利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

13 条记录 (耗时 0.017 秒)

    Improvements in the expression of newly introduced genes in yeast cells
    3.
    发明公开
    Improvements in the expression of newly introduced genes in yeast cells 失效
    在YEAST细胞中新引入基因表达的改进

    公开(公告)号：EP0129268A3

    公开(公告)日：1985-06-19

    申请号：EP84200735

    申请日：1984-05-21

    申请人： UNILEVER NV ,  UNILEVER PLC

    发明人： Edens, Luppo ,  Russell, Stephen William ,  Visser, Christiaan ,  Verrips, Cornelis Theodorus

    IPC分类号： C12N15/00 ,  C12P21/02 ,  C07H21/04 ,  C12N01/16 ,  C12R01/645

    CPC分类号： C12N9/6481 ,  C07K14/43 ,  C07K2319/00 ,  C07K2319/02 ,  C12N15/625 ,  C12N15/81 ,  C12N15/815

    摘要： The invention relates to an improved expression system for newly introduced genes in yeast and comprises a yeast regulon, and preferably a transcription terminator, derived from one of the GAPDH genes of S. cerevisiae. The about 850 nucleotides long GAPDH regulon described proved to be almost ten times as effective as smaller regulons described previously. Said regulon and/orterminator can be introduced into yeasts either as part of plasmid molecules or by incorporating into the yeast genome. Vectors containing the expression system preferably comprise an autonomously replicating sequence derived from K. lactis (KARS) or an origin of replication originating from the S. cerevisiae 2 micron yeast plasmid. After transformation of yeasts, in particular of the genera Kluyveromyces and Saccharomyces, with said vectors, the yeasts transformed can produce foreign proteins more effectively. In the case ofthaumatin the presence of signal polypeptide (the pre-part) appears to be essential for expression in yeast. DNA sequence encoding other signal polypeptides are also described. The use of codons preferred by yeast in both structural genes, preferably those encoding thaumatin- like proteins and chymosin-like proteins, and DNA sequences encoding signal polypeptides is also described.

    Improvements in the expression of newly introduced genes in yeast cells
    8.
    发明公开
    Improvements in the expression of newly introduced genes in yeast cells 失效
    Hefezellen的“堕落者”（Genesis）中的“Verbesserungen in der表达”von neueingeführtenGenen。

    公开(公告)号：EP0129268A2

    公开(公告)日：1984-12-27

    申请号：EP84200735.3

    申请日：1984-05-21

    申请人： UNILEVER N.V. ,  UNILEVER PLC

    发明人： Edens, Luppo ,  Russell, Stephen William ,  Visser, Christiaan ,  Verrips, Cornelis Theodorus

    IPC分类号： C12N15/00 ,  C12P21/02 ,  C07H21/04 ,  C12N1/16

    CPC分类号： C12N9/6481 ,  C07K14/43 ,  C07K2319/00 ,  C07K2319/02 ,  C12N15/625 ,  C12N15/81 ,  C12N15/815

    摘要： The invention relates to an improved expression system for newly introduced genes in yeast and comprises a yeast regulon, and preferably a transcription terminator, derived from one of the GAPDH genes of S. cerevisiae. The about 850 nucleotides long GAPDH regulon described proved to be almost ten times as effective as smaller regulons described previously. Said regulon and/orterminator can be introduced into yeasts either as part of plasmid molecules or by incorporating into the yeast genome.
    Vectors containing the expression system preferably comprise an autonomously replicating sequence derived from K. lactis (KARS) or an origin of replication originating from the S. cerevisiae 2 micron yeast plasmid.
    After transformation of yeasts, in particular of the genera Kluyveromyces and Saccharomyces, with said vectors, the yeasts transformed can produce foreign proteins more effectively.
    In the case ofthaumatin the presence of signal polypeptide (the pre-part) appears to be essential for expression in yeast. DNA sequence encoding other signal polypeptides are also described. The use of codons preferred by yeast in both structural genes, preferably those encoding thaumatin- like proteins and chymosin-like proteins, and DNA sequences encoding signal polypeptides is also described.

    摘要翻译： 本发明涉及一种用于酵母中新引入的基因的改进的表达系统，其包含衍生自酿酒酵母的GAPDH基因之一的酵母调节子，优选转录终止子。 所描述的大约850个核苷酸长的GAPDH调节子证明是前面描述的较小的调节子的几十倍。 所述调节子和/或终止子可以作为质粒分子的一部分引入酵母中，或通过掺入酵母基因组中。 含有表达系统的载体优选包含衍生自乳酸克鲁维酵母（KARS）的自主复制序列或源自酿酒酵母2微米酵母质粒的复制起点。 在酵母转化后，尤其是克鲁维酵母属和酵母属，用所述载体转化后，转化的酵母可以更有效地产生外源蛋白质。 在奇异果甜蛋白的情况下，信号多肽（前体部分）的存在似乎对于在酵母中表达是必需的。 还描述了编码其他信号多肽的DNA序列。 还描述了在两种结构基因中优选使用密码子，优选编码类似花露蛋白的蛋白质和凝乳细胞样蛋白的密码子，以及编码信号多肽的DNA序列。