A METHOD OF SCREENING FOR PROTEIN SECRETING RECOMBINANT HOST CELLS
    7.
    发明授权
    A METHOD OF SCREENING FOR PROTEIN SECRETING RECOMBINANT HOST CELLS 有权
    VERFAHREN ZUM SCREENING AUF蛋白质酶联免疫吸附剂WIRTSZELLEN

    公开(公告)号:EP1675947B1

    公开(公告)日:2009-01-07

    申请号:EP04762918.3

    申请日:2004-10-13

    申请人: Novozymes A/S

    发明人: HOFF, Tine

    IPC分类号: C12N15/10 C12N15/75 C12Q1/68

    CPC分类号: C12N15/75 C12N15/1051

    摘要: The invention describes a method of screening for protein secreting recombinant host cells comprising screening for promoter activity of a stress inducible promoter. The method can be used for rapid identification of actively secreting transformants and can be used to screen recombinant libraries for transformants secreting proteins.

    摘要翻译: 本发明描述了筛选蛋白质分泌重组宿主细胞的方法,包括筛选应激诱导型启动子的启动子活性。 该方法可用于快速鉴定活性分泌型转化体,可用于筛选分泌蛋白质转化体的重组文库。

    POLYPEPTIDES HAVING SERINE PROTEASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME AND THEIR APPLICATION IN ANIMAL FEED
    9.
    发明公开
    POLYPEPTIDES HAVING SERINE PROTEASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME AND THEIR APPLICATION IN ANIMAL FEED 审中-公开
    具有丝氨酸蛋白酶活性的多肽和编码多核苷酸及其在动物饲料中的应用

    公开(公告)号:EP3215615A1

    公开(公告)日:2017-09-13

    申请号:EP15790110.9

    申请日:2015-11-03

    申请人: Novozymes A/S

    IPC分类号: C12N9/52 C12N9/64

    摘要: The present invention relates to animal feed or animal feed additives comprising polypeptides having protease activity and uses thereof. Specifically, the proteases are serine S1 proteases from Janibacter, Terracoccus and Knoellia, all belonging to the family Intrasporangiaceae of the suborder Micrococcineae. The proteases have a high activity at a broad pH-range (pH 3-7) and are thus highly active during the entire passage through the digestive tract. It also relates to the methods for producing the proteases and for using the proteases to improve animal performance and the nutritional value of animal feed.

    摘要翻译: 本发明涉及包含具有蛋白酶活性的多肽的动物饲料或动物饲料添加剂及其用途。 具体而言,蛋白酶是来自Janibacter,Terracoccus和Knoellia的丝氨酸S1蛋白酶,全部属于亚目微球菌科的胞内孢子科。 蛋白酶在广泛的pH范围(pH 3-7)具有高活性,因此在通过消化道的整个过程中高度活跃。 它还涉及生产蛋白酶和使用蛋白酶改善动物性能和动物饲料营养价值的方法。