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公开(公告)号：EP0216080B1
公开(公告)日：1993-03-03
申请号：EP86110534.4
申请日：1986-07-30
申请人： RIKAGAKU KENKYUSHO
CPC分类号： C12N9/2437 , C07K14/245 , C12N9/248 , C12N15/67 , C12N15/70 , C12N15/75
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公开(公告)号：EP0244873A3
公开(公告)日：1988-12-21
申请号：EP87106710
申请日：1987-05-08
摘要： A cell culture method proliferates microorganisms, particularly fungi such as molds, actinomycetes, animal cells, plant cells and so on to produce useful substances such as antibiotics, enzymes, proteins, polysaccharides, physiological active substances, and animal and plant hormones. Porous material including the medium and cells to be proliferated is moved, whereby air is supplied to the surface of the porous material.
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公开(公告)号：EP0244873B1
公开(公告)日：1992-08-05
申请号：EP87106710.4
申请日：1987-05-08
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公开(公告)号：EP0269086A3
公开(公告)日：1989-09-20
申请号：EP87117395.1
申请日：1987-11-25
CPC分类号： C12M25/20
摘要： A cell culture apparatus is described which comprises a fluidized bed culture tank body in which cell attached porous material is fluidized by air flow, and a pre-culture tank for supplying medium to said fluidized bed culture tank body corresponding to the amount of the liquid medium which is taken out of said fluidized bed culture tank body. The cell culture apparatus can be used for the continuous production of a metabolite.
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公开(公告)号：EP0244873A2
公开(公告)日：1987-11-11
申请号：EP87106710.4
申请日：1987-05-08
摘要： A cell culture method proliferates microorganisms, particularly fungi such as molds, actinomycetes, animal cells, plant cells and so on to produce useful substances such as antibiotics, enzymes, proteins, polysaccharides, physiological active substances, and animal and plant hormones. Porous material including the medium and cells to be proliferated is moved, whereby air is supplied to the surface of the porous material.
摘要翻译： 细胞培养方法增殖微生物，特别是真菌如霉菌，放线菌，动物细胞，植物细胞等，以产生有用的物质，如抗生素，酶，蛋白质，多糖，生理活性物质和动植物激素。 包括培养基和增殖细胞的多孔材料被移动，由此向多孔材料的表面供应空气。
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公开(公告)号：EP0269086A2
公开(公告)日：1988-06-01
申请号：EP87117395.1
申请日：1987-11-25
CPC分类号： C12M25/20
摘要： A cell culture apparatus is described which comprises a fluidized bed culture tank body in which cell attached porous material is fluidized by air flow, and a pre-culture tank for supplying medium to said fluidized bed culture tank body corresponding to the amount of the liquid medium which is taken out of said fluidized bed culture tank body. The cell culture apparatus can be used for the continuous production of a metabolite.
摘要翻译： 描述了一种细胞培养装置，其包括其中细胞附着的多孔材料通过气流流化的流化床培养箱体，以及用于向所述流化床培养罐体供给培养基的预培养罐，其对应于液体培养基的量 其从所述流化床培养罐体中取出。 细胞培养装置可用于连续生产代谢物。
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公开(公告)号：EP0216080A3
公开(公告)日：1988-04-20
申请号：EP86110534
申请日：1986-07-30
申请人： RIKAGAKU KENKYUSHO
CPC分类号： C12N9/2437 , C07K14/245 , C12N9/248 , C12N15/67 , C12N15/70 , C12N15/75
摘要： Desribed is plasmid pEAP7AP containing a DNA region which is capable of inducing the extracellular secretion of a useful, physiologically active substance in a transformed host and a promoter DNA region regulating the expression of the former DNA region. Described is also a microorganism transformed with the plasmid and a process for the production of useful substances by culturing the microorganism.
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公开(公告)号：EP0216080A2
公开(公告)日：1987-04-01
申请号：EP86110534.4
申请日：1986-07-30
申请人： RIKAGAKU KENKYUSHO
CPC分类号： C12N9/2437 , C07K14/245 , C12N9/248 , C12N15/67 , C12N15/70 , C12N15/75
摘要： Desribed is plasmid pEAP7AP containing a DNA region which is capable of inducing the extracellular secretion of a useful, physiologically active substance in a transformed host and a promoter DNA region regulating the expression of the former DNA region. Described is also a microorganism transformed with the plasmid and a process for the production of useful substances by culturing the microorganism.
摘要翻译： 描述了含有能够在转化的宿主中诱导有用的生理活性物质的细胞外分泌的DNA区的质粒pEAP7 DELTA P和调节前DNA区的表达的启动子DNA区。 还描述了用质粒转化的微生物和通过培养微生物生产有用物质的方法。
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