公开(公告)号：EP1224023B1

公开(公告)日：2005-05-18

申请号：EP00961540.2

申请日：2000-09-01

Applicant: THE CLEVELAND CLINIC FOUNDATION ,  THE OHIO STATE UNIVERSITY

Inventor： ZBOROWSKI, Maciej ,  CHALMERS, Jeff ,  MOORE, Lee, R.

IPC: B01D49/00 ,  B01D69/02 ,  B01D63/06

CPC classification number: B03C5/026 ,  B01D39/1692 ,  B01D39/18 ,  B01D49/00 ,  B01D57/02 ,  B01D61/00 ,  B01D61/14 ,  G01N27/44769 ,  G01N30/0005

Abstract: The present invention provides apparatuses and methods for continuously separating particles of interest from a heterogenous particle population. the heterogeneity can be based on, for example, magnetic susceptibility, particle size, thermal diffusion, phase solubility, and combinations of the preceding. Based on the heterogeneity, a separation force capable of exploiting the separand (i.e., particles subject to the separation process) is provided. The various embodiments of the present invention preferably employ an annular separation channel, appropriate separation force and flow compartments. In a first embodiment, an annular separation channel having semi-permeable inner and outer cylindrical walls is used to generate lateral convection forces. In a second embodiment of the present invention, an annular separation chanel having heat conductive inner and outer cylindrical walls is used to generate thermal diffusion forces. In a third embodiment, an annular separation channel having non-permeable inner and outer cylindrical walls is used to generate a solubility difference separation force based on solubility coefficient differences between phases of flow compartments. In a fourth embodiment, an annular separation channel having electrically conductive inner and outer cylindrical walls is used to generate an electrophoretic separation force. In a fifth embodiment, an annular separation channel having electrically conductive inner and outer cylindrical walls is used to generate a dielectrophoretic separation force. Yet other embodiments of the present invention provide for the combination of separation forces including lateral convection, thermal diffusion, solubility difference, electrophoretic, dielectrophoretic, and magnetic separation forces.

公开(公告)号：EP1434998A2

公开(公告)日：2004-07-07

申请号：EP01987193.8

申请日：2001-10-31

Applicant: The Cleveland Clinic Foundation ,  The Ohio State University

Inventor： FLEISCHMAN, Aaron, J. ,  ROY, Shuvo ,  CHALMERS, Jeff ,  ZBOROWSKI, Maciej

IPC: G01N33/553 ,  G01N33/53

CPC classification number: G01N33/54326 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/043 ,  B01L2400/0487 ,  C12M47/04 ,  G01N15/1031 ,  G01N15/1056 ,  G01N15/1456 ,  G01N2015/1006 ,  G01N2015/1062 ,  G01N2015/1081

Abstract: The present invention provides a MEMS-based integrated particle identification system having a substrate, a magnetic structure, and a bioferrograph. The substrate includes a topside portion, backside portion and a flow system. The flow system includes a flow channel for accepting the flow of a stream of particles to identified. The magnetic structure is in physical communication with the topside and backside portions of the substrate and has at least two pole pieces. A plurality of pole piece embodiments are provided for generating a magnetic field that acts on magnetically susceptible particles in the flow stream. The bioferrograph has at least one sensor for identifying the presence and quantity of magnetically susceptible particles. A plurality of sensor embodiments are also provided.

公开(公告)号：EP1224023A2

公开(公告)日：2002-07-24

申请号：EP00961540.2

申请日：2000-09-01

Applicant: THE CLEVELAND CLINIC FOUNDATION ,  THE OHIO STATE UNIVERSITY

Inventor： ZBOROWSKI, Maciej ,  CHALMERS, Jeff ,  MOORE, Lee, R.

IPC: B01D49/00 ,  B01D69/02 ,  B01D63/06

CPC classification number: B03C5/026 ,  B01D39/1692 ,  B01D39/18 ,  B01D49/00 ,  B01D57/02 ,  B01D61/00 ,  B01D61/14 ,  G01N27/44769 ,  G01N30/0005

Abstract: The present invention provides apparatuses and methods for continuously separating particles of interest from a heterogenous particle population. the heterogeneity can be based on, for example, magnetic susceptibility, particle size, thermal diffusion, phase solubility, and combinations of the preceding. Based on the heterogeneity, a separation force capable of exploiting the separand (i.e., particles subject to the separation process) is provided. The various embodiments of the present invention preferably employ an annular separation channel, appropriate separation force and flow compartments. In a first embodiment, an annular separation channel having semi-permeable inner and outer cylindrical walls is used to generate lateral convection forces. In a second embodiment of the present invention, an annular separation chanel having heat conductive inner and outer cylindrical walls is used to generate thermal diffusion forces. In a third embodiment, an annular separation channel having non-permeable inner and outer cylindrical walls is used to generate a solubility difference separation force based on solubility coefficient differences between phases of flow compartments. In a fourth embodiment, an annular separation channel having electrically conductive inner and outer cylindrical walls is used to generate an electrophoretic separation force. In a fifth embodiment, an annular separation channel having electrically conductive inner and outer cylindrical walls is used to generate a dielectrophoretic separation force. Yet other embodiments of the present invention provide for the combination of separation forces including lateral convection, thermal diffusion, solubility difference, electrophoretic, dielectrophoretic, and magnetic separation forces.

公开(公告)号：EP0975744A1

公开(公告)日：2000-02-02

申请号：EP98907614.6

申请日：1998-02-25

Applicant: THE CLEVELAND CLINIC FOUNDATION ,  THE OHIO STATE UNIVERSITY

Inventor： ZBOROWSKI, Maciej ,  CHALMERS, Jeff ,  MOORE, Lee, R.

IPC: C12N13/00 ,  C12N1/02 ,  C12N5/02 ,  C12M1/18

CPC classification number: G01N33/54326 ,  B03C1/035 ,  C12M47/04 ,  C12N13/00 ,  G01N2015/149

Abstract: A method and apparatus for the fraction sorting of cells is provided. The invention includes a dispensing device, aspirating device, flow channel and a magnetic assembly. A first fractional sorting device includes a dipole fractional cell sorter. The dipole fractional cell sorter includes large aspect ratio flow channel and a dipole magnetic assembly. A second fractional sorting device includes a quadrupole fractional cell sorter. The quadrupole fractional cell sorter includes an annular flow channel having inlet and outlet splitters, and a quadrupole magnetic assembly. The quadrupole magnetic assembly includes pole pieces having projecting members with convex surfaces and is in physical communication with the annular flow chamber. The method of the present invention provides a flow through fractional cell sorting process which is based on the application of a magnetic force to cells having a range of magnetic labeling densities.

Abstract translation: 提供了用于细胞部分分选的方法和设备。 本发明包括分配装置，抽吸装置，流动通道和磁性组件。 第一分部分拣装置包括偶极部分分拣机。 偶极分数单元分选器包括大纵横比流动通道和偶极磁性组件。 第二部分分选装置包括四极分数细胞分选机。 四极分数单元分选器包括具有入口和出口分流器的环形流动通道和四极磁性组件。 四极磁性组件包括具有凸面的突出部件并且与环形流动室物理连通的极片。 本发明的方法提供了流过分数细胞分选过程，其基于将磁力施加于具有一定范围的磁性标记密度的细胞。