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公开(公告)号：EP2054501A2
公开(公告)日：2009-05-06
申请号：EP07870168.7
申请日：2007-10-08
申请人： PROMEGA CORPORATION
IPC分类号： C12N1/00
CPC分类号： C12N5/0612 , C12N1/06 , C12N5/061 , C12N15/1013
摘要： Disclosed are methods for differential extraction of a target component from a sample which is predominantly composed of other types of non-target cells that can be lysed using methods that do not lyse the target cells, so that the target material can be purified away from the lysed non-target material. One exemplary method is directed to isolating sperm cells from an aqueous sample and kits for performing same.
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公开(公告)号：EP1155149A1
公开(公告)日：2001-11-21
申请号：EP00908725.5
申请日：2000-02-18
申请人： PROMEGA CORPORATION
发明人： SHULTZ, John, William , LEWIS, Martin, K. , LEIPPE, Donna , MANDREKAR, Michelle , KEPHART, Daniel , RHODES, Richard, B. , ANDREWS, Christine, Ann , HARTNETT, James, Robert , GU, Trent , OLSON, Ryan, J. , WOOD, Keith, V. , WELCH, Roy
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/04 , C12Q1/66 , C12Q1/6827 , C12Q2535/131 , C12Q2521/319 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101 , C12Q2565/627
摘要： Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined endogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.
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3.发明公开METHODS FOR DETERMINING THE PRESENCE OF NUCLEIC ACID TARGET SEQUENCES AND APPLICATIONS THEREOF 审中-公开用于确定NUCLEIC靶序列及其用途
公开(公告)号：EP1155151A1
公开(公告)日：2001-11-21
申请号：EP00911863.9
申请日：2000-02-18
申请人： PROMEGA CORPORATION
发明人： SHULTZ, John, W. , LEWIS, Martin, K. , LEIPPE, Donna , MANDREKAR, Michelle , KEPHART, Daniel , RHODES, Richard, B. , ANDREWS, Christine, Ann , HARTNETT, James, R. , GU, Trent , OLSON, Ryan, J. , WOOD, Keith, V. , WELCH, Roy
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/04 , C12Q1/66 , C12Q1/6823 , C12Q1/6827 , Y10T436/24 , C12Q2565/627 , C12Q2535/131 , C12Q2521/319 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101
摘要： Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined exogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, determination of viral load, genotyping, medical marker diagnostics, and the like.
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