公开(公告)号：EP2814953A4

公开(公告)日：2015-10-28

申请号：EP13749460

申请日：2013-02-14

申请人： PACIFIC BIOSCIENCES CALIFORNIA

发明人： BJORNSON KEITH ,  HANES JEREMIAH ,  MILLER ERIK ,  KAMTEKAR SATWIK ,  SEBO LUBOMIR ,  BROGLEY LOUIS

IPC分类号： C07H19/00 ,  C07K14/36 ,  C12M1/38 ,  C12N9/10 ,  C12N15/54 ,  C12Q1/68

CPC分类号： G01N33/582 ,  C07H19/06 ,  C07H19/16 ,  C07H21/04 ,  C07K14/36 ,  C12N9/10 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2523/313 ,  C12Q2535/113 ,  C12Q2563/107 ,  C12Q2563/131 ,  G01N33/583

摘要： Labelled nucleotide analogs comprising protein shields for improving enzyme photostability in single molecule real time sequencing. Nucleotide analogs of the invention have a protein shield based on bis-biotin and avidin between the dye moieties and nucleotide moieties of the analog. The protein prevents the direct interaction of the dye moiety with the enzyme carrying out nucleotide synthesis preventing photodamage to the enzyme. The nucleotide analogs of the invention can have multiple dyes and multiple nucleotide moieties.

公开(公告)号：EP3030683A4

公开(公告)日：2017-05-03

申请号：EP14834734

申请日：2014-08-05

申请人： PACIFIC BIOSCIENCES CALIFORNIA INC

发明人： SEBO LUBOMIR ,  HANES JEREMIAH ,  SHEN GENE ,  BROGLEY LOUIS ,  YUE STEPHEN ,  ZHENG FRANK ,  LAPIN YURI ,  LYLE JOHN ,  OSUNA HONEY ,  FEDOROV ANDREI

IPC分类号： C12Q1/68 ,  C07F9/6558 ,  C07F9/6561 ,  C07H19/10 ,  G01N21/64 ,  G01N23/223

CPC分类号： C07H19/10 ,  C07F9/65586 ,  C07F9/65616

摘要： Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.

摘要翻译： 提供受保护的荧光试剂化合物及其合成方法。 这些化合物可用于各种基于荧光的分析方法，包括高密度分析大量高度多重光学反应，如单分子实时核酸测序反应。 这些化合物含有荧光染料元素，可以在理想的波长下以高灵敏度检测化合物，允许化合物特异性地被目标生物分子识别的结合元件和保护性屏蔽元​​件，其减少荧光染料元素之间的不希望的接触 和结合的目标生物分子，并因此减少了荧光染料元件对结合的目标生物分子的光损伤。