公开(公告)号：EP2814953A4

公开(公告)日：2015-10-28

申请号：EP13749460

申请日：2013-02-14

申请人： PACIFIC BIOSCIENCES CALIFORNIA

发明人： BJORNSON KEITH ,  HANES JEREMIAH ,  MILLER ERIK ,  KAMTEKAR SATWIK ,  SEBO LUBOMIR ,  BROGLEY LOUIS

IPC分类号： C07H19/00 ,  C07K14/36 ,  C12M1/38 ,  C12N9/10 ,  C12N15/54 ,  C12Q1/68

CPC分类号： G01N33/582 ,  C07H19/06 ,  C07H19/16 ,  C07H21/04 ,  C07K14/36 ,  C12N9/10 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2523/313 ,  C12Q2535/113 ,  C12Q2563/107 ,  C12Q2563/131 ,  G01N33/583

摘要： Labelled nucleotide analogs comprising protein shields for improving enzyme photostability in single molecule real time sequencing. Nucleotide analogs of the invention have a protein shield based on bis-biotin and avidin between the dye moieties and nucleotide moieties of the analog. The protein prevents the direct interaction of the dye moiety with the enzyme carrying out nucleotide synthesis preventing photodamage to the enzyme. The nucleotide analogs of the invention can have multiple dyes and multiple nucleotide moieties.

公开(公告)号：EP3008094A4

公开(公告)日：2017-04-12

申请号：EP14810199

申请日：2014-06-12

申请人： PACIFIC BIOSCIENCES CALIFORNIA INC

发明人： MILLER ERIK ,  KAMTEKAR SATWIK ,  BJORNSON KEITH ,  HANES JEREMIAH

IPC分类号： C07K19/00 ,  G01N33/68

CPC分类号： G01N33/58 ,  C07K14/36

摘要： Multi-biotinylated reactants are provided which can be used in divalent complexes for various applications such as colocalization, labeling, immobilization, and purification. Methods for constructing, purifying, and using the bis-biotinylated reactants are also provided. In certain embodiments, two bis-biotinylated reactants are bound to a single streptavidin tetramer to provide a complex having a 1:1 stoichiometry with respect to the bis-biotinylated reactants.

摘要翻译： 提供多生物素化反应物，其可以用于二价复合物用于各种应用，例如共定位，标记，固定和纯化。 还提供了构建，纯化和使用双生物素化反应物的方法。 在某些实施方案中，两种双生物素化的反应物与单个链霉抗生物素蛋白四聚体结合以提供相对于双生物素化反应物具有1：1化学计量的复合物。