公开(公告)号：EP1639102A2

公开(公告)日：2006-03-29

申请号：EP04738924.2

申请日：2004-06-18

申请人： Novozymes A/S ,  Novozymes North America, Inc. ,  CHR. HANSEN A/S

发明人： PATKAR, Shamkant, Anant ,  HIGGINS, Don ,  FATUM, Tine, Muxoll ,  VIND, Jesper ,  MADKOR, Sabry ,  SØRENSEN, Thomas, Lykke

IPC分类号： C12N9/20 ,  C12N15/55 ,  C11D3/386 ,  A23C19/04

CPC分类号： A21D8/042 ,  C12N9/18 ,  C12N9/20 ,  C12Y301/01032

摘要： The inventors have used protein engineering to develop variants of fungal phospholipases. Starting from a parent phospholipase, they have modified the amino acid sequence to arrive at variants which have phospholipase activity (generally, at roughly the same level as the parent enzyme) and have a lower lipase activity on triglycerides than the parent enzyme.

摘要翻译： 发明人已经使用蛋白质工程来开发真菌磷脂酶的变体。 从亲本磷脂酶开始，它们修饰了氨基酸序列以得到具有磷脂酶活性的变体（通常，与亲本酶大致相同的水平），并且对甘油三酯具有比亲本酶更低的脂肪酶活性。

公开(公告)号：EP1799819A2

公开(公告)日：2007-06-27

申请号：EP05816037.5

申请日：2005-09-30

申请人： Novozymes Inc. ,  Novozymes A/S

发明人： OTANI, Suzanne ,  YAVER, Debbie ,  GE, Haiyan ,  LIN, Janine ,  AMOLO, Christopher ,  BORCH, Kim ,  PATKAR, Shamkant, Anant ,  LAMSA, Michael ,  CHERRY, Barbara

IPC分类号： C12N9/20

CPC分类号： C12N9/20 ,  C12Y301/01003

摘要： The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

公开(公告)号：EP0531372B2

公开(公告)日：2004-04-14

申请号：EP91909971.3

申请日：1991-05-08

申请人： Novozymes A/S

发明人： RASMUSSEN, Grethe ,  MIKKELSEN, Jan, Möller ,  SCHÜLEIN, Martin ,  PATKAR, Shamkant, Anant ,  HAGEN, Fred ,  HJORT, Carsten, Mailand ,  HASTRUP, Sven

IPC分类号： C12N9/42 ,  C12N15/56 ,  C11D3/386

CPC分类号： C12Q1/6895 ,  C11D3/38645 ,  C12N9/2437 ,  C12N15/80 ,  C12Y302/01004 ,  C12Y302/01039 ,  C12Y302/01091 ,  D06M16/003 ,  D06P5/137 ,  D06P5/158 ,  D21H17/005 ,  D21H21/10

摘要： The present invention relates to cellulase preparations consisting essentially of a homogeneous endoglucanase component. The cellulase preparation may be employed in the treatment of cellulose-containing fabrics for harshness reduction, for color clarification, or to provide a localized variation in the color of such fabrics, or in the treatment of paper pulp.

公开(公告)号：EP1404827A2

公开(公告)日：2004-04-07

申请号：EP02701241.8

申请日：2002-02-25

申请人： Novozymes A/S

发明人： TSUTSUMI, Noriko ,  VIND, Jesper ,  PATKAR, Shamkant, Anant

IPC分类号： C12N9/20 ,  C12N15/10

CPC分类号： C11D3/386 ,  A21D8/042 ,  C11D3/38627 ,  C12N9/20 ,  C12N15/1027

摘要： The inventors have isolated novel genes with a high homology to the T. lanuginosus lipase gene and are thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The expressed proteins may further be screened to identify lipolytic enzymes with improved properties. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity).

公开(公告)号：EP1309674A1

公开(公告)日：2003-05-14

申请号：EP01960183.0

申请日：2001-07-19

申请人： Novozymes A/S

发明人： STERGAARD, Peter, Rahbek ,  HJORT, Carsten, M. ,  DEUSSEN, Heinz-Josef ,  ZUDEL, Magali ,  EBDRUP, S ren ,  CHRISTENSEN, S ren ,  PATKAR, Shamkant, Anant

IPC分类号： C12N9/16 ,  C07K14/38 ,  C12P41/00 ,  C07B57/00 ,  C12P7/42 ,  C07C51/09

CPC分类号： C12N9/16 ,  C12N9/18 ,  C12P7/40 ,  C12P41/005

摘要： An esterase isolated from Aspergillus oryzae is capable of stereoselective hydrolysis of chiral esters and also has arylesterase activity (EC 3.1.1.2) and feruloyl esterase activity (EC 3.1.1.73). The esterase has only a limited homology to known amino acid sequences.

公开(公告)号：EP1799819B1

公开(公告)日：2011-03-23

申请号：EP05816037.5

申请日：2005-09-30

申请人： Novozymes Inc. ,  Novozymes A/S

发明人： OTANI, Suzanne ,  YAVER, Debbie ,  GE, Haiyan ,  LIN, Janine ,  AMOLO, Christopher ,  BORCH, Kim ,  PATKAR, Shamkant, Anant ,  LAMSA, Michael ,  CHERRY, Barbara

IPC分类号： C12N9/20

CPC分类号： C12N9/20 ,  C12Y301/01003

摘要： The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

公开(公告)号：EP1054956B1

公开(公告)日：2006-05-31

申请号：EP99934304.9

申请日：1999-02-17

申请人： Novozymes A/S

发明人： BORCH, Kim ,  VIND, Jesper ,  SVENDSEN, Allan ,  PETERSEN, Dorte, Aaby ,  PATKAR, Shamkant, Anant ,  BOJSEN, Kirsten

IPC分类号： C12N9/20 ,  C11D3/386

CPC分类号： C11D1/83 ,  C11D1/02 ,  C11D3/38627 ,  C12N9/20

摘要： Variants of Lipolase® (wild-type Humicola lanuginosa lipase) with a certain distribution of electrically charged amino acids have a particularly good first-wash performance in a detergent solution with a high ratio of anionic to non-ionic surfactant. The effect is achieved by attaching a positively charged peptide extension at the N-terminal and by imposing certain restrictions on the charge distribution in the region corresponding to amino acid positions 90-101 and at position 210. The inventors further devised a method of developing variants with such performance from Lipolase by attaching a peptide extension at the N-terminal and substituting amino acids in the region 90-101 or in the immediate surroundings in the three-dimensional structure. The lipases may further provide additional benefits, such as whiteness maintenance and dingy cleanup.

公开(公告)号：EP1360278A2

公开(公告)日：2003-11-12

申请号：EP02710765.5

申请日：2002-02-07

申请人： Novozymes A/S

发明人： MUNK, Signe ,  VIND, Jesper ,  BORCH, Kim ,  PATKAR, Shamkant, Anant ,  GLAD, Sanne, O. Schroder ,  SVENDSEN, Allan

IPC分类号： C12N9/00 ,  C11D3/00

CPC分类号： C11D3/38627

摘要： Attaching a peptide extension to the C-terminal amino acid of a lipase re-duces the tendency to form odor. This may lead to lipase variants with a reduced odor generation when washing textile soiled with fat which includes relatively short-chain fatty acyl groups (e.g. up to C8) such as dairy stains containing butter fat or tropical oils such as coconut oil or palm kernel oil.

公开(公告)号：EP2264157B1

公开(公告)日：2014-05-14

申请号：EP10182266.6

申请日：2007-11-26

申请人： Novozymes A/S

发明人： DE MARIA, Leonardo ,  BRASK, Jesper ,  SKJØT, Michael ,  PATKAR, Shamkant, Anant ,  BORCH, Kim ,  SVENDSEN, Allan

IPC分类号： C12N9/20 ,  C12P7/40

CPC分类号： C12N9/20 ,  C12P7/40 ,  C12P7/62 ,  C12P7/6418 ,  C12Y301/01003

公开(公告)号：EP1360278B1

公开(公告)日：2009-09-23

申请号：EP02710765.5

申请日：2002-02-07

申请人： Novozymes A/S

发明人： MUNK, Signe ,  VIND, Jesper ,  BORCH, Kim ,  PATKAR, Shamkant, Anant ,  GLAD, Sanne, O. Schrøder ,  SVENDSEN, Allan

IPC分类号： C12N9/00 ,  C11D3/00

CPC分类号： C11D3/38627

摘要： Attaching a peptide extension to the C-terminal amino acid of a lipase re-duces the tendency to form odor. This may lead to lipase variants with a reduced odor generation when washing textile soiled with fat which includes relatively short-chain fatty acyl groups (e.g. up to C8) such as dairy stains containing butter fat or tropical oils such as coconut oil or palm kernel oil.