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    MONITORING TEMPERATURE WITH FLUORESCENCE
    1.
    发明公开
    MONITORING TEMPERATURE WITH FLUORESCENCE 审中-公开

    公开(公告)号：EP3379245A1

    公开(公告)日：2018-09-26

    申请号：EP18169448.0

    申请日：2013-10-08

    申请人： University of Utah Research Foundation

    发明人： SANFORD, Lindsay N ,  WITTWER, Carl T

    IPC分类号： G01N33/00

    CPC分类号： C12Q3/00 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/18 ,  C12Q1/686 ,  G01K11/20 ,  G01K11/32 ,  G01N21/6428 ,  G01N2021/6439

    摘要： 1. The application describes a method of calibrating a sample heating device, comprising:
    providing a sample that includes a temperature-sensitive reagent that emits a luminescent signal in response to a stimulus;
    wherein the luminescent signal emitted from the temperature-sensitive reagent changes as a function of temperature in a known and predictable manner and is not directly proportional to an amount of nucleic acid present in the sample,
    stimulating the temperature-sensitive reagent to induce emission of the luminescent signal therefrom;
    determining a luminescence-determined temperature of the sample based on the luminescent signal emitted by the temperature-sensitive reagent;
    determining a device-determined temperature for the sample; and
    adjusting a temperature setting of the sample heating device based on at least one of the luminescence-determined temperature and device-determined temperature.

    IMMOBILIZATION OF CELLS OR VIRUS PARTICLES ON PROTEIN STRUCTURES USING A MICROFLUIDIC CHAMBER
    5.
    发明公开
    IMMOBILIZATION OF CELLS OR VIRUS PARTICLES ON PROTEIN STRUCTURES USING A MICROFLUIDIC CHAMBER 有权
    用微流控芯室固定蛋白质结构上的细胞或病毒颗粒

    公开(公告)号：EP3256572A1

    公开(公告)日：2017-12-20

    申请号：EP16704539.2

    申请日：2016-02-05

    申请人： Karlsruher Institut für Technologie (KIT) ,  Universitätsklinikum Hamburg-Eppendorf (UKE)

    发明人： HIRTZ, Michael ,  FUCHS, Harald ,  PANTEL, Klaus ,  BRINKMANN, Falko

    IPC分类号： C12N7/02 ,  G01N33/569

    CPC分类号： G01N33/57492 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2300/0819 ,  B01L2300/18 ,  B01L2400/0406 ,  C12N7/02 ,  G01N33/56966 ,  G01N33/56983 ,  G01N2333/705 ,  G01N2469/10

    摘要： The present invention relates to methods for the immobilization of cells or virus particles of interest expressing one or more predetermined surface marker(s) on defined spots on a solid support, comprising the steps of providing a biological fluid sample suspected of containing cells or virus particles of interest; labeling the cells or virus particles of interest with antibodies, antibody fragments or antibody mimetics directed against the one or more predetermined surface marker(s) and carrying a first binding agent; and contacting the labeled cells or virus particles of interest with a solid support (biochip), said solid support comprising an array of defined isolated spots of a solid support-bound second binding agent, wherein the first and second binding agents can bind to each other. The present invention further relates to devices for the isolation of cells or virus particles of interest expressing one or more predetermined surface marker(s), comprising a microfluidic chamber comprising a solid support comprising an array of defined isolated spots of a solid support-bound binding agent, and a surface opposing the solid support, said surface having transverse structures that facilitate chaotic mixing of the fluid containing the cells or virus particles of interest when said fluid flows along said surface; and a capillary micropipette.