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公开(公告)号：EP1685247A2
公开(公告)日：2006-08-02
申请号：EP04821603.0
申请日：2004-09-29
申请人： PROMEGA CORPORATION
摘要： The invention provides vectors and methods for directional cloning.
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公开(公告)号：EP1259529A2
公开(公告)日：2002-11-27
申请号：EP01913164.8
申请日：2001-02-28
申请人： PROMEGA CORPORATION
发明人： GU, Trent , HUANG, Fen , HARTNETT, James, Robert
IPC分类号： C07H21/04 , C12N9/12 , C12N15/00 , C12N15/09 , C12N15/63 , C12N15/70 , C12N15/74 , C12N5/00 , C12N5/02 , C12N1/20 , C12Q1/68
CPC分类号： C12N9/1252 , C12N9/1276
摘要： The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species Thermoactinomyces vulgaris. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. vulgaris DNA polymerase in sequencing, reverse transcription, and amplification reactions.
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公开(公告)号：EP1155149A1
公开(公告)日：2001-11-21
申请号：EP00908725.5
申请日：2000-02-18
申请人： PROMEGA CORPORATION
发明人： SHULTZ, John, William , LEWIS, Martin, K. , LEIPPE, Donna , MANDREKAR, Michelle , KEPHART, Daniel , RHODES, Richard, B. , ANDREWS, Christine, Ann , HARTNETT, James, Robert , GU, Trent , OLSON, Ryan, J. , WOOD, Keith, V. , WELCH, Roy
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/04 , C12Q1/66 , C12Q1/6827 , C12Q2535/131 , C12Q2521/319 , C12Q2565/301 , C12Q2565/1015 , C12Q2537/101 , C12Q2565/627
摘要： Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined endogenous nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.
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5.发明公开
公开(公告)号：EP1266033A1
公开(公告)日：2002-12-18
申请号：EP01913200.0
申请日：2001-02-28
申请人： PROMEGA CORPORATION
发明人： GU, Trent , HUANG, Fen , HARTNETT, James, Robert
CPC分类号： C12N15/1096 , C12N9/1276 , C12P19/34 , C12Q1/6844 , C12Q1/686 , C12Q1/689 , C12Q2527/101 , C12Q2521/107
摘要： The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.
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