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公开(公告)号：EP2316938A1
公开(公告)日：2011-05-04
申请号：EP09804765.7
申请日：2009-08-07
申请人： National Institute of Advanced Industrial Science and Technology , THE HOKUREN FEDERATION OF AGRICULTURAL COOPERATIVES , National University Corporation Hokkaido University
IPC分类号： C12N15/09
CPC分类号： C12N15/8203 , C12N15/86 , C12N2770/14043
摘要： The present invention is to provide a novel non-diffusing plant virus vector wherein virus vector infection and proliferation are possible only in a recombinant plant transfected with a gene necessary for viral proliferation, thereby enabling avoidance of unintended diffusion of a recombinant virus, a selective and specific expression system therefor, and a method of expression thereof which comprises combining a non-diffusing virus vector lacking a gene involved in intercellular movement of a cucumber mosaic virus (CMV) genome and a transgenic plant transfected with the lacked gene involved in intercellular movement for the non-diffusing virus vector to establish infection and proliferation selectively and specifically in the transgenic plant.
摘要翻译： 本发明提供一种新的非扩散性植物病毒载体，其中病毒载体的感染和增殖仅在用病毒增殖所必需的基因转染的重组植物中是可能的，从而能够避免重组病毒的非预期扩散，选择性和 其特异性表达系统及其表达方法，其包括将缺少参与黄瓜花叶病毒（CMV）基因组的细胞间运动的基因的非扩散病毒载体与转染了缺乏的涉及细胞间移动的基因的转基因植物 非扩散病毒载体在转基因植物中选择性和特异性地建立感染和增殖。
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公开(公告)号：EP2316938B1
公开(公告)日：2016-06-22
申请号：EP09804765.7
申请日：2009-08-07
申请人： National Institute of Advanced Industrial Science and Technology , THE HOKUREN FEDERATION OF AGRICULTURAL COOPERATIVES , National University Corporation Hokkaido University
CPC分类号： C12N15/8203 , C12N15/86 , C12N2770/14043
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3.发明公开NUCLEIC ACID MOLECULE FOR EXPRESSING FOREIGN GENE IN PLANT AND METHOD THEREFOR 审中-公开富兰克林大学杜克大学生物科学与技术研究所（NUKLEINSÄUREMOLEKÜLZUR EXPRESSION FREMDER GENE IN PFLANZEN UND VERFAHRENDAFÜR
公开(公告)号：EP2960331A1
公开(公告)日：2015-12-30
申请号：EP14754539.6
申请日：2014-02-20
申请人： National Institute of Advanced Industrial Science and Technology , National University Corporation Hokkaido University
CPC分类号： C12N15/8257 , C12N15/8203 , C12N15/8206 , C12N15/8207
摘要： A nucleic acid molecule for expressing a foreign gene in a plant, said nucleic acid molecule carrying, in this order, a right border (RB) sequence derived from the T-DNA sequence of an agrobacterium, an expression promoter sequence capable of functioning in the aforesaid plant, a sequence corresponding to the RNA2 genome of cucumber mosaic virus wherein a 2b protein-encoding gene has been partly or entirely substituted by the aforesaid foreign gene, and a left border (LB) sequence derived from the T-DNA sequence of an agrobacterium.
摘要翻译： 一种用于在植物中表达外源基因的核酸分子，所述核酸分子依次携带衍生自农杆菌的T-DNA序列的右边界（RB）序列，能够在其中起作用的表达启动子序列 上述植物，对应于黄瓜花叶病毒的RNA2基因组的序列，其中2b蛋白编码基因已经被上述外源基因部分或完全取代，以及衍生自以上所述外源基因的T-DNA序列的左边界（LB）序列 农杆菌。
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公开(公告)号：EP3828274A1
公开(公告)日：2021-06-02
申请号：EP19841320.5
申请日：2019-07-25
发明人： MASUTA, Chikara , INUKAI, Tsuyoshi , MATSUNAGA, Wataru , ISODA, Reika , MATSUMURA, Takeshi , ATSUMI, Go
IPC分类号： C12N15/113 , A01H3/00 , C12N5/10 , C12N15/82 , C12P1/00
摘要： Plants are created with desired traits by conveniently and rapidly inhibiting methylation of target DNA in plants, without using recombination technology. Scaffold RNA produced by transcription of target DNA is cleaved in an RNA-directed DNA methylation mechanism.
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公开(公告)号：EP4083207A1
公开(公告)日：2022-11-02
申请号：EP20904326.4
申请日：2020-12-25
发明人： ATSUMI, Go , MATSUMURA, Takeshi , MASUTA, Chikara , INUKAI, Tsuyoshi , MATSUNAGA, Wataru , ISODA, Reika
IPC分类号： C12N15/113 , A01H1/00
摘要： According to the present invention, methylation of a target DNA in a plant is suppressed quickly and easily without using recombinant technology, and a plant having desired traits is thereby created. In an RNA-directed DNA methylation mechanism, binding between scaffold RNA produced by transcription of the target DNA and an siRNA-AGO4 complex is inhibited.
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公开(公告)号：EP3760037A1
公开(公告)日：2021-01-06
申请号：EP19748249.0
申请日：2019-01-31
摘要： An object of the present invention is to provide a cucumber mosaic virus (CMV)-resistant solanaceous plant. The present invention provides a CMV-resistant solanaceous plant having a mutated eIF4E gene encoding an eIF4E protein nonfunctional for CMV. In addition, the present invention provides a method for producing a CMV-resistant plant, the method comprising mutating an eIF4E gene of a solanaceous plant into a mutated eIF4E gene encoding an eIF4E protein nonfunctional for CMV.
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公开(公告)号：EP2478768A1
公开(公告)日：2012-07-25
申请号：EP10815411.3
申请日：2010-09-09
摘要： The present invention provides an anti-plant virus agent which exhibits preventative and therapeutic effects on plant virus diseases. The anti-plant virus agent of the present invention is characterized by containing at least one compound selected from the group consisting of ascorbic acid derivatives represented by Formula (I) (wherein, R 1 to R 4 each independently represents a hydrogen atom, -SO 3 H -PO 3 H 2 , a glycosyl group, or -COR 11 , wherein R 11 represents an unsubstituted or substituted C1-30 alkyl group, or an unsubstituted or substituted C2-30 alkenyl group, with the proviso that R 1 to R 4 may not all be hydrogen atoms at the same time), and salts thereof.
摘要翻译： 本发明提供对植物病毒病具有预防和治疗作用的抗植物病毒剂。 本发明的抗植物病毒剂的特征在于含有至少一种选自由式（I）表示的抗坏血酸衍生物的化合物（其中，R 1〜R 4各自独立地表示氢原子，-SO 3 H -PO 3 H 2，糖基或-COR 11，其中R 11表示未取代或取代的C 1-30烷基，或未取代或取代的C 2-30烯基，条件是R 1至R 4可能不全部同时为氢原子）及其盐。
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