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    METHOD FOR MAKING ACTIVATED CARBOXYPEPTIDASES
    2.
    发明公开
    METHOD FOR MAKING ACTIVATED CARBOXYPEPTIDASES 有权
    用于生产活性炭羧

    公开(公告)号：EP2084179A2

    公开(公告)日：2009-08-05

    申请号：EP07847255.2

    申请日：2007-11-21

    申请人： Novo Nordisk A/S

    发明人： NØRGAARD, Per

    IPC分类号： C07K14/37

    CPC分类号： C12N9/48 ,  C07K14/62 ,  C07K2319/50 ,  C12N15/80

    摘要： The invention is related to a method for making an activated carboxypeptidase in a fungi cell comprising introducing a DNA sequence encoding a proform of the carboxypeptidase wherein a Kex2 site has been introduced in the prosequence of the carboxypeptidase, culturing the fungi cell under conditions suitable for expression of the procarboxypeptidase and cleaving off the prosequence within the cell to liberate the free active form of the carboxypeptidase. The invention is also related to methods for making mature human insulin and human insulin analogues by use of the activated carboxypeptidase enzyme.

    MATING FACTOR ALPHA PRO-PEPTIDE VARIANTS
    3.
    发明公开
    MATING FACTOR ALPHA PRO-PEPTIDE VARIANTS 审中-公开
    PAARUNG VON FAKTOR-ALPHA-PROPEPTID-VARIANTEN

    公开(公告)号：EP3110834A1

    公开(公告)日：2017-01-04

    申请号：EP15708175.3

    申请日：2015-03-02

    申请人： Novo Nordisk A/S

    发明人： NØRGAARD, Per

    IPC分类号： C07K14/435 ,  C12N15/00

    CPC分类号： C07K14/395 ,  C07K14/605 ,  C07K2319/00 ,  C12P21/02

    摘要： The present invention is related to Mating Factor α pro-peptide variants useful for the recombinant expression of polypeptides comprising a GLP-1 peptide in yeasts. The invention is also related to DNA sequences, vectors and host cells for use in expressing polypeptides in yeasts.

    摘要翻译： 本发明涉及可用于在酵母中重组表达包含GLP-1肽的多肽的交配因子α前肽变体。 本发明还涉及用于在酵母中表达多肽的DNA序列，载体和宿主细胞。

    METHOD FOR MAKING POLYPEPTIDES
    6.
    发明公开
    METHOD FOR MAKING POLYPEPTIDES 有权
    制备多肽的方法

    公开(公告)号：EP2504355A1

    公开(公告)日：2012-10-03

    申请号：EP10782288.4

    申请日：2010-11-25

    申请人： Novo Nordisk A/S

    发明人： ANDERSEN, Asser, Sloth ,  LAUTRUP-LARSEN, Inger ,  NØRGAARD, Per

    IPC分类号： C07K14/47 ,  C12N15/81 ,  C12N9/48 ,  C12P21/06 ,  C12N9/60 ,  C12P21/02

    CPC分类号： C07K14/575 ,  C12N9/60 ,  C12N15/81 ,  C12P21/02

    摘要： The invention provides an improved method for producing polypeptides with a C-terminal glycine in a yeast transformant being characterized in having a non functional
    KEX1 gene. The method is in particular well suited to produce polypeptides with an aromatic amino acid residue attached to the C-terminal glycine. The yeast strain may have further non-functional protease genes selected from
    PEP4, YPS1, MKCl, YPS3, YPS5, YPS6, YPS7, PRB1, STE13 and
    KEX2 .

    摘要翻译： 本发明提供了在酵母转化体中产生具有C端甘氨酸的多肽的改进方法，其特征在于具有非功能性KEX1基因。 该方法特别适合于生产具有连接至C端甘氨酸的芳族氨基酸残基的多肽。 酵母菌株可具有选自PEP4，YPS1，MKCl，YPS3，YPS5，YPS6，YPS7，PRB1，STE13和KEX2的其他非功能性蛋白酶基因。

    METHOD FOR MAKING MATURATED INSULIN POLYPEPTIDES
    10.
    发明公开
    METHOD FOR MAKING MATURATED INSULIN POLYPEPTIDES 有权
    用于生产成熟INSULINPOLYPEPTIDE

    公开(公告)号：EP2069502A1

    公开(公告)日：2009-06-17

    申请号：EP07820604.2

    申请日：2007-09-26

    申请人： NOVO NORDISK A/S

    发明人： NØRGAARD, Per ,  SLOTH ANDERSEN, Asser

    IPC分类号： C12N15/62 ,  C07K14/62

    CPC分类号： C07K14/62

    摘要： The invention is related to a method for making mature human insulin or an analogue thereof by culturing a fungi cell comprising a DNA sequence encoding a precursor for human insulin or an analogue of human insulin which precursor comprises the B-chain of human insulin or an analogue thereof, the A-chain of human insulin or an analogue thereof and a C-peptide linking the B-chain and the A-chain together thereof, wherein the C-peptide comprises at least one Kex2 cleavage site and an amino acid sequence attached at one end to the C-terminal amino acid residue in the B-chain and at the other end to the Kex2 site which amino acid sequence will facilitate a more efficient Kex2 cleavage within the fungi cell. The C-terminal extension of the B-chain may furthermore be capable of subsequently being cleaved off from the C-terminal amino acid residue in the B-chain by means of a carboxypeptidase activity either within the fungi cell or subsequently in the culture medium.