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1.发明授权Gene expression unit comprising the promoter and the terminator as well as the gene of alkaline protease 失效由启动子，终止子和碱性蛋白酶基因的表达单元的基因的
公开(公告)号：EP0427385B1
公开(公告)日：1996-08-07
申请号：EP90309821.8
申请日：1990-09-07
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2.发明公开Gene expression unit comprising the promoter and the terminator as well as the gene of alkaline protease 失效Aus启动子，终止子和碱基碱性蛋白酶bestehende Genexpressionseinheit。
公开(公告)号：EP0427385A1
公开(公告)日：1991-05-15
申请号：EP90309821.8
申请日：1990-09-07
发明人： Murakami, Kohji, c/o Chuo Kenkyusho , Ishida, Yutaka, c/o Chuo Kenkyusho , Masaki, Atsushi, c/o Chuo Kenkyusho , Kawabe, Haruhide, c/o Chuo Kenkyusho , Arimura, Hirofumi, c/o Chuo Kenkyusho , Tatsumi, Hiroki , Murakami, Seiji , Nakano, Eiichi , Motai, Hiroshi
摘要： A novel alkaline protease promoter and alkaline protease terminator which each effectively function in a host-vector system of molds can be obtained from yellow koji mold. A gene expression unit comprising the promoter and terminator is used to construct an expression vector capable of expressing an useful substance in molds such as Aspergillus oryzae , Aspergillus niger , Aspergillus awamori , Aspergillus sojae , etc.
摘要翻译： 一种新型的碱性蛋白酶启动子和碱性蛋白酶终止子，其可以在黄色曲霉菌中得到，其在模具的宿主 - 载体系统中都有效。 包括启动子和终止子的基因表达单元用于构建能够在霉菌中表达有用物质的表达载体，例如米曲霉，黑曲霉，泡盛曲霉，大曲霉等。
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3.发明授权Bioluminescence reagent and method for quantitative determination of adenosine phosphate ester using the reagent 失效Bioluminiszenzreagens和方法，用于使用该试剂腺苷磷酸酯的定量测定
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公开(公告)号：EP0781851B1
公开(公告)日：2003-07-09
申请号：EP96120896.4
申请日：1996-12-27
申请人： KIKKOMAN CORPORATION
发明人： Sakakibara, Tatsuya , Murakami, Seiji , Hattori, Noriaki , Yajitate, Keiko , Watarai, Teruo , Nakajima, Motoo , Imai, Kazuhiro
IPC分类号： C12Q1/34
CPC分类号： C12Q1/66 , C12Q1/34 , G01N2333/978
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5.发明公开ATP eliminator and the process for determining biological cells 失效ATP-Eliminator und Verfahren zur Bestimmung von biologischen Zellen
公开(公告)号：EP0781851A2
公开(公告)日：1997-07-02
申请号：EP96120896.4
申请日：1996-12-27
申请人： KIKKOMAN CORPORATION
发明人： Sakakibara, Tasuya , Murakami, Seiji , Hattori, Noriaki , Yajitate, Keiko , Watarai, Teruo , Nakajima, Motoo , Imai, Kazuhiro
IPC分类号： C12Q1/34
CPC分类号： C12Q1/66 , C12Q1/34 , G01N2333/978
摘要： The present invention provides a process for eliminating effectively ATP in a sample, using adenosine phosphate deaminase alone or in combination with at least one enzyme selected from the group consisting of apyrase, alkaline phosphatase, acid phosphatase, hexokinase and adenosine triphosphatase, a process for determining biological cells contained in foods and beverages in convenient and precise manner in combination with bioluminescence method, and a reagent for the determination thereof.
摘要翻译： 本发明提供了一种使用单独使用磷酸腺苷脱氨酶或与选自腺苷磷酸酶，碱性磷酸酶，酸性磷酸酶，己糖激酶和腺苷三磷酸酶中的至少一种酶组合的样品中ATP有效消除的方法， 以生物发光法结合方便，精确地包含在食品和饮料中的生物细胞，以及用于确定的试剂。
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6.发明公开SAMPLE EXAMINING INSTRUMENT AND WIPING-OUT EXAMINING INSTRUMENT 审中-公开VORRICHTUNG ZUR PROBENANALYSE UND ZUM REINIGEN
公开(公告)号：EP1167968A1
公开(公告)日：2002-01-02
申请号：EP00912914.9
申请日：2000-03-29
申请人： KIKKOMAN CORPORATION
IPC分类号： G01N33/48
CPC分类号： B01L3/5029 , B01L3/5082 , B01L2400/0683 , G01N33/5304
摘要： A sample examining instrument comprising a test-tube-form measuring container storing therein a reaction reagent such as an emission reagent, and a reagent container inserted for burying into the upper opening of the measuring container, wherein sealants are pasted to the upper and lower portions of the reagent container and a reagent such as an extract liquid is sealed in the reagent container, whereby providing the sample examining instrument downsized due to the reagent container fitted into the measuring container.
摘要翻译： 一种试样检查仪，其特征在于，包括容纳有发射试剂的反应试剂的试管形状的测定容器和插入到所述测量容器的上部开口中的试剂容器，其中将密封剂粘贴到所述上部和下部 的试剂容器和诸如提取液的试剂被密封在试剂容器中，由此由于将试剂容器装配到测量容器中而使样品检查仪器小型化。
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公开(公告)号：EP0781851A3
公开(公告)日：1998-04-29
申请号：EP96120896.4
申请日：1996-12-27
申请人： KIKKOMAN CORPORATION
发明人： Sakakibara, Tasuya , Murakami, Seiji , Hattori, Noriaki , Yajitate, Keiko , Watarai, Teruo , Nakajima, Motoo , Imai, Kazuhiro
IPC分类号： C12Q1/34
CPC分类号： C12Q1/66 , C12Q1/34 , G01N2333/978
摘要： The present invention provides a process for eliminating effectively ATP in a sample, using adenosine phosphate deaminase alone or in combination with at least one enzyme selected from the group consisting of apyrase, alkaline phosphatase, acid phosphatase, hexokinase and adenosine triphosphatase, a process for determining biological cells contained in foods and beverages in convenient and precise manner in combination with bioluminescence method, and a reagent for the determination thereof.
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8.发明公开Bioluminescence reagent and method for quantitative determination of adenosine phosphate ester using the reagent 失效Bioluminiszenzreagens和方法，用于使用该试剂Andenosinphosphatestern的定量测定
公开(公告)号：EP0794260A1
公开(公告)日：1997-09-10
申请号：EP97103430.1
申请日：1997-03-03
申请人： KIKKOMAN CORPORATION
摘要： There is provided a bioluminescence reagent comprising at least pyruvate orthophosphate dikinase, phosphoenolpyruvic acid, pyrophosphoric acid, magnesium ion or another metallic ions, luciferin and luciferase, which reagent is such that the amount of luminescence is maintained in a high level and moreover stably without decaying for a long time in a bioluminescence reaction, and there is provided a method for quantitatively determining an adenosine phosphate ester or a substance taking part in the ATP conversion reaction in high sensitivity and high accuracy using an inexpensive and simple measuring apparatus.
摘要翻译： 提供了一种生物发光试剂，其包含至少丙酮酸正磷酸二激酶，磷酸烯醇式丙酮酸，焦磷酸，镁离子或其它金属离子，萤光素和萤光素酶，其中试剂被检查做发光量保持在较高的水平，更超过平稳，无衰减 用于在生物发光反应时间长，且有对腺苷磷酸酯或使用在便宜和简单的测量装置，在高灵敏度和高准确度的ATP转化反应的物质参与的定量确定性采矿的方法。
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