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1.发明公开
公开(公告)号：EP3934765A1
公开(公告)日：2022-01-12
申请号：EP20770558.3
申请日：2020-03-06
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公开(公告)号：EP3325670A2
公开(公告)日：2018-05-30
申请号：EP16837439.5
申请日：2016-06-28
IPC分类号： C12Q1/68 , C12N15/11 , C12N15/113 , C12N15/115
CPC分类号： C12N15/1048 , C12N15/1096 , C12N15/113 , C12N15/115 , C12N2310/12 , C12N2310/16 , C12N2330/31
摘要： Provided herein, among other things, is an automatable procedure that employs in vitro directed evolution to create DNA sequences that encode a ligand-responsive ribozyme and which, when transcribed, can control expression of genes they are coupled to. The method also allows creation of functional RNA sequences that bind target molecules, without requiring any modification or immobilization of the target.
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公开(公告)号：EP4314301A1
公开(公告)日：2024-02-07
申请号：EP22776568.2
申请日：2022-03-23
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4.发明公开
公开(公告)号：EP3770267A1
公开(公告)日：2021-01-27
申请号：EP20194808.0
申请日：2014-11-03
摘要： Host cells that are engineered to produce benzylisoquinoline alkaloid (BIAs) precursors, such as norcoclaurine (NC) and norlaudanosoline (NL), are provided. The host cells may have one or more engineered modifications selected from: a feedback inhibition alleviating mutation in a enzyme gene; a transcriptional modulation modification of a biosynthetic enzyme gene; an inactivating mutation in an enzyme; and a heterologous coding sequence. Also provided are methods of producing a BIA of interest or a precursor thereof using the host cells and compositions, e.g., kits, systems etc., that find use in methods of the invention.
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5.发明公开BENZYLISOQUINOLINE ALKALOID (BIA) PRECURSOR PRODUCING MICROBES, AND METHODS OF MAKING AND USING THE SAME 审中-公开生产微生物的苯甲酰喹啉生物碱（BIA）前体，以及制备和使用该生物微生物的方法
公开(公告)号：EP3292203A1
公开(公告)日：2018-03-14
申请号：EP16790024.0
申请日：2016-05-04
CPC分类号： C12N15/52 , C07D217/18 , C07D217/22 , C12N15/79 , C12N15/81 , C12P13/22 , C12P17/12 , C12Y114/16002 , C12Y401/01028
摘要： Methods and engineered yeast cells for generating a benzylisoquinoline alkaloid product are provided herein. A method comprises providing engineered yeast cells and a feedstock to a reactor. In the reactor, the engineered yeast cells are subjected to fermentation by incubating the engineered yeast cells for a time period to produce a solution comprising the BIA product and cellular material. The solution comprises not more than one class of molecule selected from the group of protoberberine, morphinan, isopavine, aporphine, and benzylisoquinoline. Additionally, at least one separation unit is used to separate the BIA product from the cellular material to provide the product stream comprising the BIA product.
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6.发明公开
公开(公告)号：EP3221461A1
公开(公告)日：2017-09-27
申请号：EP15861129.3
申请日：2015-11-16
发明人： SMOLKE, Christina D. , LI, Yanran
IPC分类号： C12P13/16
CPC分类号： C12P17/188 , C07D273/01 , C07D515/02 , C07D515/14 , C07D515/22 , C12N1/02 , C12N1/14 , C12N9/1003 , C12N2330/50 , C12P13/16
摘要： Engineered non-plant cells that produce a benzylisoquinoline alkaloid product that is a derivative of canadine along a metabolic pathway that converts canadine, or an analog of canadine, to a noscapinoid product are provided. Methods of culturing engineered non-plant cells that produce a noscapinoid product and pharmaceutical compositions are also provided.
摘要翻译： 提供了产生苄基异喹啉生物碱产物的经改造的非植物细胞，所述苄基异喹啉生物碱产物是沿着代替途径将canadine或canadine类似物转化为非类甾体产物的代谢途径的衍生物。 还提供了培养产生非索皮生类产品的工程化非植物细胞和药物组合物的方法。
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