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公开(公告)号：EP2553102A2
公开(公告)日：2013-02-06
申请号：EP11766437.5
申请日：2011-03-28
申请人： Pfenex Inc.
CPC分类号： C12N9/1051 , C07K14/21 , C07K14/235 , C07K14/28 , C07K14/34 , C07K2319/036 , C12N9/1077 , C12N15/78 , C12P21/00 , C12P21/02 , C12Y204/01 , C12Y204/02036 , C12Y304/24068 , G01N33/573
摘要： The present invention relates to the field of recombinant toxin protein production in bacterial hosts. In particular, the present invention relates to production processes for obtaining high levels of a recombinant CRM 197, Diphtheria Toxin, Pertussis Toxin, Tetanus Toxoid Fragment C, Cholera Toxin B, Cholera holotoxin, and Pseudomonas Exotoxin A, from a bacterial host.
摘要翻译： 本发明涉及在细菌宿主中产生重组毒素蛋白的领域。 具体而言，本发明涉及从细菌宿主获得高水平重组CRM 197，白喉毒素，百日咳毒素，破伤风类毒素片段C，霍乱毒素B，霍乱全毒素和假单胞菌外毒素A的生产方法。
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公开(公告)号：EP1709170B1
公开(公告)日：2018-02-21
申请号：EP05705852.1
申请日：2005-01-18
申请人： Pfenex Inc.
发明人： RETALLACK, Diane, M. , SQUIRES, Charles, H. , WATKINS, David, C. , GAERTNER, Frank, H. , LEE, Stacey, Lynn , SHUTTER, Robert
摘要： The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E.coli in comparable circumstances. Processes for improved production of isolated mammalian, particularly human, proteins are provided.
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3.发明授权PRODUCTION OF RECOMBINANT PROTEINS UTILIZING NON-ANTIBIOTIC SELECTION METHODS AND THE INCORPORATION OF NON-NATURAL AMINO ACIDS THEREIN 有权利用非抗生素选择方法生产重组蛋白及其中掺入非天然氨基酸的重组蛋白
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公开(公告)号：EP2553102B1
公开(公告)日：2015-12-09
申请号：EP11766437.5
申请日：2011-03-28
申请人： Pfenex Inc.
CPC分类号： C12N9/1051 , C07K14/21 , C07K14/235 , C07K14/28 , C07K14/34 , C07K2319/036 , C12N9/1077 , C12N15/78 , C12P21/00 , C12P21/02 , C12Y204/01 , C12Y204/02036 , C12Y304/24068 , G01N33/573
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公开(公告)号：EP1687324B1
公开(公告)日：2012-08-22
申请号：EP04817876.8
申请日：2004-11-22
申请人： Pfenex, Inc.
IPC分类号： C07H21/04 , C07H7/00 , C07H19/00 , C12N1/21 , C12N15/10 , C12N15/63 , C12N15/64 , C12N15/78 , C12N15/72
CPC分类号： C07K14/21 , C07K14/61 , C07K16/00 , C07K16/16 , C07K2317/622 , C07K2319/036 , C12N15/625 , C12N15/78
摘要： Improved methods and prokaryotic expression systems for producing recombinant proteins using Sec-system secretion signal peptides are described using Pseudomonas fluorescens Sec secretion systems. Specific novel Sec-system secretion signal peptides are described, as are fusion proteins and coding sequences for improved secretion of recombinant proteins and peptides.
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6.发明公开
公开(公告)号：EP2430164A2
公开(公告)日：2012-03-21
申请号：EP10775323.8
申请日：2010-05-10
申请人： Pfenex, Inc.
摘要： Provided herein are methods and compositions for expression of a nucleic acid construct comprising nucleic acids encoding a) a recombinant polypeptide, and b) a prototrophy-restoring enzyme in a host cell that is auxotrophic for at least one metabolite. In various embodiments, the host cell is auxotrophic for a nitrogenous base compound or an amino acid. The invention involves introducing an analogue into the growth media for the host cell such that the analogue is incorporated into the recombinant polypeptide or a nucleic acid coding sequence thereof. In various embodiments, the compositions and methods disclosed herein result in improved recombinant protein expression compared to expression of recombinant protein in an antibiotic selection system, or compared to expression of the recombinant protein in an expression system that lacks a metabolite analogue.
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