公开(公告)号：EP2207899B1

公开(公告)日：2018-04-18

申请号：EP08838436.7

申请日：2008-10-07

申请人： Synthetic Genomics, Inc.

发明人： GIBSON, Daniel, G. ,  BENDERS, Gwynedd, A. ,  YOUNG, Lei ,  GLASS, John, I. ,  VENTER, J., Craig ,  HUTCHISON, Clyde, A. ,  SMITH, Hamilton, O.

IPC分类号： C12Q1/68

CPC分类号： C12N15/1093 ,  C12N15/81

摘要： A method to assemble any desired nucleic acid molecule by combining cassettes in vitro to form assemblies which are further combined in vivo, or by assembling large numbers of DNA fragments by recombination in a yeast culture to obtain desired DNA molecules of substantial size is described.

公开(公告)号：EP2403944B1

公开(公告)日：2017-06-28

申请号：EP10708854.4

申请日：2010-03-05

申请人： Synthetic Genomics, Inc.

发明人： BENDERS, Gwynedd, A. ,  GLASS, John, I. ,  HUTCHISON, Clyde, A. ,  LARTIGUE, Carole ,  VASHEE, Sanjay ,  ALGIRE, Mikkel, A. ,  SMITH, Hamilton, O. ,  MERRYMAN, Charles, E. ,  NOSKOV, Vladimir, N. ,  CHUANG, Ray-Yuan ,  GIBSON, Daniel G. ,  VENTER, J., Craig

IPC分类号： C12N15/10

CPC分类号： C12N15/905 ,  C12N15/10 ,  C12N15/1031 ,  C12N15/1079 ,  C12N15/1093 ,  C12N15/81

公开(公告)号：EP2542676A1

公开(公告)日：2013-01-09

申请号：EP10726723.9

申请日：2010-05-19

申请人： Synthetic Genomics, Inc.

发明人： BENDERS, Gwynedd, A. ,  GLASS, John, I. ,  HUTCHISON, Clyde, A. ,  LARTIGUE, Carole ,  VASHEE, Sanjay ,  ALGIRE, Mikkel, A. ,  SMITH, Hamilton, O. ,  MERRYMAN, Charles, E. ,  NOSKOV, Vladimir, N. ,  CHUANG, Ray-yuan ,  GIBSON, Daniel, G. ,  VENTER, J. Craig

IPC分类号： C12N15/10 ,  C12N15/74

CPC分类号： C12N15/1079 ,  C12N15/1031 ,  C12N15/66 ,  C12N15/81

摘要： Compositions and methods are disclosed herein for cloning a synthetic or a semi-synthetic donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.

公开(公告)号：EP2403944A2

公开(公告)日：2012-01-11

申请号：EP10708854.4

申请日：2010-03-05

申请人： Synthetic Genomics, Inc.

发明人： BENDERS, Gwynedd, A. ,  GLASS, John, I. ,  HUTCHISON, Clyde, A. ,  LARTIGUE, Carole ,  VASHEE, Sanjay ,  ALGIRE, Mikkel, A. ,  SMITH, Hamilton, O. ,  MERRYMAN, Charles, E. ,  NOSKOV, Vladimir, N. ,  CHUANG, Ray-Yuan ,  GIBSON, Daniel G. ,  VENTER, J., Craig

IPC分类号： C12N15/10

CPC分类号： C12N15/905 ,  C12N15/10 ,  C12N15/1031 ,  C12N15/1079 ,  C12N15/1093 ,  C12N15/81

摘要： Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.

公开(公告)号：EP2890787B1

公开(公告)日：2019-05-01

申请号：EP13833688.8

申请日：2013-08-30

申请人： Synthetic Genomics, Inc.

发明人： KARAS, Bogumil, J. ,  HUTCHISON, Clyde, A. ,  SMITH, Hamilton, O. ,  SUZUKI, Yo

IPC分类号： C12N15/87 ,  C12N15/81

公开(公告)号：EP2890787A2

公开(公告)日：2015-07-08

申请号：EP13833688.8

申请日：2013-08-30

申请人： Synthetic Genomics, Inc.

发明人： KARAS, Bogumil, J. ,  HUTCHISON, Clyde, A. ,  SMITH, Hamilton, O. ,  SUZUKI, Yo

IPC分类号： C12N15/04

CPC分类号： C12N15/81 ,  C12N15/87

摘要： The presently disclosed invention relates to methods of transferring large nucleic acid molecules or a genome from one cell (the donor) into heterologous host cells in the presence of a crowding agent. The method allows for greater ease and efficiency of transfer of genetic material. Introduction of the donor genetic material into the recipient host cells also allows for manipulation of the donor nucleic acid molecule or genome within the host cells. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.

公开(公告)号：EP2207899A2

公开(公告)日：2010-07-21

申请号：EP08838436.7

申请日：2008-10-07

申请人： Synthetic Genomics, Inc.

发明人： GIBSON, Daniel, G. ,  BENDERS, Gwynedd, A. ,  YOUNG, Lei ,  GLASS, John, I. ,  VENTER, J., Craig ,  HUTCHISON, Clyde, A. ,  SMITH, Hamilton, O.

IPC分类号： C12Q1/68

CPC分类号： C12N15/1093 ,  C12N15/81

摘要： A method to assemble any desired nucleic acid molecule by combining cassettes in vitro to form assemblies which are further combined in vivo, or by assembling large numbers of DNA fragments by recombination in a yeast culture to obtain desired DNA molecules of substantial size is described.