ,  C07G99/00 ,  C12P5/00 ,  C12P5/02 ,  C12P7/00 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/26 ,  C12P7/28 ,  C12P7/40 ,  C12P13/04 ,  C12P17/04 ,  C12P19/02 ,  C13B35/00

CPC分类号： C12P19/14 ,  C12P5/023 ,  C12P7/00 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/26 ,  C12P7/28 ,  C12P7/40 ,  C12P13/04 ,  C12P17/04 ,  C12P19/02 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

摘要： The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microorganisms, and (c) recovering the organic substance from the fermentation.