公开(公告)号：EP2558478A4

公开(公告)日：2013-10-23

申请号：EP11769451

申请日：2011-04-12

申请人： SOMALOGIC INC

发明人： ROHLOFF JOHN ,  JANJIC NEBOJSA ,  CARTER JEFFREY D ,  FOWLER CATHERINE

IPC分类号： C07H19/073 ,  C07H19/10 ,  C07H21/04

CPC分类号： C07H19/10 ,  C07H19/06 ,  C07H21/04 ,  C12N15/115 ,  C12N2310/335

摘要： The present disclosure relates to the field of nucleic acid chemistry, specifically to 5-position modified uridines as well as phosphoramidite and triphosphate derivatives thereof. The present disclosure also relates to methods of making and using the same.

摘要翻译： 本公开涉及核酸化学领域，具体涉及5位修饰的尿苷以及其亚磷酰胺和三磷酸衍生物。 本公开还涉及制备和使用该方法。

公开(公告)号：EP3071588A4

公开(公告)日：2017-08-02

申请号：EP14864118

申请日：2014-11-19

申请人： SOMALOGIC INC

发明人： ROHLOFF JOHN ,  JANJIC NEBOJSA ,  GAWANDE BHARATNATHU

IPC分类号： C07H19/06 ,  C07H1/00 ,  C07H21/00 ,  C12Q1/68

CPC分类号： C07H19/06 ,  C07H1/00 ,  C07H21/00 ,  C12Q1/6811 ,  C12Q2525/117

摘要： Described herein are 5-position modified cytosines of formula I, nucleotides and nucleosides as well as phosphoramidites and triphosphates derivatives thereof. Further provided are methods of making and using the same, and compositions and uses of the modified nucleosides as part of a nucleic acid molecule (e.g., aptamer). Those modified nucleotides, bearing an N-substituted-carboxamine group at the 5 -position, are valuable tools for improving in vitro selection of protein-binding aptamers (SELEX process). wherein R is independently a -(CH 2 ) n , wherein n is 0,1,2,3,4,5,6,7,8,9 or 10; R X1 is independently selected from the group consisting of

摘要翻译： 本文描述了5位修饰的胞嘧啶核苷酸和核苷以及其亚磷酰胺和三磷酸酯衍生物。 进一步提供了制备和使用它们的方法，以及修饰的核苷作为核酸分子（例如适体）的一部分的组合物和用途。