Improved method for the expression of biotin in bacillus sphaericus
    4.
    发明公开
    Improved method for the expression of biotin in bacillus sphaericus 失效
    Verfahren zur表达von生物素在球形芽孢杆菌。

    公开(公告)号:EP0379428A1

    公开(公告)日:1990-07-25

    申请号:EP90400129.4

    申请日:1990-01-17

    IPC分类号: C12N15/01 C12N15/52 C12P17/18

    摘要: The present invention relates to a method for the selection of organisms derepressed in the expression of primary or secondary metabolites, starting with a parent strain of this normally repressed organism, wherein:
    - said parent strain is transformed with a plasmid carrying a marker gene under the control of the DNA sequence involved in the regulation of the expression of said metabolite,
    - the strains thus transformed are subjected to a mutagenic treatment, and
    - the positive strains expressing said marker in the presence of said metabolite at a concentration totally repressing the expression of said marker in the transformed parent strain are selected,
    - from among these positive strains, the strains hyperproductive of metabolite are selected, option­ally after they have been relieved of their plas­mids.

    摘要翻译: 本发明涉及一种选择在这种通常被抑制的生物体的亲本菌株开始的初级或次级代谢物的表达中去除阻滞的生物体的方法,其中: - 所述亲本菌株用携带标记基因的质粒转化 参与调节所述代谢物表达的DNA序列的控制, - 如此转化的菌株进行诱变处理, - 在所述代谢物存在下表达所述标记的阳性菌株,其浓度完全抑制 选择转化的亲本菌株中的所述标记物, - 从这些阳性菌株中选择代谢物的高产菌株,任选地在它们已经释放其质粒之后。