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    IMPROVED SELEX AND PHOTOSELEX
    4.
    发明公开
    IMPROVED SELEX AND PHOTOSELEX 审中-公开
    改进的SELEX和PHOTOSELEX

    公开(公告)号：EP2069496A1

    公开(公告)日：2009-06-17

    申请号：EP08781998.3

    申请日：2008-07-17

    申请人： Somalogic, Inc.

    发明人： SCHNEIDER, Daniel J. ,  WILCOX, Sheri K. ,  ZICHI, Dominic ,  NIEUWLANDT, Dan ,  CARTER, Jeff ,  GOLD, Larry

    IPC分类号： C12N15/10 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

    摘要： The present disclosure describes improved SELEX methods for generating nucleic acid ligands that are capable of binding to target molecules and improved photoSELEX methods for generating photoreactive nucleic acid ligands that are capable of both binding and covalently crosslinking to target molecules. The disclosure further describes nucleic acid libraries having expanded physical and chemical properties and their use in SELEX and photoSELEX; methods for increasing the crosslinking efficiencies of photoaptamers; methods for producing photoaptamers having selective modifications that enhance functionality and minimize non-specific photoreactions; and methods for generating truncated nucleic acid ligands from nucleic acid ligands of longer length. The disclosure further describes aptamers and photoaptamers obtained by using any of the foregoing.

    摘要翻译： 本公开描述了用于产生能够结合靶分子的核酸配体的改进SELEX方法和用于产生能够结合和共价交联靶分子的光反应性核酸配体的改进的photoSELEX方法。 本公开进一步描述了具有扩大的物理和化学性质的核酸文库及其在SELEX和photoSELEX中的用途; 提高光适体的交联效率的方法; 用于生产具有选择性修饰的光适体的方法，所述修饰提高功能并使非特异性光反应最小化; 以及由更长长度的核酸配体产生截短的核酸配体的方法。 本公开进一步描述了通过使用任何前述获得的适体和光适体。

    METHOD FOR GENERATING APTAMERS WITH IMPROVED OFF-RATES
    7.
    发明公开
    METHOD FOR GENERATING APTAMERS WITH IMPROVED OFF-RATES 审中-公开
    产生具有改进失速率的APTAMER的方法

    公开(公告)号：EP3284832A2

    公开(公告)日：2018-02-21

    申请号：EP17181688.7

    申请日：2008-07-17

    申请人： Somalogic, Inc.

    发明人： ZICHI, Dominic ,  WILCOX, Sheri K. ,  BOCK, Chris ,  SCHNEIDER, Daniel J. ,  EATON, Bruce ,  GOLD, Larry

    IPC分类号： C12Q1/68 ,  C12N15/115 ,  C12Q1/6811 ,  C12N15/10 ,  C12N15/11

    CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

    摘要： The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently cross linking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

    摘要翻译： 本公开描述了用于产生能够结合靶分子的适体的改进的SELEX方法以及用于产生能够结合和共价交联至靶分子的光反应性适体的改进的photoSELEX方法。 具体而言，本公开描述了产生具有比使用现有SELEX和photoSELEX方法获得的更慢的解离速率常数的适体和光适体的方法。 本公开进一步描述了具有比使用现有方法获得的更慢的解离速率常数的适体和光适体。 另外，本公开描述了包含多种功能性的适体构建体，包括可切割元件，检测元件和捕获或固定元件。