公开(公告)号：EP1959018A1

公开(公告)日：2008-08-20

申请号：EP08075051.6

申请日：2003-03-18

申请人： Plant Research International B.V.

发明人： Bakker, Hendrikus Antonius Cornelius ,  Florack, Dionisius Elisabeth Antonius ,  Bosch, Hendrik Jan ,  Rouwendal, Gerard Johan Adolph

IPC分类号： C12N15/82 ,  C12N9/10

CPC分类号： C12N9/1051 ,  C07K2317/13 ,  C07K2317/41 ,  C07K2319/02 ,  C12N15/8257 ,  C12N15/8258

摘要： The invention is directed to methods for optimizing glycan processing in organisms (and in particular, plants) so that a glycoprotein having complex type bi-antennary glycans and thus 5 containing galactose residues on both arms and which are devoid of (or reduce in) xylose and fucose can be obtained. The invention is further directed to said glycoprotein obtained and host system comprising said protein.

摘要翻译： 本发明涉及用于优化生物（特别是植物）中的聚糖加工的方法，使得具有复合型双触角聚糖的糖蛋白，因此在两个臂上含有半乳糖残基并且没有（或减少）木糖的糖蛋白 并且可以获得岩藻糖。 本发明还涉及获得的所述糖蛋白和包含所述蛋白质的宿主系统。

公开(公告)号：EP1264891A1

公开(公告)日：2002-12-11

申请号：EP01202078.0

申请日：2001-05-31

申请人： Plant Research International B.V.

发明人： Rouwendal, Gerard Johan Adolph

IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N5/10

CPC分类号： C12N15/8213 ,  C12N15/8209

摘要： The invention relates to the field of transforming plant cells and the modification of plant genomes. The invention provides a method for transforming a plant cell comprising providing said cell with site-specific recombinase activity.

摘要翻译： 本发明涉及植物细胞转化和植物基因组修饰的领域。 本发明提供了一种用于转化植物细胞的方法，包括提供所述细胞具有位点特异性重组酶活性。

公开(公告)号：EP1867722A1

公开(公告)日：2007-12-19

申请号：EP06076248.1

申请日：2006-06-16

申请人： Plant Research International B.V.

发明人： Rouwendal, Gerard Johan Adolph ,  Bosch, Hendrik Jan ,  Florack, Dionisus Elisabeth Antonius

IPC分类号： C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8258 ,  C12N15/8245 ,  C12N15/8257

摘要： The invention comprises a method to produce a Lewis X epitope on N-linked glycans on glycoproteins in a plant, plant part or plant cells, comprising expression of a nucleotide sequence encoding a galactosyltransferase, which catalyzes the addition of galactose in β1,4-linkage to N-linked glycans, and expression of a nucleotide sequence encoding a fucosyltransferase, which catalyzes the addition of fucose in α1,3-linkage to the N-acetylglucosamine of Galβ1-4GlcNAc structures on N-linked glycans, and expression of one or more nucleotide sequences encoding one or more glycoproteins, which are able to be provided with an N-linked glycan, in said plant, plant part or plant cell. The fucosyltransferase comprises preferably the catalyticaal active domain of FUT9 from T. nigroviridis. Also part of the invention are hybrid enzymes with said domain and an N-terminal part which confers expression in the Golgi apparatus.

摘要翻译： 本发明包括在植物，植物部分或植物细胞中产生N-连接的聚糖上的糖蛋白上的路易斯X表位的方法，其包括编码半乳糖基转移酶的核苷酸序列的表达，所述核苷酸序列催化加入二 至N-连接的聚糖，以及编码岩藻糖基转移酶的核苷酸序列的表达，其催化向N-连接的聚糖中的Gal21-4GlcNAc结构的N-乙酰葡糖胺以±1,3键的形式加入岩藻糖，以及表达一个或多个 在所述植物，植物部分或植物细胞中编码能够提供有N-连接的聚糖的一种或多种糖蛋白的更多核苷酸序列。 岩藻糖基转移酶优选包含来自黑曲霉病毒的FUT9的催化活性结构域。 本发明的一部分是具有所述结构域的杂合酶和赋予高尔基体表达的N末端部分。