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    Method and Apparatus for Isolating and Purifying Nucleic Acid by Single Bead
    4.
    发明公开
    Method and Apparatus for Isolating and Purifying Nucleic Acid by Single Bead 有权
    方法和装置用于核酸通过单个珠子的分离和纯化

    公开(公告)号：EP1903338A2

    公开(公告)日：2008-03-26

    申请号：EP07105732.7

    申请日：2007-04-05

    申请人： Samsung Electronics Co., Ltd.

    发明人： Yoo, Chang-eun ,  Jeong, Sung-young ,  Kim, Young-rok

    IPC分类号： G01N33/543 ,  C12N15/10 ,  B01J20/00

    CPC分类号： B01J20/28009 ,  B01D15/1807 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/327 ,  C08F20/02 ,  C08F26/06 ,  C12N1/066 ,  C12N13/00

    摘要： Provided is a method of isolating and purifying nucleic acid from cells or viruses, the method comprising: mixing a sample containing cells or viruses with a solution comprising beads dispersed in a binding buffer to bind the cells or viruses with the beads; separating the beads having the cells or viruses bound thereto and then washing the beads with the buffer; disrupting the cells or viruses bound to the beads to bind nucleic acid released from the cells or viruses with the beads; washing the beads having nucleic acid bound thereto with the buffer; and adding a nucleic acid eluting buffer to the beads to elute nucleic acid bound to the beads, wherein a compound represented by Formula 1 is bound to the surface of the bead.

    摘要翻译： 本发明提供一种分离和纯化从细胞或病毒，该方法包括核酸的方法，包括：将含有与溶液包含分散在结合细胞或病毒与珠的结合缓冲液珠细胞或病毒的样品; 分离具有的细胞或病毒结合到其上，然后洗涤缓冲液中的珠的珠; 破碎细胞或结合在珠，以核酸从细胞或病毒与所述珠粒中释放结合病毒; 洗涤具有核酸结合用缓冲液在其上的小珠; 和添加核酸洗脱缓冲液珠洗脱核酸结合到珠子上，worin由式1表示的化合物结合至珠子的表面上。

    Hybridization chamber agitation device using pump and valves
    7.
    发明公开
    Hybridization chamber agitation device using pump and valves 有权
    Rührvorrichtungund Verfahren einer Hybridisierungskammer umfassend Pump und Ventile

    公开(公告)号：EP1666134A2

    公开(公告)日：2006-06-07

    申请号：EP05025460.6

    申请日：2005-11-22

    申请人： Samsung Electronics Co., Ltd.

    发明人： Jung, Sung-ouk ,  Huh, Nam ,  Lee, Soo-suk ,  Yoo, Chang-eun ,  Lee, Hun-joo

    IPC分类号： B01F13/02 ,  B01F13/00 ,  B01L3/00

    CPC分类号： B01L3/502738 ,  B01F13/0059 ,  B01F13/0222 ,  B01L3/50273 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0877

    摘要： Provided is an agitation device used to agitate a solution in a hybridization chamber, the agitation device including: the hybridization chamber (1); first and second air channels (2,2') connected to ends of the hybridization chamber; a first valve (3) disposed in the first air channel; a second valve (3') disposed in the second air channel; an integrated air channel (4) connecting the first and second air channels; and a pump (5) disposed in the integrated air channel. The agitation device is suitable for effective diffusion of a sample when performing hybridization using a DNA chip. Therefore, a probe can be effectively hybridized with a target material.

    摘要翻译： 提供了用于搅拌杂交室中的溶液的搅拌装置，所述搅拌装置包括：杂交室（1）; 连接到杂交室的端部的第一和第二空气通道（2,2'）; 设置在第一空气通道中的第一阀（3） 设置在第二空气通道中的第二阀（3'）; 连接第一和第二空气通道的集成空气通道（4） 以及设置在所述集成空气通道中的泵（5）。 搅拌装置适用于使用DNA芯片进行杂交时样品的有效扩散。 因此，探针可以有效地与靶材料杂交。