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    NANOPORE-BASED NUCLEIC ACID ANALYSIS WITH MIXED FRET DETECTION
    2.
    发明公开
    NANOPORE-BASED NUCLEIC ACID ANALYSIS WITH MIXED FRET DETECTION 有权
    具有混合FRET检测NANO基于多孔NUCLEIC分析

    公开(公告)号：EP3004385A2

    公开(公告)日：2016-04-13

    申请号：EP14801275.0

    申请日：2014-05-23

    申请人： Quantapore Inc.

    发明人： HUBER, Martin ,  CLANCY, Bason E. ,  HARDENBOL, Paul

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2565/101 ,  C12Q2565/631

    摘要： Various methods, systems and devices for optical detection and analysis of polymers, such as polynucleotides, using nanopores, e.g., for determining sequences of nucleic acids, are provided herein. In certain variations, methods and systems for determining a nucleotide sequence of a polynucleotide, which include measuring mixed FRET signals as a polynucleotide translocates through a nanopore and determining a nucleotide sequence of the polynucleotide from the mixed FRET signals, are provided.

    EFFICIENT OPTICAL ANALYSIS OF POLYMERS USING ARRAYS OF NANOSTRUCTURES
    5.
    发明公开
    EFFICIENT OPTICAL ANALYSIS OF POLYMERS USING ARRAYS OF NANOSTRUCTURES 审中-公开
    聚合物纳米结构阵列的有效光学分析

    公开(公告)号：EP3209800A1

    公开(公告)日：2017-08-30

    申请号：EP15852311.8

    申请日：2015-10-23

    申请人： Quantapore Inc.

    发明人： HUBER, Martin ,  DAVIDSON, Stuart

    IPC分类号： C12Q1/68 ,  G01N27/447 ,  G01N27/453

    CPC分类号： C12Q1/6869 ,  C12Q1/6874 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/76 ,  G01N33/48721 ,  G01N2021/6439 ,  G01N2201/06113 ,  C12Q2535/122 ,  C12Q2563/107 ,  C12Q2565/125 ,  C12Q2565/631

    摘要： The invention is directed to methods and apparatus for detecting sequences of optical signals from parallel reactions on an array of nanostructures, such as nanopores, nanowells, or nanoparticles. In accordance with the invention, an array of nanostructures is provided, each nanostructure comprising a reaction site and each capable of confining a reaction that generates a sequence of optical signals, and the nanostructures of the array being arranged in clusters each comprising a number of nanostructures. Each different cluster is disposed within a different resolution limited area and the number of nanostructures in each cluster is either greater than one or a random variable with an average value greater than zero. Optical signals from reactions in the nanostructures are detected by an optical system operatively associated with the array.

    摘要翻译： 本发明涉及用于检测来自纳米结构阵列（例如纳米孔，纳米孔或纳米颗粒）上的平行反应的光学信号序列的方法和装置。 根据本发明，提供了纳米结构阵列，每个纳米结构包含反应位点并且每个纳米结构能够限制产生光信号序列的反应，并且阵列的纳米结构排列成簇，每个簇包含许多纳米结构 。 每个不同的聚类都位于不同的分辨率有限区域内，每个聚类中纳米结构的数量要么大于1个，要么随机变量的平均值大于零。 来自纳米结构中的反应的光学信号由与阵列可操作地相关联的光学系统检测。

    NANOPORE-BASED POLYMER ANALYSIS WITH MUTUALLY-QUENCHING FLUORESCENT LABELS
    7.
    发明公开
    NANOPORE-BASED POLYMER ANALYSIS WITH MUTUALLY-QUENCHING FLUORESCENT LABELS 审中-公开
    基于纳米孔的荧光标记荧光标记聚合物分析

    公开(公告)号：EP3204519A1

    公开(公告)日：2017-08-16

    申请号：EP15849196.9

    申请日：2015-10-08

    申请人： Quantapore Inc.

    发明人： HUBER, Martin

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q2563/107 ,  C12Q2565/101 ,  C12Q2565/631

    摘要： The invention is directed to a method for determining a monomer sequence of a polymer that is translocated through a nanopore. Monomers of the polymer are labeled with fluorescent labels such that in free solution fluorescent labels of adjacent monomers substantially quench each other and wherein the nanopore constrains fluorescent labels within its bore into a constrained state wherein no detectable fluorescent signal can be generated. By exciting the fluorescent label of each monomer as it exits the nanopore and transitions from a constrained state to a quenched state with an adjacent fluorescent label, a fluorescent signal can be generated by the exiting fluorescent label that allows its monomer to be identified, thereby permitting a monomer sequence to be determined from a sequence of fluorescent signals as the polymer translocates through the nanopore.

    摘要翻译： 本发明涉及确定通过纳米孔移位的聚合物的单体序列的方法。 用荧光标记标记聚合物的单体，使得在自由溶液中相邻单体的荧光标记基本上彼此猝灭，并且其中纳米孔将其孔内的荧光标记限制在约束状态，其中不能产生可检测的荧光信号。 通过激发每个单体的荧光标签离开纳米孔并从相邻荧光标签从受约束状态过渡到淬灭状态，荧光信号可以通过退出荧光标签产生，从而允许其单体被识别，由此允许 当聚合物移位通过纳米孔时，由荧光信号序列确定单体序列。