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公开(公告)号：EP1212438A1
公开(公告)日：2002-06-12
申请号：EP00954241.6
申请日：2000-08-25
CPC分类号： C07K14/415 , C12N15/8234
摘要： Novel methods for the expression of non-native genes in flax seeds and the seeds of other plant species are provided. The methods involve the use of seed-specific promoters obtained from flax. Additionally provided are novel flax seed-specific promoters, chimeric nucleic acid constructs comprising novel flax seed-specific promoters, transgenic plant cells, transgenic plants and transgenic plant seeds containing novel flax seed-specific promoters. The promoters and methods are useful, for example, for altering the seed oil and protein composition in flax seed or other plant seeds.
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公开(公告)号：EP1212438B1
公开(公告)日：2006-05-17
申请号：EP00954241.6
申请日：2000-08-25
CPC分类号： C07K14/415 , C12N15/8234
摘要： Novel methods for the expression of non-native genes in flax seeds and the seeds of other plant species are provided. The methods involve the use of seed-specific promoters obtained from flax. Additionally provided are novel flax seed-specific promoters, chimeric nucleic acid constructs comprising novel flax seed-specific promoters, transgenic plant cells, transgenic plants and transgenic plant seeds containing novel flax seed-specific promoters. The promoters and methods are useful, for example, for altering the seed oil and protein composition in flax seed or other plant seeds.
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3.发明公开
公开(公告)号：EP1346056A1
公开(公告)日：2003-09-24
申请号：EP01992353.1
申请日：2001-12-19
发明人： VAN ROOIJEN, Gijs , DECKERS, Harm , HEIFETZ, Peter, Bernard , BRIGGS, Steven, P. , DALMIA, Bipin, Kumar , DEL VAL, Gregg , ZAPLACHINSKI, Steve , MOLONEY, Maurice
IPC分类号： C12N15/79
CPC分类号： C12N15/8258 , C12N15/8257
摘要： Improved methods for the production of multimeric-protein-complexes, such as redox proteins and immunoglobins, in association with oil bodies are described. The redox protein is enzymatically active when prepared in association with the oil bodies. Also provided are related nucleic acids, proteins, cells, plants, and compositions.
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公开(公告)号：EP1633876B1
公开(公告)日：2008-08-20
申请号：EP04737835.1
申请日：2004-06-17
CPC分类号： C07K14/62 , C12N15/8257
摘要： Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5’ supl; to 3’ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.
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公开(公告)号：EP1007554A1
公开(公告)日：2000-06-14
申请号：EP97946991.3
申请日：1997-12-05
CPC分类号： C07H21/00 , B01D15/08 , B01D15/3804 , B01D15/3809 , C01G11/003 , C07K1/22 , C07K14/415 , C07K16/065 , C08B1/00 , C12N9/6429 , C12Y304/21005
摘要： A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.
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公开(公告)号：EP0977873B1
公开(公告)日：2006-10-04
申请号：EP98916746.5
申请日：1998-04-23
IPC分类号： C12N15/62 , C12N15/15 , C07K14/815 , C07K14/61 , C12N9/50 , C12N15/57 , A61K38/58 , A61K38/27 , A23L1/305
CPC分类号： C12N15/62 , A23L33/17 , C07K14/61 , C07K14/815 , C07K2319/00 , C07K2319/02 , C07K2319/21 , C07K2319/50 , C07K2319/75 , C12N9/50
摘要： An improved method for recovering recombinantly produced polypeptides is described. The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. The pro-peptide-polypeptide fusion protein can be cleaved and the recombinant polypeptide released under the appropriate conditions.
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公开(公告)号：EP1007554B1
公开(公告)日：2006-03-29
申请号：EP97946991.3
申请日：1997-12-05
CPC分类号： C07H21/00 , B01D15/08 , B01D15/3804 , B01D15/3809 , C01G11/003 , C07K1/22 , C07K14/415 , C07K16/065 , C08B1/00 , C12N9/6429 , C12Y304/21005
摘要： A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.
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公开(公告)号：EP1212438B8
公开(公告)日：2007-02-21
申请号：EP00954241.6
申请日：2000-08-25
CPC分类号： C07K14/415 , C12N15/8234
摘要： Novel methods for the expression of non-native genes in flax seeds and the seeds of other plant species are provided. The methods involve the use of seed-specific promoters obtained from flax. Additionally provided are novel flax seed-specific promoters, chimeric nucleic acid constructs comprising novel flax seed-specific promoters, transgenic plant cells, transgenic plants and transgenic plant seeds containing novel flax seed-specific promoters. The promoters and methods are useful, for example, for altering the seed oil and protein composition in flax seed or other plant seeds.
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公开(公告)号：EP1216306A1
公开(公告)日：2002-06-26
申请号：EP00954228.3
申请日：2000-08-23
CPC分类号： C12N9/6483 , C12N15/8257 , C12Y304/23004
摘要： A method for the commercial production of chymosin which involves the recombinant expression of chymosin in plant seeds, that enables chymosin to accumulate to at least 0,5 % (w/w) of total seed protein is described. An improved method for the isolation of chymosin from the seed is also described.
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公开(公告)号：EP0977873A1
公开(公告)日：2000-02-09
申请号：EP98916746.5
申请日：1998-04-23
IPC分类号： C12N15/62 , C12N15/15 , C07K14/815 , C07K14/61 , C12N9/50 , C12N15/57 , A61K38/58 , A61K38/27 , A23L1/305
CPC分类号： C12N15/62 , A23L33/17 , C07K14/61 , C07K14/815 , C07K2319/00 , C07K2319/02 , C07K2319/21 , C07K2319/50 , C07K2319/75 , C12N9/50
摘要： An improved method for recovering recombinantly produced polypeptides is described. The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. The pro-peptide-polypeptide fusion protein can be cleaved and the recombinant polypeptide released under the appropriate conditions.
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