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    LIGAND/BINDING PARTNER BIO-LABELING SYSTEMS
    3.
    发明公开
    LIGAND/BINDING PARTNER BIO-LABELING SYSTEMS 审中-公开
    配体/结合伙伴生物标记系统

    公开(公告)号：EP1601576A2

    公开(公告)日：2005-12-07

    申请号：EP04716415.7

    申请日：2004-03-02

    申请人： The Trustees of Columbia University in the City of New York

    发明人： CORNISH, Virginia ,  SHEETZ, Michael ,  MILLER, Larry

    IPC分类号： B65C1/00

    CPC分类号： G01N33/582 ,  C12Q1/025 ,  C12Q1/32 ,  G01N33/5005 ,  G01N33/573 ,  G01N2333/906 ,  G01N2500/10

    摘要： The present invention relates to methods and compositions which utilize binding relationships between detector molecules and their ligands for labeling molecules of interest in vivo. A 'target' molecule is linked to a detector molecule (such as a protein or a nucleic acid) and the function and/or location of the target molecule is monitored by binding the detector molecule its ligand, which carries a detectable label. In particular embodiments of the invention, an intracellular fusion protein comprising a target protein and a detector protein is bound to a membrane-permeable, fluorescently-labeled ligand of the detector protein, thereby providing an adjunct or alternative to Green Fluorescent Protein.

    摘要翻译： 本发明涉及利用检测器分子和它们的配体之间的结合关系在体内标记感兴趣的分子的方法和组合物。 “目标”分子与检测分子（例如蛋白质或核酸）相连接，并且通过将检测分子与其配体（其携带可检测标记）结合来监测靶分子的功能和/或位置。 在本发明的具体实施方案中，包含靶蛋白和检测蛋白的细胞内融合蛋白与检测器蛋白的膜渗透性荧光标记的配体结合，由此提供绿色荧光蛋白的添加剂或替代物。