PIPETTE UND VERFAHREN ZUM PIPETTIEREN
    6.
    发明公开
    PIPETTE UND VERFAHREN ZUM PIPETTIEREN 有权
    移液器和方法移液

    公开(公告)号:EP2919911A1

    公开(公告)日:2015-09-23

    申请号:EP13791935.3

    申请日:2013-11-08

    IPC分类号: B01L3/02 B05B11/00

    摘要: A pipette (1) having a first chamber (3) arranged in a housing (2) and a second chamber (4) which adjoins it and extends via an outlet channel (8) to a pipette tip (6), said chambers being separated from one another by a longitudinally movable piston (5) having a piston non-return valve (19), and having a chamber non-return valve (9) for the second chamber (4) upstream of the outlet channel (8), wherein the first chamber (3) can be filled with liquid via a direct inlet (15), and a method for pipetting a liquid. The pipette (1) is characterized in that the first chamber (3) is connected via the inlet (15) and an outlet (16) to a recirculation circuit (17) of a reservoir vessel containing liquid to be pipetted or of an ultrapure water system (18). The process is characterized in that the liquid in the first chamber is recirculated via the recirculation circuit (17) of the reservoir vessel or of the ultrapure water system (18) and in that the following steps are conducted: a) filling the first chamber (3) with liquid via a direct inlet (15), b) filling the second chamber (4) with the liquid from the first chamber (3) by pulling back the piston (5) in the fill position in the direction of the first chamber (3) with an open piston non-return valve (19) and a closed chamber non-return valve (9) and c) emptying the second chamber (4) by driving the piston (5) in the emptying position in the direction of the outlet channel (8) remote from the first chamber (3) with a closed piston non-return valve (19) and an open chamber non-return valve (9).