摘要:
The invention provides isolated Hm2 nucleic acids, and their encoded proteins. The present invention provides methods and compositions relating to altering Hm2 concentration and/or composition of plants. The invention further provides expression cassettes, host cells, transgenic plants, and antibody compositions. Also, the invention provides methods of identifying plant transformation by survival of transformed plant cells or tissues on a cyclic tetrapeptide toxin. The invention further provides methods of imparting disease resistance to plants susceptible to fungal pathogens, which utilize cyclic tetrapeptide toxins.
摘要:
The HM1 gene in maize confers race-specific resistance to the pathogen, Cochliobolus carbonum. We have used transposon mutagenesis to tag, clone, and characterize several HM1 alleles. The gene can be used as a selectable marker in conjunction with the toxin produced by C. carbonum.
摘要:
Compositions and methods for enhancing disease resistance to a pathogen in a plant are provided. Methods of the invention comprise stably transforming a plant with an antisense nucleotide sequence for a gene encoding an enzyme in the C-5 porphyrin metabolic pathway and operably linking the antisense sequence to a pathogen-inducible promoter, such that invasion of a cell by a pathogen elicits a hypersensitive-like response that results in confinement of the pathogen to cells of initial contact. Transformed plants and seeds are provided. Nucleotide sequences encoding a wild-type maize urod gene useful in the present invention and the amino acid sequence for the protein encoded thereby are provided. These compositions are also useful for regulating cell death in specifically targeted tissues. A maize lesion mimic, dominant mutant phenotype, designated Les22, and the molecular basis for its manifestation are also provided.
摘要:
The present invention is drawn to methods and compositions for suppressing cell death in plants. Specifically, novel proteins and genes are provided for use in plant transformation. The proteins and genes are useful for activating disease resistance, enhancing plant cell transformation efficiency, engineering herbicide resistance, genetically targeting cell ablations, and other methods involving the regulation of cell death in plants.
摘要:
A process is described in which PCR-based cDNA libraries with anchored ends are made, a PCR--based cDNA subtracted (PCSUB) library is produced therefrom, and the ends of a cDNA clone are isolated, via PCR, from an anchored-ends library.
摘要:
The H^_M^_1^_ gene in maize confers race-specific resistance to the pathogen, Cochliobolus carbonum. We have used transposon mutagenesis to tag, clone, and characterize several H^_M^_1^_ alleles. The gene can be used as a selectable marker in conjunction with the toxin produced by C. carbonum.
摘要:
A methodology for ascertaining gene function entails selection of mutations in androgenetic haploids which are produced by fertilizing a maize plant carrying the indeterminate gametophyte gene (ig) with pollen obtained from a mutagenized plant. Genes that control quantitative characters can be identified, for example, by fertilizing a first inbred carrying the ig gene with pollen from a second inbred that has been mutagenized. Changes in the phenotype of the hybrid progeny then are identified and characterized. A method for direct selection of androgenetic haploids is provided.
摘要:
The H^_M^_1^_ gene in maize confers race-specific resistance to the pathogen, Cochliobolus carbonum. We have used transposon mutagenesis to tag, clone, and characterize several H^_M^_1^_ alleles. The gene can be used as a selectable marker in conjunction with the toxin produced by C. carbonum.