公开(公告)号：EP3379248A1

公开(公告)日：2018-09-26

申请号：EP16866421.7

申请日：2016-11-18

申请人： Tokyo Ohka Kogyo Co., Ltd. ,  Tabata, Yasuhiko

发明人： MAENO, Emi

IPC分类号： G01N33/50 ,  C12Q1/34

CPC分类号： C12Q1/34 ,  G01N2333/978 ,  G01N2500/20

摘要： The present invention provides an arginase activity measurement method that easily and conveniently measures arginase activity with high sensitivity. The arginase activity measurement method includes a step of adding a sample including arginase and a solution including a divalent cation to a reaction vessel, and thereby activating arginase; a step of adding an arginine solution to the sample including the activated arginase, and performing an enzyme-substrate reaction; and a step of adding an acidic solution including a urea detection reagent and having a pH of from 1.0 to 4.0 to the sample subjected to the enzyme-substrate reaction, and simultaneously performing deactivation of enzyme and a urea detection reaction.

公开(公告)号：EP2689024A2

公开(公告)日：2014-01-29

申请号：EP12710995.7

申请日：2012-03-26

申请人： Almac Sciences (Scotland) Limited

发明人： COTTON, Graham ,  DUNSMORE, Colin

IPC分类号： C12Q1/00

CPC分类号： C12Q1/37 ,  C12Q1/26 ,  C12Q1/34 ,  C12Q1/48 ,  G01N2333/90245 ,  G01N2333/91011 ,  G01N2333/91057 ,  G01N2333/978

摘要： The invention is concerned with methods of assaying the activity of enzymes based on measurement of fluorescence lifetime (FLT). In particular, the invention relates to assays for enzymes which are capable of modifying the structure of peptide substrates, including for example enzymes catalysing methylation, demethylation, acetylation, deacetylation or deimination of peptide substrates.

公开(公告)号：EP2292275A2

公开(公告)日：2011-03-09

申请号：EP10184001.5

申请日：2001-10-11

申请人： UNIVERSITY OF SOUTH CAROLINA

发明人： Roberts, Joseph ,  Sethuraman, Natarajan

IPC分类号： A61K49/00 ,  A61K47/48 ,  G01N33/68

CPC分类号： G01N33/582 ,  A61K38/50 ,  C12N9/96 ,  C12Y305/01038 ,  G01N33/5047 ,  G01N33/5088 ,  G01N33/573 ,  G01N2333/21 ,  G01N2333/978 ,  G01N2469/10

摘要： The present invention relates to a method for determining the modification conditions of a therapeutic agent comprising (1) assaying the biological activity of a first modified therapeutic agent after the first modified therapeutic agent has been administered to a subject; (2) assaying the biological activity of the first modified therapeutic agent after at least one booster dose of the first modified therapeutic agent has been administered to said subject (3) carrying out (1) and (2) with an additional modified therapeutic agent that has been modified differently than the first modified therapeutic agent, and (4) comparing the biological activity of the first modified therapeutic agent with the biological activity of the additional modified therapeutic agent. The present invention also relates to modified therapeutic agents.

摘要翻译： 本发明涉及一种用于测定治疗剂的修饰条件的方法，包括（1）在将第一改性治疗剂施用于受试者之后测定第一修饰治疗剂的生物活性; （2）在向所述受试者（3）施用至少一种加强剂量的第一修饰治疗剂后，测定第一修饰治疗剂的生物活性，（3）进行（1）和（2）的另外的改良治疗剂， 已经与第一修饰治疗剂不同地进行了修饰，和（4）比较第一修饰治疗剂的生物活性与其它改性治疗剂的生物活性。 本发明还涉及改良的治疗剂。

公开(公告)号：EP1895012A1

公开(公告)日：2008-03-05

申请号：EP06018120.3

申请日：2006-08-30

申请人： Universitätsklinikum Freiburg

发明人： Bauer, Georg

IPC分类号： C12Q1/00 ,  A61K31/496 ,  A61K31/4196 ,  A61K31/415 ,  A61K31/4174 ,  A61P35/00

CPC分类号： G01N33/5011 ,  A61K31/19 ,  A61K31/195 ,  A61K31/415 ,  A61K31/4174 ,  A61K31/4196 ,  A61K31/496 ,  C12Q1/30 ,  G01N2333/908 ,  G01N2333/978 ,  G01N2510/00 ,  A61K2300/00

摘要： The present invention refers to a method for inducing tumor apoptosis by influencing the ROS (reactive oxygen species) signaling pathway in tumor cells. Increasing the level of ROS leads to the selective inactivation of a tumor cell catalase and subsequently to an apoptosis of these cells. The level of ROS can be increased by increasing the level of nitric oxide through inhibition of the enzymes nitric oxide dioxygenase or arginase.
According to the present invention inhibitors of the nitric oxide dioxygenase or arginase can be used for the manufacture of a medicament for the treatment of cancer. The present invention further provides a method for identifying compounds which can be used for the treatment of cancer, wherein the method allows to specifically identify compounds which induce apoptosis through the ROS signaling pathway. The present invention also provides a kit for identifying compounds which induce tumor apoptosis by inactivating a catalase on the tumor cell surface.

摘要翻译： 本发明涉及通过影响肿瘤细胞中的ROS（活性氧）信号通路诱导肿瘤细胞凋亡的方法。 增加ROS的水平导致肿瘤细胞过氧化氢酶的选择性失活，然后导致这些细胞的凋亡。 通过抑制一氧化氮双加氧酶或精氨酸酶来增加一氧化氮的水平可以提高ROS的水平。 根据本发明，一氧化氮双加氧酶或精氨酸酶的抑制剂可用于制备用于治疗癌症的药物。 本发明还提供了鉴定可用于治疗癌症的化合物的方法，其中所述方法允许特异性鉴定通过ROS信号通路诱导细胞凋亡的化合物。 本发明还提供了一种用于鉴定通过灭活肿瘤细胞表面上的过氧化氢酶诱导肿瘤细胞凋亡的化合物的试剂盒。

公开(公告)号：EP1851328A1

公开(公告)日：2007-11-07

申请号：EP06710346.5

申请日：2006-02-01

申请人： Warner-Lambert Company LLC

发明人： WISNER, Kyunghye, Ahn Pfizer Global Research and

IPC分类号： C12Q1/34

CPC分类号： C12Q1/32 ,  C12Q1/34 ,  G01N2333/978 ,  G01N2500/00

摘要： The invention provides methods for determining the activity of an ammonia­generating enzyme and methods for identifying a compound capable of modulating the activity of such an enzyme.

公开(公告)号：EP1765316A2

公开(公告)日：2007-03-28

申请号：EP05788968.5

申请日：2005-06-16

申请人： The President and Fellows of Harvard College

发明人： SINCLAIR, David, A. ,  COHEN, Haim, Y.

IPC分类号： A61K31/05 ,  C12Q1/68 ,  G01N33/574 ,  C07H21/04 ,  C12P21/06 ,  C12N9/80 ,  C12N9/99

CPC分类号： C12Q1/25 ,  C12Q1/34 ,  G01N33/5011 ,  G01N33/57484 ,  G01N33/6872 ,  G01N2333/978 ,  G01N2500/02 ,  G01N2510/00

摘要： Provided herein are methods and compositions for modulating apoptosis of cells and the lifespan of cells. These may be used for treating or preventing aging-related disorders and cancer.

公开(公告)号：EP1527196A4

公开(公告)日：2007-03-14

申请号：EP03711317

申请日：2003-02-28

申请人： CHILDRENS HOSP MEDICAL CENTER

发明人： ROTHENBERG MARC ,  ZIMMERMANN NIVES

IPC分类号： C12N15/09 ,  A61K31/00 ,  A61K31/198 ,  A61K45/00 ,  A61P11/06 ,  A61P37/08 ,  A61P43/00 ,  C12Q1/34 ,  C12Q1/68 ,  G01N33/68

CPC分类号： C12Q1/6883 ,  A61K31/00 ,  A61K31/198 ,  C12Q1/34 ,  C12Q2600/158 ,  G01N33/6893 ,  G01N2333/978 ,  G01N2500/04 ,  G01N2800/122 ,  G01N2800/24

公开(公告)号：EP1132467A2

公开(公告)日：2001-09-12

申请号：EP01113052.3

申请日：1997-02-13

申请人： Toyo Boseki Kabushiki Kaisha

发明人： Sogabe, Atsushi, c/oToyo Boseki Kabushiki Kaisha ,  Hattori, Takashi, c/oToyo Boseki Kabushiki Kaisha ,  Nishiya, Yoshiaki, c/oToyo Boseki Kabushiki Kaisha ,  Kawamura, Yoshihisa, c/oToyo Boseki Kabushiki K.

IPC分类号： C12N9/78 ,  C12N9/80 ,  C12Q1/34

CPC分类号： G01N33/52 ,  C12Q1/34 ,  G01N2333/978 ,  Y10S435/829

摘要： A creatine amidinohydrolase having the following physicochemical properties:

Action: catalyzing the following reaction; creatine + H 2 O → sarcosine + urea
Optimum temperature: about 40 - 50°C
Optimum pH: pH about 8.0 - 9.0
Heat stability: not more than about 50°C (pH 7.5, 30 min)
Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5 - 10.0 mM
Molecular weight: about 43,000 (SDS-PAGE)
Isoelectric point: about 3.5,
a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor. According to the present invention, a creatine amidinohydrolase having a smaller Km value than that of the conventionally known creatine amidinohydrolase can be produced in an industrially large amount, and can be used as a routine reagent for clinical tests for determining creatine and creatinine in biological samples.

摘要翻译： 具有以下物理化学性质的肌酸脒基水解酶：作用：催化下列反应; 肌酸+ H 2 O→肌氨酸+尿素最适温度：约40-50℃最适pH：pH约8.0-9.0热稳定性：不超过约50℃（pH7.5,30分钟）偶联测定中使用的Km值 肌氨酸氧化酶和过氧化物酶：约3.5-10.0mM分子量：约43,000（SDS-PAGE）等电点：约3.5，生产所述酶的方法，包括培养产生所述酶的微生物，测定肌酸 或使用所述酶的样品中的肌酸酐，及其试剂。 根据本发明，可以以工业上大量生产具有比常规已知的肌酸脒基水解酶更小的Km值的肌酸脒基水解酶，并且可以用作用于确定生物样品中的肌酸和肌酸酐的临床测试的常规试剂 。

公开(公告)号：EP0437254A3

公开(公告)日：1993-04-07

申请号：EP91100241.8

申请日：1991-01-09

申请人： BOEHRINGER MANNHEIM GMBH

发明人： Siedel, Joachim, Dr. ,  Vogt, Bernd, Dr.

IPC分类号： C12Q1/34 ,  C12Q1/50

CPC分类号： C12Q1/34 ,  C12Q1/50 ,  G01N2333/978 ,  G01N2333/98

摘要： Zur enzymatischen Bestimmung von Creatinin in wäßrigen Lösungen setzt man das in der Probe enthaltene Creatinin mittels der Creatinin-Iminohydrolase zu 1-Methylhydantoin und einem ersten Molekül NH₃ um, überführt das gebildete 1-Methylhydantoin mittels der ATP-abhängigen 1-Methylhydantoinase und der N-Carbamoylsarcosin-Amidohydrolase in Sarcosin, CO₂ und ein zweites Molekül NH₃ und bestimmt beide gebildeten Moleküle NH₃ gemeinsam.

公开(公告)号：EP0437254A2

公开(公告)日：1991-07-17

申请号：EP91100241.8

申请日：1991-01-09

申请人： BOEHRINGER MANNHEIM GMBH

发明人： Siedel, Joachim, Dr. ,  Vogt, Bernd, Dr.

IPC分类号： C12Q1/34 ,  C12Q1/50

CPC分类号： C12Q1/34 ,  C12Q1/50 ,  G01N2333/978 ,  G01N2333/98

摘要： Zur enzymatischen Bestimmung von Creatinin in wäßrigen Lösungen setzt man das in der Probe enthaltene Creatinin mittels der Creatinin-Iminohydrolase zu 1-Methylhydantoin und einem ersten Molekül NH₃ um, überführt das gebildete 1-Methylhydantoin mittels der ATP-abhängigen 1-Methylhydantoinase und der N-Carbamoylsarcosin-Amidohydrolase in Sarcosin, CO₂ und ein zweites Molekül NH₃ und bestimmt beide gebildeten Moleküle NH₃ gemeinsam.

摘要翻译： 为了在水溶液中酶测定肌酐，样品中含有的肌酸酐通过肌酐亚氨基水解酶转化为1-甲基乙内酰脲和第一分子NH 3，形成的1-甲基乙内酰脲通过ATP依赖性1-甲基乙内酰脲酶 和N-氨基甲酰基肌氨酰氨基水解酶分解成肌氨酸，二氧化碳和第二分子的NH 3，形成的两个分子的NH 3一起测定。