公开(公告)号：EP3027775A4

公开(公告)日：2017-04-12

申请号：EP14832389

申请日：2014-08-01

Applicant: STC UNM

Inventor： EDWARDS JEREMY SCOTT

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6841 ,  C12Q2523/303 ,  C12Q2543/10 ,  C12Q2523/307

Abstract: This disclosure describes, in one aspect, methods for DNA sequencing and performing epigenomic analyses. Generally, the methods include immobilizing a plurality of copies of a DNA molecule on a surface, stretching at least a portion of the immobilized DNA molecules, and sequencing at least a portion of the immobilized, stretched DNA molecules.

Abstract translation: 在一个方面，本公开描述了用于DNA测序和进行表观基因组分析的方法。 通常，这些方法包括将多个DNA分子拷贝固定在表面上，拉伸至少一部分固定的DNA分子，并对至少一部分固定的，拉伸的DNA分子进行测序。

公开(公告)号：EP3074134A4

公开(公告)日：2017-07-12

申请号：EP14865949

申请日：2014-11-26

Applicant: STC UNM

Inventor： BRUECK STEVEN R J ,  EDWARDS JEREMY SCOTT ,  NEUMANN ALEXANDER ,  KUZNETSOVA YULIYA ,  MENDOZA EDGAR A

IPC: B01L7/00 ,  C12M1/34 ,  C12Q1/68

CPC classification number: G01N33/48721 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0896 ,  B01L2300/16 ,  B01L2300/168 ,  B01L2400/0421 ,  C12Q1/6869 ,  C23C16/345 ,  C23C16/45525 ,  C23C16/50 ,  G01N21/65 ,  G01N2021/653 ,  G01N2201/061 ,  C12Q2563/155 ,  C12Q2565/631

Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

Abstract translation: 用于长读取，无标记，光学纳米孔长链分子测序的方法和设备。 通常，本公开内容描述了基于纳米通道整合以递送具有宽间隔（>波长），〜1nm孔径“曲折”纳米孔的单个长链分子的新型测序技术，其缓慢移位足以提供大规模平行， 使用光学技术的单基准分辨率。 使用简单的胶体纳米粒子的新型定向自组装纳米制造方案用于在展开长链分子的纳米通道上形成纳米孔阵列。 在纳米颗粒阵列的表面，工程等离子体激元/极化激元结构中的强局部电磁场允许使用光学技术实现单基极分辨率。

公开(公告)号：EP3055431A4

公开(公告)日：2017-05-10

申请号：EP14852570

申请日：2014-09-24

Applicant: STC UNM

Inventor： EDWARDS JEREMY SCOTT

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6853 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2525/161 ,  C12Q2525/179 ,  C12Q2563/179

Abstract: The disclosure describes a method for sequencing long portions of DNA sequence by assembling a plurality of shorter polynucleotide reads. Generally, The method includes annealing a plurality of primers to a denatured DNA molecule, appending a barcode polynucleotide to the 5′ end of the primer, subjecting the DNA molecules to a plurality of cycles of (1) pooling, (2) dividing, and (3) appending a barcode polynucleotide to the 5′ end of the primer, sequencing the barcode polynucleotides and the genomic DNA, and assembling the short read polynucleotide sequences having identical barcode polynucleotides.

Abstract translation: 本公开描述了通过组装多个较短的多核苷酸读数对DNA序列的长部分进行测序的方法。 通常，该方法包括将多个引物退火至变性的DNA分子，将条形码多核苷酸附加至引物的5'末端，使DNA分子经历多次（1）合并，（2）分裂和 （3）将条形码多核苷酸附加到引物的5'末端，对条形码多核苷酸和基因组DNA进行测序，并组装具有相同条形码多核苷酸的短读取多核苷酸序列。