公开(公告)号：EP3936857A1

公开(公告)日：2022-01-12

申请号：EP21190541.9

申请日：2007-08-31

Applicant: Pacific Biosciences Of California, Inc.

Inventor： LUNDQUIST, Paul ,  TURNER, Stephen

IPC: G01N21/64 ,  G01N21/77 ,  G02B6/43

Abstract: Substrates, systems and methods for analyzing materials that include waveguide arrays disposed upon or within the substrate such that evanescent fields emanating from the waveguides illuminate materials disposed upon or proximal to the surface of the substrate, permitting analysis of such materials. The substrates, systems and methods are used in a variety of analytical operations, including, inter alia, nucleic acid analysis, including hybridization and sequencing analyses, cellular analyses and other molecular analyses.

公开(公告)号：EP3629011A3

公开(公告)日：2020-05-06

申请号：EP19209994.3

申请日：2009-09-15

Applicant: Pacific Biosciences Of California, Inc.

Inventor： ZHONG, Cheng, Frank ,  LUNDQUIST, Paul ,  FOQUET, Mathieu ,  KORLACH, Jonas ,  BAYANDORIAN, Hovig

IPC: G01N21/64 ,  G01N21/77

Abstract: The present invention provides an integrated optical device (2300), comprising:
a) a first component (2302) comprising a waveguide (2312) disposed upon or within a substrate and a plurality of nanometer-scale apertures (2308) comprising analyte regions (2310), wherein the analyte regions (2310) are disposed sufficiently proximal to a core of the waveguide (2312) to be illuminated by an evanescent field emanating from the core when optical energy is passed through the waveguide;
b) a second component (2304) comprising a microlens array that collects optical energy signals from the analyte regions (2310) and directs the optical energy signals so collected to a detector (2320); and
c) a third component (2306) comprising the detector (2320).

公开(公告)号：EP2959283A1

公开(公告)日：2015-12-30

申请号：EP14754479.5

申请日：2014-02-21

Applicant: Pacific Biosciences of California, Inc.

Inventor： LUNDQUIST, Paul ,  TURNER, Stephen

IPC: G01N21/17 ,  G01N21/63 ,  G01N21/64

CPC classification number: C12Q1/6874 ,  G01N21/6428 ,  G01N21/6454 ,  G01N21/648 ,  G01N21/7746 ,  G01N2021/7789 ,  G01N2201/08 ,  G02B6/0229 ,  G02B2006/12147

Abstract: Optical analytical devices and their methods of use are provided. The devices are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices include integrated illumination elements and optical waveguides for illumination of the optical reactions. The devices further provide for the efficient coupling of optical excitation energy from the waveguides to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination using features such as spectra, amplitude, and time resolution, or combinations thereof. The devices of the invention are well suited for miniaturization and high throughput.

公开(公告)号：EP2936222A1

公开(公告)日：2015-10-28

申请号：EP13863794.7

申请日：2013-12-16

Applicant: Pacific Biosciences Of California, Inc.

Inventor： GROT, Annette ,  WANG, Shang ,  CALLEBAUT, Hans ,  LUNDQUIST, Paul ,  TURNER, Stephen

IPC: G02B6/10

CPC classification number: G02B6/0016 ,  B82Y15/00 ,  B82Y20/00 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/6486 ,  G01N21/7703 ,  G02B6/024 ,  G02B6/10 ,  G02B6/12004 ,  G02B6/122 ,  G02B23/2461

Abstract: Optical analytical devices and their methods of use are provided. The devices are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices include optical waveguides for illumination of the optical reactions. The devices further provide for the efficient coupling of optical excitation energy from the waveguides to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination using features such as spectra, amplitude, and time resolution, or combinations thereof. The devices of the invention are well suited for miniaturization and high throughput.

公开(公告)号：EP1984670B1

公开(公告)日：2014-07-30

申请号：EP07750409.0

申请日：2007-02-09

Applicant: Pacific Biosciences of California, Inc.

Inventor： LUNDQUIST, Paul ,  ZACCARIN, Denis ,  LACROIX, Yves ,  MAXHAM, Mark ,  FOQUET, Mathieu ,  TURNER, Stephen

IPC: G01N21/64 ,  G01J3/14 ,  G01J3/28 ,  G01J3/10 ,  G01J3/02

CPC classification number: G01N21/6452 ,  G01J3/02 ,  G01J3/0224 ,  G01J3/10 ,  G01J3/14 ,  G01J3/2803 ,  G01N21/6445 ,  G01N21/6456 ,  G01N21/648 ,  G01N2021/6417 ,  G01N2021/6441

公开(公告)号：EP4425153A2

公开(公告)日：2024-09-04

申请号：EP24176091.7

申请日：2016-06-11

Applicant: Pacific Biosciences Of California, Inc.

Inventor： WANG, Shang ,  FOQUET, Mathieu ,  LUNDQUIST, Paul ,  RULISON, Aaron ,  MCDONALD, Mark ,  HERRMANN, Ariel

IPC: G01N21/64

CPC classification number: G02B6/34 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/7703 ,  G02B6/124 ,  G02B6/4221 ,  G02B2006/1213520130101

Abstract: Integrated target waveguide devices and optical analytical systems comprising such devices are provided. The target devices include an optical coupler that is optically coupled to an integrated waveguide and that is configured to receive optical input from an optical source through free space, particularly through a low numerical aperture interface. The devices and systems are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices provide for the efficient and reliable coupling of optical excitation energy from an optical source to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and dis - crimination. The devices and systems are well suited for miniaturization and high throughput.

公开(公告)号：EP4123294A1

公开(公告)日：2023-01-25

申请号：EP22194814.4

申请日：2013-12-16

Applicant: Pacific Biosciences Of California, Inc.

Inventor： GROT, Annette ,  WANG, Shang ,  CALLEBAUT, Hans ,  LUNDQUIST, Paul ,  TURNER, Stephen

IPC: G01N21/64 ,  G01N21/77 ,  G02B6/10 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y20/00 ,  G01N33/543 ,  G01N33/553 ,  G01N21/25 ,  C12Q1/6874 ,  G02B6/12

Abstract: An analytical device comprising:
an optical waveguide comprising an optical core (748) and a cladding (750);
a metallic layer (744) disposed on a surface of the cladding; and
a plurality of nanometer-scale apertures (742) disposed in the metallic layer such that optical energy passing through the optical core of the optical waveguide illuminates the plurality of apertures with an evanescent field emanating from the waveguide;
wherein the cladding has a thickness that is the distance between the metal layer and the optical core, wherein the thickness of the cladding is decreased in regions adjacent to the nanoscale apertures and wherein the thickness of the cladding is increased in regions remote from the nanoscale apertures.

公开(公告)号：EP3308204A1

公开(公告)日：2018-04-18

申请号：EP16808488.7

申请日：2016-06-11

Applicant: Pacific Biosciences of California, Inc.

Inventor： WANG, Shang ,  FOQUET, Mathieu ,  LUNDQUIST, Paul ,  RULISON, Aaron ,  MCDONALD, Mark ,  HERRMANN, Ariel

IPC: G02B6/26 ,  G02B6/34

CPC classification number: G02B6/124 ,  G01N21/03 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/6486 ,  G01N21/7703 ,  G01N21/774 ,  G01N2021/0346 ,  G02B6/34 ,  G02B6/4221 ,  G02B2006/12135

Abstract: Integrated target waveguide devices and optical analytical systems comprising such devices are provided. The target devices include an optical coupler that is optically coupled to an integrated waveguide and that is configured to receive optical input from an optical source through free space, particularly through a low numerical aperture interface. The devices and systems are useful in the analysis of highly multiplexed optical reactions in large numbers at high densities, including biochemical reactions, such as nucleic acid sequencing reactions. The devices provide for the efficient and reliable coupling of optical excitation energy from an optical source to the optical reactions. Optical signals emitted from the reactions can thus be measured with high sensitivity and discrimination. The devices and systems are well suited for miniaturization and high throughput.

公开(公告)号：EP2331934A2

公开(公告)日：2011-06-15

申请号：EP09814888.5

申请日：2009-09-15

Applicant: Pacific Biosciences of California, Inc.

Inventor： ZHONG, Cheng, Frank ,  LUNDQUIST, Paul ,  FOQUET, Mathieu ,  KORLACH, Jonas ,  BAYANDORIAN, Hovig

IPC: G01N21/00 ,  G01N33/483 ,  G01B9/02

Abstract: This invention provides substrates for use in various applications, including single-molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided.

公开(公告)号：EP1920277A2

公开(公告)日：2008-05-14

申请号：EP06813384.2

申请日：2006-08-10

Applicant: Pacific Biosciences of California, Inc.

Inventor： LUNDQUIST, Paul ,  TURNER, Stephen ,  ZACCARIN, Denis

IPC: G02B6/00

CPC classification number: G01N21/6452 ,  G01J3/2803 ,  G01J3/36 ,  G01N21/6428

Abstract: Methods and systems for monitoring a plurality of different optical signals from a single source of such signals, where each such different optical signal is spatially separated from other such signals and directed to different detectors or locations upon a single detector, which direction is generally accomplished through the use of a small number of optical components and/or manipulations.