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    NANOPORE DETECTION OF SMALL MOLECULES THROUGH COMPETITION ASSAYS
    2.
    发明公开
    NANOPORE DETECTION OF SMALL MOLECULES THROUGH COMPETITION ASSAYS 审中-公开

    公开(公告)号：EP3268732A4

    公开(公告)日：2018-07-25

    申请号：EP16762680

    申请日：2016-03-11

    申请人： TWO PORE GUYS INC

    发明人： MORIN TREVOR J

    IPC分类号： G01N33/00 ,  C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53

    CPC分类号： G01N33/54366 ,  C12Q1/6816 ,  G01N33/48721 ,  G01N33/54306 ,  C12Q2563/119 ,  C12Q2563/155 ,  C12Q2563/167 ,  C12Q2565/631

    摘要： Disclosed herein are methods and compositions for detection of target small molecules in a mixed sample by performing a competition assay between the target and a surrogate and subsequently detecting the complex types in a nanopore device. Disclosed herein are competition assays for detection of small molecules using a nanopore. Target molecules of a sufficient size (> 20 kDa) when passed through a solid-state nanopore cause a change in the current impedance, translocation time, or other measurable parameter. In the event the target molecule is not sufficiently big, and thus does not cause a noticeable change, an additional molecule/reagent can be used to aid in detection. This detection reagent would bind to the small molecule or to the "capture ligand-molecule complex" (e.g. peptide detection is aided by a monoclonal antibody (mAb) that recognizes a peptide/aptamer complex).