公开(公告)号：EP3660036A1

公开(公告)日：2020-06-03

申请号：EP18838960.5

申请日：2018-07-25

申请人： Spiber Inc. ,  Riken

发明人： MORITA Keisuke ,  ABE Yunosuke ,  MAEKAWA Takehisa ,  KOTAKA Koichi ,  SUGAHARA Junichi ,  NUMATA Keiji

IPC分类号： C07K14/435 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/12 ,  D01F4/02

摘要： The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A) n motif-REP] m or Formula 2: [(A) n motif-REP] m -(A) n motif, in which the domain sequence has an amino acid sequence with a reduced content of a glutamine residue equivalent to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin. [In Formula 1 and Formula 2, (A) n motif represents an amino acid sequence consisting of 4 to 27 amino acid residues, and the number of alanine residues with respect to the total number of amino acid residues in the (A) n motif is 80% or more. REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 10 to 300. A plurality of (A) n motifs may be the same amino acid sequence or different amino acid sequences.]

公开(公告)号：EP3508517A1

公开(公告)日：2019-07-10

申请号：EP17846701.5

申请日：2017-09-01

申请人： Spiber Inc. ,  Tekunohama Co., Ltd.

发明人： HIRAI Shinji ,  UBUKATA Yukimasa ,  ABE Ayumi ,  KOTAKA Koichi ,  NAKAYAMA Yuki ,  NOBA Junichi

IPC分类号： C08J5/00 ,  B29C43/02 ,  C07K14/435 ,  C08H1/00 ,  C08L89/00

摘要： The present invention provides a method for producing a molded article including a step 1 of adding water to a composition including a structural protein, a step 2 of molding a water-containing composition obtained in the step 1, and a step 3 of drying the molded composition obtained in the step 2 to obtain the molded article.

公开(公告)号：EP3564254A1

公开(公告)日：2019-11-06

申请号：EP17889372.3

申请日：2017-12-25

申请人： Spiber Inc.

发明人： SEKIYAMA Kazuhide ,  OZEKI Akihiko ,  OKADA Ryoji ,  KOTAKA Koichi

IPC分类号： C07K1/14 ,  C08J11/08 ,  C12N15/09

摘要： The present invention relates to a method for recovering protein, which recovers a target protein from a mixture containing the target protein and a material different from the target protein, the method including a dissolution step of dissolving either the target protein or the material by applying pressure while heating to a solution for dissolution containing the mixture and a polar solvent, and a separation step of separating an obtained solution.

公开(公告)号：EP3922763A1

公开(公告)日：2021-12-15

申请号：EP20753263.1

申请日：2020-02-07

申请人： Spiber Inc.

发明人： ANDO Hirotada ,  ISHII Hideto ,  KOTAKA Koichi ,  SATO Ryoko ,  KANO Hiroshi ,  KOBAYASHI Jun ,  SATO Akito ,  ABE Yunosuke

IPC分类号： D01F4/00 ,  D01F4/02 ,  C07K14/435 ,  C12N15/12

摘要： An object of the present invention is to provide a method for producing an artificial structural protein fiber having a small diameter and having a stress equal to or higher than that of the related art. A method for producing an artificial structural protein fiber according to the present invention is a method for producing an artificial structural protein fiber by a wet spinning method, the method including a coagulation step of discharging a spinning dope containing an artificial structural protein and an organic solvent from a spinneret into a coagulation liquid to coagulate the artificial structural protein, wherein a bath draft in the coagulation step is more than 0.4 and 20 or less.

公开(公告)号：EP3922764A1

公开(公告)日：2021-12-15

申请号：EP20752918.1

申请日：2020-02-07

申请人： Spiber Inc.

发明人： ABE Yunosuke ,  ISHII Hideto ,  SATO Ryoko ,  SATO Akito ,  ANDO Hirotada ,  KANO Hiroshi ,  KOTAKA Koichi ,  KOBAYASHI Jun

IPC分类号： D01F4/02 ,  A61L17/08 ,  A61L27/22 ,  C07K14/435

摘要： An object of the present invention is to provide a method for producing a modified fibroin multifilament excellent in productivity and a multifilament produced by the same. The multifilament of the present invention contains modified fibroin, in which the multifilament has 100 or more constituent single yarns, a coefficient of variation in elastic modulus is 15% or less, a coefficient of variation in strength is 15% or less, and a coefficient of variation in elongation is less than 33%.

公开(公告)号：EP3385305A1

公开(公告)日：2018-10-10

申请号：EP16870659.6

申请日：2016-11-29

申请人： Spiber Inc. ,  Kojima Industries Corporation ,  Tekunohama Co., Ltd.

发明人： KOTAKA Koichi ,  ISHII Takaoki ,  SUGAHARA Junichi ,  SATO Ryota ,  NOBA Junichi

IPC分类号： C08J3/02 ,  C07K1/14 ,  C07K14/435 ,  C08L89/00

CPC分类号： C08J3/02 ,  C07K1/14 ,  C07K14/435 ,  C07K14/43586 ,  C08L89/00

摘要： Provided is a method for producing a protein solution, including applying a pressure to a dispersion liquid containing a protein and a polar solvent in which the protein is dispersed, to obtain a protein solution containing the protein and the polar solvent in which the protein is dissolved.