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    MEANS AND METHODS FOR IDENTIFYING RIBOSOME ASSOCIATED RNA MOLECULES
    4.
    发明公开
    MEANS AND METHODS FOR IDENTIFYING RIBOSOME ASSOCIATED RNA MOLECULES 审中-公开
    MITTEL UND VERFAHREN ZUR IDENTIFIZIERUNG VON RIBOSOM-ASSOZIIERTEN RNA-MOLEKÜLEN

    公开(公告)号：EP2917363A1

    公开(公告)日：2015-09-16

    申请号：EP13786285.0

    申请日：2013-11-06

    申请人： Gerber, André ,  Nairz, Knud

    发明人： Gerber, André ,  Nairz, Knud

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q2522/101 ,  C12Q2535/122 ,  C12Q2563/131 ,  C12Q2563/149 ,  C12Q2565/518

    摘要： The present invention relates to a method for identifying one or more RNA molecules, which are directly or indirectly associated with at least one ribosome, comprising: contacting a cellular extract with a molecule specifically binding to a ribosomal protein or a group of ribosomal proteins, wherein said specifically binding molecule is immobilized on a substrate, matrix or bead; and identifying one or more RNA molecules, which are directly or indirectly associated with at least one ribosome comprising said ribosomal protein or group of ribosomal proteins. The present invention further relates to a method for identifying one or more RNA molecules, which are differentially associated with at least one ribosome, comprising: contacting a cellular extract which is derived from cells grown under specific conditions, with a molecule specifically binding to a ribosomal protein or a group of ribosomal proteins; contacting a cellular extract derived from cells grown under normal conditions, with a molecule specifically binding to a ribosomal protein or a group of ribosomal proteins; and identifying RNA molecules present under the specific conditions, but not normal conditions, or vice versa. Furthermore, the present invention relates to a corresponding kit for detecting in a cellular extract one or more RNA molecules, which are directly or indirectly associated with at least one ribosome.

    摘要翻译： 本发明涉及用于鉴定与至少一个核糖体直接或间接相关的一个或多个RNA分子的方法，其包括：使细胞提取物与特异性结合核糖体蛋白质或核糖体蛋白质组的分子接触，其中 所述特异性结合分子固定在基质，基质或珠粒上; 以及鉴定与包含所述核糖体蛋白质或核糖体蛋白质组的至少一个核糖体直接或间接相关的一个或多个RNA分子。 本发明还涉及用于鉴定与至少一个核糖体差异相关的一种或多种RNA分子的方法，其包括：将衍生自在特定条件下生长的细胞的细胞提取物与特异性结合核糖体的分子接触 蛋白质或一组核糖体蛋白质; 使来自在正常条件下生长的细胞的细胞提取物与特异性结合核糖体蛋白质或核糖体蛋白质组的分子接触; 并鉴定存在于特定条件下但不是正常条件的RNA分子，反之亦然。 此外，本发明涉及用于在细胞提取物中检测与至少一个核糖体直接或间接相关的一个或多个RNA分子的相应试剂盒。