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公开(公告)号：EP1163912A1
公开(公告)日：2001-12-19
申请号：EP00908055.7
申请日：2000-03-14
发明人： KATO, Ikunoshin , ASADA, Kiyozo , UENO, Mitsuhiro , HASHINO, Kimikazu , YOSHIOKA, Hirofumi, 311, Hamoparesu-Kusatsu , TANAKA, Keiji
IPC分类号： A61K48/00
CPC分类号： A61K48/00 , C12N15/86 , C12N2710/10043 , C12N2740/13043 , C12N2810/40
摘要： Gene therapeutics to be used in treating diseases showing sensitivity to gene therapy, characterized by containing as the active ingredient an efficacious amount of a functional substance which has a function of having an affinity for a virus containing a gene usable in the gene therapy and another function of having an affinity specific for a target cell with a need for the gene transfer, or an efficacious amount of a functional substance which has an affinity for the above virus and an efficacious amount of another functional substance which has an affinity specific for the above cell.
摘要翻译： 用于治疗对基因治疗具有敏感性的疾病的基因疗法，其特征在于，含有作为活性成分的有效量的具有对含有可用于基因治疗的基因的病毒具有亲和力的功能物质和另一功能 对具有基因转移需要的靶细胞具有亲和力的有效量的对上述病毒具有亲和力的有效量的功能物质和对上述细胞具有亲和力的有效量的另一种功能性物质 。
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2.发明公开METHOD FOR IMMOBILIZING DNA ON A CARRIER 审中-公开VERFAHREN ZUR IMMOBILISIERUNG VON DNS AUF EINEMTRÄGER
公开(公告)号：EP1138761A1
公开(公告)日：2001-10-04
申请号：EP99959690.1
申请日：1999-12-08
发明人： TAKAYAMA, Masanori , ROKUSHIMA, Masatomo , UEDA, Minoru 412, Hamoparesu-Kusatsu , OKAMOTO, Sachiko , OZAKI, Aya , MINENO, Junichi , KIMIZUKA, Fusao , ASADA, Kiyozo , KATO, Ikunoshin
CPC分类号： C12Q1/6834 , C12N15/1006
摘要： A method for immobilizing DNA on a carrier characterized by having the step of bringing the DNA into contact with the carrier in a buffer containing one or more members selected from the group consisting of morpholine, morpholine derivatives, salts thereof and carbonates thereof.
摘要翻译： 一种将DNA固定在载体上的方法，其特征在于具有使DNA与载体接触的步骤，该缓冲液含有选自吗啉，吗啉衍生物，其盐和碳酸酯的一种或多种成员。
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公开(公告)号：EP1094114A1
公开(公告)日：2001-04-25
申请号：EP99926821.2
申请日：1999-06-25
发明人： UENO, Mitsuhiro , YOSHIOKA, Hirofumi , KONISHI, Haruko 407, Gurankoto-Rakuyo , HASHINO, Kimikazu , MORISHITA, Mio , CHONO, Hideto , MIYAMURA, Tsuyoshi , SANO, Mutsumi , ASADA, Kiyozo , FUJINAGA, Kei , KATO, Ikunoshin
CPC分类号： C12N15/86 , A61K48/00 , C12N2740/13043 , C12N2740/13045 , C12N2810/10 , C12N2810/80 , C12N2810/851 , C12N2810/859
摘要： Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferrin receptor, or introducing a new functional group into the functional substance.
摘要翻译： 通过使用逆转录病毒将基因转移到靶细胞中的改进方法，其中基因转移效率得到改善，并且通过将逆转录病毒结合到固定在载体上并具有结合逆转录病毒活性的功能物质来有效转化靶细胞 随后洗涤; 使用能够特异性识别细胞的抗体，层粘连蛋白或富甘糖型糖链作为具有与靶细胞结合的活性的物质; 预处理靶细胞以使转铁蛋白受体失活，或将新的官能团引入功能物质。
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公开(公告)号：EP1196539A1
公开(公告)日：2002-04-17
申请号：EP00947091.5
申请日：2000-07-06
发明人： KATO, Ikunoshin , ASADA, Kiyozo , TAKAYAMA, Masanori , WATT, Mary-Anne , BUDKER, Vladimir,G , SLATTUM, Paul, M , HAGSTOROM, James E. , WOLFF, Jon, A
CPC分类号： C40B50/18 , B01J2219/00529 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , C03C17/3405 , C07H21/00 , C12Q1/6834 , C40B40/06
摘要： Disclosed is a substrate and process for nucleic acid immobilization on a solid support. A polyanion containing an activated ester is covalently attached to a solid support. In one preferred embodiment, the polyanion is polyacrylic acid.
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5.发明公开METHOD FOR AMPLIFYING NUCLEIC ACID SEQUENCE 有权Verfahren zur Amplifizierung einerNukleinsäuresequenzunter Verwendung eineschimärenPrimers
公开(公告)号：EP1167524A1
公开(公告)日：2002-01-02
申请号：EP00908056.5
申请日：2000-03-14
发明人： MUKAI, Hiroyuki , YAMAMOTO, Junko , TAKEDA, Osamu , MIYAKE, Kazue , UEMORI, Takashi , SATO, Yoshimi , MORIYAMA, Mariko , SAWARAGI, Haruhisa , HAGIYA, Michio , ASADA, Kiyozo , KATO, Ikunoshin
CPC分类号： C12Q1/6844 , C12Q2531/119 , C12Q2525/121 , C12Q2521/319
摘要： A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
摘要翻译： 一种用于扩增核酸序列的方法和方法，其特征在于在嵌合寡核苷酸引物存在下进行DNA合成反应; 提供大量DNA扩增片段的方法; 通过将上述方法与另一种核酸序列扩增方法结合来扩增核酸序列的有效方法; 用于检测用于检测或定量微生物如病毒，细菌，真菌或酵母的核酸序列的方法; 以及通过上述方法原位检测DNA扩增片段的方法。
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公开(公告)号：EP1142992A1
公开(公告)日：2001-10-10
申请号：EP99959804.8
申请日：1999-12-14
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01091
摘要： Polypeptides having the amino acid sequence represented by SEQ ID NO:1 or derived therefrom by at least one of deletion, addition, insertion or substitution of one or more amino acids in the above sequence and showing a cellobiohydrolase activity.
摘要翻译： 具有由SEQ ID NO：1表示的氨基酸序列或通过上述序列中的一个或多个氨基酸的缺失，添加，插入或取代中的至少一种而显示出纤维二糖水解酶活性的多肽。
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公开(公告)号：EP1138762A1
公开(公告)日：2001-10-04
申请号：EP99959692.7
申请日：1999-12-08
发明人： UEDA, Minoru, 412, Hamoparesu-Kusatsu , OKAMOTO, Sachiko , OZAKI, Aya , MINENO, Junichi , KIMIZUKA, Fusao , ASADA, Kiyozo , KATO, Ikunoshin
CPC分类号： C12Q1/6834 , C12N15/1006
摘要： A method for immobilizing an oligonucleotide on a carrier by spotting a buffer containing the oligonucleotide onto the carrier, characterized in that the oligonucleotide is immobilized on the carrier via a covalent bond.
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8.发明公开
公开(公告)号：EP1111044A1
公开(公告)日：2001-06-27
申请号：EP99940673.9
申请日：1999-09-06
发明人： UEMORI, Takashi , SATO, Yoshimi , OKAWA, Mariko , FUJITA, Tomoko , MIYAKE, Kazue , TAKEDA, Osamu , SAGAWA, Hiroaki , HAGIYA, Michio , MUKAI, Hiroyuki , ASADA, Kiyozo , KATO, Ikunoshin
CPC分类号： C12N15/1003 , C12Q1/686 , C12Q2527/125
摘要： A DNA synthesis method with a shortened time period required for DNA synthesis by polymerase chain reaction (PCR), characterized in that a DNA polymerase is used in an amount effective for providing more than 10 ng of amplified DNA fragments of about 2 kb per 50 µl of a reaction mixture, when PCR is carried out under the following conditions (A) and (B): (A) reaction mixture: 50 µl volume of a reaction mixture comprising DNA polymerase, 1 ng of genomic DNA from Escherichia coli, and 10 pmol each of primers Eco-1 and Eco-2 (nucleotide sequences of the primers Eco-1 and Eco-2 being shown in SEQ ID NOs: 10 and 11 of Sequence Listing, respectively); and having a composition suitable for the DNA polymerase; and (B) reaction conditions: 35 cycles of PCR, wherein one cycle consists of 99°C, 1 second - 66°C, 7 seconds; a kit for DNA synthesis usable for the DNA synthesis method; and an article of manufacture of a PCR agent. According to the present invention, the procedures in the genetic engineering studies and industries involved with PCR can be speeded up.
摘要翻译： 一种通过聚合酶链式反应（PCR）进行DNA合成所需的时间缩短的DNA合成方法，其特征在于使用DNA聚合酶，其用量可提供超过10ng的扩增DNA片段，每50ul约2kb （A）和（B）：（A）反应混合物：50μl体积的包含DNA聚合酶的反应混合物，1ng来自大肠杆菌的基因组DNA， 和引物Eco-1和Eco-2（引物Eco-1和Eco-2的核苷酸序列分别示于序列表的SEQ ID NO：10和11中）的10pmol; 并具有适合于DNA聚合酶的组合物; 和（B）反应条件：35个循环的PCR，其中一个循环由99℃，1秒-66℃，7秒; 用于DNA合成方法的DNA合成试剂盒; 和PCR试剂的制造。 根据本发明，可以加快基因工程研究中涉及PCR的工艺步骤。
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9.发明公开SYSTEM FOR EXPRESSING HYPERTHERMOSTABLE PROTEASE 失效SYSTEM ZUM EXPRIMIEREN EINES HYPERTHERMOSTABILES PROTEIN
公开(公告)号：EP0994191A1
公开(公告)日：2000-04-19
申请号：EP98923114.7
申请日：1998-06-04
CPC分类号： C12N15/75 , C07K2319/02 , C12N9/52
摘要： A hyperthermostable protease having the amino acid sequence represented by the SEQ ID NO:1 of the Sequence Listing or a sequence derived therefrom by deletion, substitution, insertion or addition of one to several amino acid residues, a gene encoding the hyperthermostable protease, and a process for preparing the protease, aiming at providing by genetic engineering techniques a hyperthermophile protease which is advantageous for industrial use.
摘要翻译： 具有由序列表的SEQ ID NO：1表示的氨基酸序列或通过缺失，取代，插入或添加1〜数个氨基酸残基而得到的氨基酸序列的超热稳定蛋白酶，编码超热稳定蛋白酶的基因，以及 旨在通过基因工程技术提供有利于工业用途的超嗜热蛋白酶的制备蛋白酶的方法。
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公开(公告)号：EP0930361A1
公开(公告)日：1999-07-21
申请号：EP97928515.2
申请日：1997-06-30
IPC分类号： C12N5/00
CPC分类号： C12N5/0093 , C12N5/0647 , C12N2501/125 , C12N2501/23 , C12N2501/90
摘要： Cell compositions from which cancer cells have been selectively eliminated by using apoptosis inducers specific to cancer cells.
摘要翻译： 通过使用对癌细胞特异性的细胞凋亡诱导剂已经选择性地消除癌细胞的细胞组合物。
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